Human age-declined saliva metabolic markers determined by LC–MS

Abstract Metabolites in human biofluids reflect individual physiological states influenced by various factors. Using liquid chromatography-mass spectrometry (LC–MS), we conducted non-targeted, non-invasive metabolomics using saliva of 27 healthy volunteers in Okinawa, comprising 13 young (30 ± 3 yea...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Takayuki Teruya, Haruhisa Goga, Mitsuhiro Yanagida
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edea6e5599774a9a9122104cd53835b8
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:edea6e5599774a9a9122104cd53835b8
record_format	dspace
spelling	oai:doaj.org-article:edea6e5599774a9a9122104cd53835b82021-12-02T18:34:02ZHuman age-declined saliva metabolic markers determined by LC–MS10.1038/s41598-021-97623-72045-2322https://doaj.org/article/edea6e5599774a9a9122104cd53835b82021-09-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-97623-7https://doaj.org/toc/2045-2322Abstract Metabolites in human biofluids reflect individual physiological states influenced by various factors. Using liquid chromatography-mass spectrometry (LC–MS), we conducted non-targeted, non-invasive metabolomics using saliva of 27 healthy volunteers in Okinawa, comprising 13 young (30 ± 3 year) and 14 elderly (76 ± 4 year) subjects. Few studies have comprehensively identified age-dependent changes in salivary metabolites. Among 99 salivary metabolites, 21 were statistically age-related. All of the latter decline in abundance with advancing age, except ATP, which increased 1.96-fold in the elderly, possibly due to reduced ATP consumption. Fourteen age-linked and highly correlated compounds function in a metabolic network involving the pentose-phosphate pathway, glycolysis/gluconeogenesis, amino acids, and purines/pyrimidines nucleobases. The remaining seven less strongly correlated metabolites, include ATP, anti-oxidation-related glutathione disulfide, muscle-related acetyl-carnosine, N-methyl-histidine, creatinine, RNA-related dimethyl-xanthine and N-methyl-adenosine. In addition, glutamate and N-methyl-histidine are related to taste, so their decline suggests that the elderly lose some ability to taste. Reduced redox metabolism and muscle activity are suggested by changes in glutathione and acetyl-carnosine. These age-linked salivary metabolites together illuminate a metabolic network that reflects a decline of oral functions during human aging.Takayuki TeruyaHaruhisa GogaMitsuhiro YanagidaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-11 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Takayuki Teruya Haruhisa Goga Mitsuhiro Yanagida Human age-declined saliva metabolic markers determined by LC–MS
description	Abstract Metabolites in human biofluids reflect individual physiological states influenced by various factors. Using liquid chromatography-mass spectrometry (LC–MS), we conducted non-targeted, non-invasive metabolomics using saliva of 27 healthy volunteers in Okinawa, comprising 13 young (30 ± 3 year) and 14 elderly (76 ± 4 year) subjects. Few studies have comprehensively identified age-dependent changes in salivary metabolites. Among 99 salivary metabolites, 21 were statistically age-related. All of the latter decline in abundance with advancing age, except ATP, which increased 1.96-fold in the elderly, possibly due to reduced ATP consumption. Fourteen age-linked and highly correlated compounds function in a metabolic network involving the pentose-phosphate pathway, glycolysis/gluconeogenesis, amino acids, and purines/pyrimidines nucleobases. The remaining seven less strongly correlated metabolites, include ATP, anti-oxidation-related glutathione disulfide, muscle-related acetyl-carnosine, N-methyl-histidine, creatinine, RNA-related dimethyl-xanthine and N-methyl-adenosine. In addition, glutamate and N-methyl-histidine are related to taste, so their decline suggests that the elderly lose some ability to taste. Reduced redox metabolism and muscle activity are suggested by changes in glutathione and acetyl-carnosine. These age-linked salivary metabolites together illuminate a metabolic network that reflects a decline of oral functions during human aging.
format	article
author	Takayuki Teruya Haruhisa Goga Mitsuhiro Yanagida
author_facet	Takayuki Teruya Haruhisa Goga Mitsuhiro Yanagida
author_sort	Takayuki Teruya
title	Human age-declined saliva metabolic markers determined by LC–MS
title_short	Human age-declined saliva metabolic markers determined by LC–MS
title_full	Human age-declined saliva metabolic markers determined by LC–MS
title_fullStr	Human age-declined saliva metabolic markers determined by LC–MS
title_full_unstemmed	Human age-declined saliva metabolic markers determined by LC–MS
title_sort	human age-declined saliva metabolic markers determined by lc–ms
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/edea6e5599774a9a9122104cd53835b8
work_keys_str_mv	AT takayukiteruya humanagedeclinedsalivametabolicmarkersdeterminedbylcms AT haruhisagoga humanagedeclinedsalivametabolicmarkersdeterminedbylcms AT mitsuhiroyanagida humanagedeclinedsalivametabolicmarkersdeterminedbylcms
_version_	1718377953376600064

Human age-declined saliva metabolic markers determined by LC–MS

Ejemplares similares