Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro

Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro

Jayakumar Jerobin, Pooja Makwana, RS Suresh Kumar, Rajiv Sundaramoorthy, Amitava Mukherjee, Natarajan Chandrasekaran Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India Abstract: Neem (Azadirachta indica) is recognized as a medicinal plant well known for its antibacterial, ant...

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Autores principales: Jerobin J, Makwana P, Suresh Kumar RS, Sundaramoorthy R, Mukherjee A, Chandrasekaran N
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2015
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/edde1c782c98428db9dff191bbdec999
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spelling oai:doaj.org-article:edde1c782c98428db9dff191bbdec9992021-12-02T08:11:16ZAntibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro1178-2013https://doaj.org/article/edde1c782c98428db9dff191bbdec9992015-10-01T00:00:00Zhttps://www.dovepress.com/antibacterial-activity-of-neem-nanoemulsion-and-its-toxicity-assessmen-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Jayakumar Jerobin, Pooja Makwana, RS Suresh Kumar, Rajiv Sundaramoorthy, Amitava Mukherjee, Natarajan Chandrasekaran Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India Abstract: Neem (Azadirachta indica) is recognized as a medicinal plant well known for its antibacterial, antimalarial, antiviral, and antifungal properties. Neem nanoemulsion (NE) (O/W) is formulated using neem oil, Tween 20, and water by high-energy ultrasonication. The formulated neem NE showed antibacterial activity against the bacterial pathogen Vibrio vulnificus by disrupting the integrity of the bacterial cell membrane. Despite the use of neem NE in various biomedical applications, the toxicity studies on human cells are still lacking. The neem NE showed a decrease in cellular viability in human lymphocytes after 24 hours of exposure. The neem NE at lower concentration (0.7–1 mg/mL) is found to be nontoxic while it is toxic at higher concentrations (1.2–2 mg/mL). The oxidative stress induced by the neem NE is evidenced by the depletion of catalase, SOD, and GSH levels in human lymphocytes. Neem NE showed a significant increase in DNA damage when compared to control in human lymphocytes (P<0.05). The NE is an effective antibacterial agent against the bacterial pathogen V. vulnificus, and it was found to be nontoxic at lower concentrations to human lymphocytes. Keywords: neem, nanoemulsion, antibacterial, lymphocytes, cytotoxicity, genotoxicityJerobin JMakwana PSuresh Kumar RSSundaramoorthy RMukherjee AChandrasekaran NDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss Supplement 1 Challenges in biomaterials research, Pp 77-86 (2015)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Jerobin J
Makwana P
Suresh Kumar RS
Sundaramoorthy R
Mukherjee A
Chandrasekaran N
Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
description Jayakumar Jerobin, Pooja Makwana, RS Suresh Kumar, Rajiv Sundaramoorthy, Amitava Mukherjee, Natarajan Chandrasekaran Centre for Nanobiotechnology, VIT University, Vellore, Tamil Nadu, India Abstract: Neem (Azadirachta indica) is recognized as a medicinal plant well known for its antibacterial, antimalarial, antiviral, and antifungal properties. Neem nanoemulsion (NE) (O/W) is formulated using neem oil, Tween 20, and water by high-energy ultrasonication. The formulated neem NE showed antibacterial activity against the bacterial pathogen Vibrio vulnificus by disrupting the integrity of the bacterial cell membrane. Despite the use of neem NE in various biomedical applications, the toxicity studies on human cells are still lacking. The neem NE showed a decrease in cellular viability in human lymphocytes after 24 hours of exposure. The neem NE at lower concentration (0.7–1 mg/mL) is found to be nontoxic while it is toxic at higher concentrations (1.2–2 mg/mL). The oxidative stress induced by the neem NE is evidenced by the depletion of catalase, SOD, and GSH levels in human lymphocytes. Neem NE showed a significant increase in DNA damage when compared to control in human lymphocytes (P<0.05). The NE is an effective antibacterial agent against the bacterial pathogen V. vulnificus, and it was found to be nontoxic at lower concentrations to human lymphocytes. Keywords: neem, nanoemulsion, antibacterial, lymphocytes, cytotoxicity, genotoxicity
format article
author Jerobin J
Makwana P
Suresh Kumar RS
Sundaramoorthy R
Mukherjee A
Chandrasekaran N
author_facet Jerobin J
Makwana P
Suresh Kumar RS
Sundaramoorthy R
Mukherjee A
Chandrasekaran N
author_sort Jerobin J
title Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
title_short Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
title_full Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
title_fullStr Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
title_full_unstemmed Antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
title_sort antibacterial activity of neem nanoemulsion and its toxicity assessment on human lymphocytes in vitro
publisher Dove Medical Press
publishDate 2015
url https://doaj.org/article/edde1c782c98428db9dff191bbdec999
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AT sundaramoorthyr antibacterialactivityofneemnanoemulsionanditstoxicityassessmentonhumanlymphocytesinnbspvitro
AT mukherjeea antibacterialactivityofneemnanoemulsionanditstoxicityassessmentonhumanlymphocytesinnbspvitro
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