Electrochemical studies of lateral flow assay test results for procalcitonin detection

In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conj...

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Autores principales:	Yachana Gupta Gupta, Kalpana, Aditya Sharma Ghrera
Formato:	article
Lenguaje:	EN
Publicado:	International Association of Physical Chemists (IAPC) 2021
Materias:	Cyclic voltammetry lateral flow assay gold nanoparticles polyclonal antibody monoclonal antibody biomarker Chemistry QD1-999
Acceso en línea:	https://doaj.org/article/edf22c3f4faa43838ce86c8f29eaeaa2
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spelling	oai:doaj.org-article:edf22c3f4faa43838ce86c8f29eaeaa22021-11-29T10:18:47ZElectrochemical studies of lateral flow assay test results for procalcitonin detection10.5599/jese.11271847-9286https://doaj.org/article/edf22c3f4faa43838ce86c8f29eaeaa22021-11-01T00:00:00Zhttps://pub.iapchem.org/ojs/index.php/JESE/article/view/1127https://doaj.org/toc/1847-9286 In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conjugates with antibodies specific to the model antigen PCT are assessed. Polyclonal antibody (pAb) labelled with gold nanoparticles (AuNP) to obtain the AuNP-pAb complex and the specific monoclonal antibody (mAb) have been dropped at the test zone. This complex is placed over the conjugate line of the LFA strip. In the absence of PCT or the presence of other biomarkers, the test line remained colourless, which revealed the specificity of assay towards PCT among a pool of various analytes. Herein, observations have been made through two different platforms for quantitative and qualitative analysis for the detection of PCT biomarker. The qualitative analysis has been performed on the basis of appearance red color in the test band, while for quantitative analysis, a novel approach has been adopted. Herein, the nitrocellulose membrane (paper strip) is cut out from the LFA strip and used for electrochemical studies under similar solution conditions. Different paper strips presented different cyclic voltammograms (CV) that could be correlated to varying PCT concentrations captured at the test line of the paper strip. The qualitative detection limit for PCT using this LFA was determined to be 2 ng/ml and the quantitative detection limit was 1 ng/ml. The electrochemical response studies of the paper strip by CV technique revealed the sensitivity value of 0.695 mA ng/ml. Yachana Gupta GuptaKalpanaAditya Sharma GhreraInternational Association of Physical Chemists (IAPC)articleCyclic voltammetrylateral flow assaygold nanoparticlespolyclonal antibodymonoclonal antibodybiomarkerChemistryQD1-999ENJournal of Electrochemical Science and Engineering, Vol 11, Iss 3 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cyclic voltammetry lateral flow assay gold nanoparticles polyclonal antibody monoclonal antibody biomarker Chemistry QD1-999
spellingShingle	Cyclic voltammetry lateral flow assay gold nanoparticles polyclonal antibody monoclonal antibody biomarker Chemistry QD1-999 Yachana Gupta Gupta Kalpana Aditya Sharma Ghrera Electrochemical studies of lateral flow assay test results for procalcitonin detection
description	In this study, the lateral flow assay (LFA) has been developed for the detection of bacterial infection (BI) by specific biomarker procalcitonin (PCT), without a need for complicated instrumentations and technical expertise. For the development of the assay, gold nanoparticles (AuNP) and their conjugates with antibodies specific to the model antigen PCT are assessed. Polyclonal antibody (pAb) labelled with gold nanoparticles (AuNP) to obtain the AuNP-pAb complex and the specific monoclonal antibody (mAb) have been dropped at the test zone. This complex is placed over the conjugate line of the LFA strip. In the absence of PCT or the presence of other biomarkers, the test line remained colourless, which revealed the specificity of assay towards PCT among a pool of various analytes. Herein, observations have been made through two different platforms for quantitative and qualitative analysis for the detection of PCT biomarker. The qualitative analysis has been performed on the basis of appearance red color in the test band, while for quantitative analysis, a novel approach has been adopted. Herein, the nitrocellulose membrane (paper strip) is cut out from the LFA strip and used for electrochemical studies under similar solution conditions. Different paper strips presented different cyclic voltammograms (CV) that could be correlated to varying PCT concentrations captured at the test line of the paper strip. The qualitative detection limit for PCT using this LFA was determined to be 2 ng/ml and the quantitative detection limit was 1 ng/ml. The electrochemical response studies of the paper strip by CV technique revealed the sensitivity value of 0.695 mA ng/ml.
format	article
author	Yachana Gupta Gupta Kalpana Aditya Sharma Ghrera
author_facet	Yachana Gupta Gupta Kalpana Aditya Sharma Ghrera
author_sort	Yachana Gupta Gupta
title	Electrochemical studies of lateral flow assay test results for procalcitonin detection
title_short	Electrochemical studies of lateral flow assay test results for procalcitonin detection
title_full	Electrochemical studies of lateral flow assay test results for procalcitonin detection
title_fullStr	Electrochemical studies of lateral flow assay test results for procalcitonin detection
title_full_unstemmed	Electrochemical studies of lateral flow assay test results for procalcitonin detection
title_sort	electrochemical studies of lateral flow assay test results for procalcitonin detection
publisher	International Association of Physical Chemists (IAPC)
publishDate	2021
url	https://doaj.org/article/edf22c3f4faa43838ce86c8f29eaeaa2
work_keys_str_mv	AT yachanaguptagupta electrochemicalstudiesoflateralflowassaytestresultsforprocalcitonindetection AT kalpana electrochemicalstudiesoflateralflowassaytestresultsforprocalcitonindetection AT adityasharmaghrera electrochemicalstudiesoflateralflowassaytestresultsforprocalcitonindetection
_version_	1718407438595522560

Electrochemical studies of lateral flow assay test results for procalcitonin detection

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