Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy

Fengqiu Liu,1,2 Yuli Chen,1,2 Yizhen Li,1,2 Yuan Guo,1,2 Yang Cao,1,2 Pan Li,1,2 Zhigang Wang,1,2 Yuping Gong,1,2,* Haitao Ran1,2,* 1Ultrasound Department, Second Affiliated Hospital, Chongqing Medical University, 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing, China *These a...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Liu F, Chen Y, Li Y, Guo Y, Cao Y, Li P, Wang Z, Gong Y, Ran H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2018
Materias:	nanomedicine folate receptor-targeted nanoparticle theranostics targeted drug delivery system combined anti-cancer therapy photoacoustic imaging ultrasound imaging Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ede5e21d56894186bac29c3d20d4fd73
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ede5e21d56894186bac29c3d20d4fd73
record_format	dspace
spelling	oai:doaj.org-article:ede5e21d56894186bac29c3d20d4fd732021-12-02T02:55:17ZFolate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy1178-2013https://doaj.org/article/ede5e21d56894186bac29c3d20d4fd732018-09-01T00:00:00Zhttps://www.dovepress.com/folate-receptor-targeted-laser-activable-polylactide-co-glycolic-acid--peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Fengqiu Liu,1,2 Yuli Chen,1,2 Yizhen Li,1,2 Yuan Guo,1,2 Yang Cao,1,2 Pan Li,1,2 Zhigang Wang,1,2 Yuping Gong,1,2,* Haitao Ran1,2,* 1Ultrasound Department, Second Affiliated Hospital, Chongqing Medical University, 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing, China *These authors contributed equally to this work Background: Cancer is one of the most serious threats to human health. Precision medicine is an innovative approach to treatment, as part of which theranostic nanomedicine has been studied extensively. However, the required biocompatibility and substantial cost for the approval of nanomedicines hinder their clinical translation. Purpose: We designed a novel type of theranostic nanoparticle (NP) folate-receptor-targeted laser-activatable poly(lactide-co-glycolic acid) (PLGA) NPs loaded with paclitaxel (Ptx)/indocyanine green (ICG)-folic acid-polyethylene glycol (PEG)-PLGA-Ptx@ICG-perfluorohexane (Pfh)- using safe and approved materials and drugs, which would facilitate clinical translation. With laser irradiation, highly efficient photothermal therapy can be achieved. Additionally, targeted NPs can be activated by near-infrared laser irradiation at a specific region, which leads to the sharp release of Ptx at areas of high folate-receptor expression and ensures a higher Ptx concentration within the tumor region, thereby leading to chemo/photothermal synergistic antitumor efficacy. Meanwhile, the NPs can be used as a dual-modality contrast agent for photoacoustic and ultrasound imaging. Materials and methods: FA-PEG-PLGA-Ptx@ICG-Pfh NPs were prepared by sonification method and characterized for physicochemical properties. Cytotoxicity and in vivo biocompatibility were evaluated respectively by CCK8 assay and blood analysis. NPs as dual-modality contrast agents were evaluated by photoacoustic/ultrasound imaging system in vitro and in vivo. In vitro anticancer effect and in vivo anticancer therapy was evaluated by CCK8 assay and MDA-MB231 tumor-bearing mice model. Results: FA-PEG-PLGA-Ptx@ICG-Pfh NPs were in the size of 308±5.82 nm with negative zeta potential and showed excellent photothermal effect. The NPs could be triggered sharp release of Ptx by laser irradiation, and showed the good biocompatibility in vitro and in vivo. Through photoacoustic/ultrasound imaging, the NPs showed an excellent ability as dual-modality contrast agents in vitro and in vivo. FA-PEG-PLGA-Ptx@ICG-Pfh NPs with laser irradiation showed the best anticancer efficacy in vitro and in vivo. Conclusion: Such a biocompatible and novel theranostic NP is expected to integrate dual-modality imaging with improved therapeutic efficacy and provide a promising paradigm for cancer therapy. Keywords: nanomedicine, folate-receptor-targeted nanoparticle, theranostics, targeted drug-delivery system, combined anticancer therapy, photoacoustic imaging, ultrasound imagingLiu FChen YLi YGuo YCao YLi PWang ZGong YRan HDove Medical Pressarticlenanomedicinefolate receptor-targeted nanoparticletheranosticstargeted drug delivery systemcombined anti-cancer therapyphotoacoustic imagingultrasound imagingMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 13, Pp 5139-5158 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	nanomedicine folate receptor-targeted nanoparticle theranostics targeted drug delivery system combined anti-cancer therapy photoacoustic imaging ultrasound imaging Medicine (General) R5-920
spellingShingle	nanomedicine folate receptor-targeted nanoparticle theranostics targeted drug delivery system combined anti-cancer therapy photoacoustic imaging ultrasound imaging Medicine (General) R5-920 Liu F Chen Y Li Y Guo Y Cao Y Li P Wang Z Gong Y Ran H Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
description	Fengqiu Liu,1,2 Yuli Chen,1,2 Yizhen Li,1,2 Yuan Guo,1,2 Yang Cao,1,2 Pan Li,1,2 Zhigang Wang,1,2 Yuping Gong,1,2,* Haitao Ran1,2,* 1Ultrasound Department, Second Affiliated Hospital, Chongqing Medical University, 2Chongqing Key Laboratory of Ultrasound Molecular Imaging, Chongqing, China *These authors contributed equally to this work Background: Cancer is one of the most serious threats to human health. Precision medicine is an innovative approach to treatment, as part of which theranostic nanomedicine has been studied extensively. However, the required biocompatibility and substantial cost for the approval of nanomedicines hinder their clinical translation. Purpose: We designed a novel type of theranostic nanoparticle (NP) folate-receptor-targeted laser-activatable poly(lactide-co-glycolic acid) (PLGA) NPs loaded with paclitaxel (Ptx)/indocyanine green (ICG)-folic acid-polyethylene glycol (PEG)-PLGA-Ptx@ICG-perfluorohexane (Pfh)- using safe and approved materials and drugs, which would facilitate clinical translation. With laser irradiation, highly efficient photothermal therapy can be achieved. Additionally, targeted NPs can be activated by near-infrared laser irradiation at a specific region, which leads to the sharp release of Ptx at areas of high folate-receptor expression and ensures a higher Ptx concentration within the tumor region, thereby leading to chemo/photothermal synergistic antitumor efficacy. Meanwhile, the NPs can be used as a dual-modality contrast agent for photoacoustic and ultrasound imaging. Materials and methods: FA-PEG-PLGA-Ptx@ICG-Pfh NPs were prepared by sonification method and characterized for physicochemical properties. Cytotoxicity and in vivo biocompatibility were evaluated respectively by CCK8 assay and blood analysis. NPs as dual-modality contrast agents were evaluated by photoacoustic/ultrasound imaging system in vitro and in vivo. In vitro anticancer effect and in vivo anticancer therapy was evaluated by CCK8 assay and MDA-MB231 tumor-bearing mice model. Results: FA-PEG-PLGA-Ptx@ICG-Pfh NPs were in the size of 308±5.82 nm with negative zeta potential and showed excellent photothermal effect. The NPs could be triggered sharp release of Ptx by laser irradiation, and showed the good biocompatibility in vitro and in vivo. Through photoacoustic/ultrasound imaging, the NPs showed an excellent ability as dual-modality contrast agents in vitro and in vivo. FA-PEG-PLGA-Ptx@ICG-Pfh NPs with laser irradiation showed the best anticancer efficacy in vitro and in vivo. Conclusion: Such a biocompatible and novel theranostic NP is expected to integrate dual-modality imaging with improved therapeutic efficacy and provide a promising paradigm for cancer therapy. Keywords: nanomedicine, folate-receptor-targeted nanoparticle, theranostics, targeted drug-delivery system, combined anticancer therapy, photoacoustic imaging, ultrasound imaging
format	article
author	Liu F Chen Y Li Y Guo Y Cao Y Li P Wang Z Gong Y Ran H
author_facet	Liu F Chen Y Li Y Guo Y Cao Y Li P Wang Z Gong Y Ran H
author_sort	Liu F
title	Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
title_short	Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
title_full	Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
title_fullStr	Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
title_full_unstemmed	Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
title_sort	folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy
publisher	Dove Medical Press
publishDate	2018
url	https://doaj.org/article/ede5e21d56894186bac29c3d20d4fd73
work_keys_str_mv	AT liuf folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT cheny folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT liy folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT guoy folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT caoy folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT lip folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT wangz folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT gongy folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy AT ranh folatereceptortargetedlaseractivablepolylactidecoglycolicacidnanoparticlesloadedwithpaclitaxelindocyaninegreenforphotoacousticultrasoundimagingandchemophotothermaltherapy
_version_	1718402077284106240

Folate-receptor-targeted laser-activable poly(lactide-co-glycolic acid) nanoparticles loaded with paclitaxel/indocyanine green for photoacoustic/ultrasound imaging and chemo/photothermal therapy

Ejemplares similares