Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity

Bacillus thuringiensis subsp. israelensis (Bti) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a Bti strain isolated from Qatar QBT220 genes encoding the δ-endotoxins responsible of the ins...

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Autores principales:	Kavita Nair, Roda Al-Thani, Samir Jaoua
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Bacillus thuringiensis PBtoxis plasmid instability Bioinsecticide production Environmental pollution TD172-193.5 Environmental sciences GE1-350
Acceso en línea:	https://doaj.org/article/edd89bffcfbd430b96e90e66136bc51a
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spelling	oai:doaj.org-article:edd89bffcfbd430b96e90e66136bc51a2021-11-12T04:24:17ZBacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity0147-651310.1016/j.ecoenv.2021.112975https://doaj.org/article/edd89bffcfbd430b96e90e66136bc51a2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S0147651321010873https://doaj.org/toc/0147-6513Bacillus thuringiensis subsp. israelensis (Bti) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a Bti strain isolated from Qatar QBT220 genes encoding the δ-endotoxins responsible of the insecticidal activities revealed the alteration of a 14-kb DNA region including the δ-endotoxins cry10A and cyt1C genes of pBtoxis plasmid. The presence of all the insecticidal genes except cry10A and cyt1C was explained by a structural instability of the plasmid pBtoxis. However, when compared with the Bti reference strains H14 and QBT217 that carry all δ-endotoxins coding genes, it was found that QBT220, has a significantly higher insecticidal activity against the dipteran insect Aedes aegypti larvae despite of the plasmid pBtoxis structural instability due to the alteration of cry10A and cyt1C genes. In addition, QBT220 showed the highest δ-endotoxin synthesis per spore, compared with that of the wildtype strains. These findings confirm that the altered genes cry10A and cyt1C are not mandatory for Bti insecticidal activities and on the other hand show a possible inhibitory effect played by the 2 proteins Cry10A and Cyt1C on the insecticidal activities of the other insecticidal proteins. In addition, the QBT220 increased δ-endotoxins synthesis per cell, makes this strain a good candidate for possible applications in the industrial production of bioinsecticides.Kavita NairRoda Al-ThaniSamir JaouaElsevierarticleBacillus thuringiensisPBtoxis plasmid instabilityBioinsecticide productionEnvironmental pollutionTD172-193.5Environmental sciencesGE1-350ENEcotoxicology and Environmental Safety, Vol 228, Iss , Pp 112975- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Bacillus thuringiensis PBtoxis plasmid instability Bioinsecticide production Environmental pollution TD172-193.5 Environmental sciences GE1-350
spellingShingle	Bacillus thuringiensis PBtoxis plasmid instability Bioinsecticide production Environmental pollution TD172-193.5 Environmental sciences GE1-350 Kavita Nair Roda Al-Thani Samir Jaoua Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
description	Bacillus thuringiensis subsp. israelensis (Bti) spherical parasporal crystal contains several insecticidal proteins used as environmentally safe alternative to toxic chemical pesticides. The exploration of a Bti strain isolated from Qatar QBT220 genes encoding the δ-endotoxins responsible of the insecticidal activities revealed the alteration of a 14-kb DNA region including the δ-endotoxins cry10A and cyt1C genes of pBtoxis plasmid. The presence of all the insecticidal genes except cry10A and cyt1C was explained by a structural instability of the plasmid pBtoxis. However, when compared with the Bti reference strains H14 and QBT217 that carry all δ-endotoxins coding genes, it was found that QBT220, has a significantly higher insecticidal activity against the dipteran insect Aedes aegypti larvae despite of the plasmid pBtoxis structural instability due to the alteration of cry10A and cyt1C genes. In addition, QBT220 showed the highest δ-endotoxin synthesis per spore, compared with that of the wildtype strains. These findings confirm that the altered genes cry10A and cyt1C are not mandatory for Bti insecticidal activities and on the other hand show a possible inhibitory effect played by the 2 proteins Cry10A and Cyt1C on the insecticidal activities of the other insecticidal proteins. In addition, the QBT220 increased δ-endotoxins synthesis per cell, makes this strain a good candidate for possible applications in the industrial production of bioinsecticides.
format	article
author	Kavita Nair Roda Al-Thani Samir Jaoua
author_facet	Kavita Nair Roda Al-Thani Samir Jaoua
author_sort	Kavita Nair
title	Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
title_short	Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
title_full	Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
title_fullStr	Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
title_full_unstemmed	Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
title_sort	bacillus thuringiensis strain qbt220 pbtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/edd89bffcfbd430b96e90e66136bc51a
work_keys_str_mv	AT kavitanair bacillusthuringiensisstrainqbt220pbtoxisplasmidstructuralinstabilityenhancesdendotoxinssynthesisandbioinsecticidalactivity AT rodaalthani bacillusthuringiensisstrainqbt220pbtoxisplasmidstructuralinstabilityenhancesdendotoxinssynthesisandbioinsecticidalactivity AT samirjaoua bacillusthuringiensisstrainqbt220pbtoxisplasmidstructuralinstabilityenhancesdendotoxinssynthesisandbioinsecticidalactivity
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Bacillus thuringiensis strain QBT220 pBtoxis plasmid structural instability enhances δ-endotoxins synthesis and bioinsecticidal activity

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