An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.

Hepatitis C virus (HCV) is a global problem. To better understand HCV infection researchers employ in vitro HCV cell-culture (HCVcc) systems that use Huh-7 derived hepatoma cells that are particularly permissive to HCV infection. A variety of hyper-permissive cells have been subcloned for this purpo...

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Autores principales:	Jamie I MacPherson, Ben Sidders, Stefan Wieland, Jin Zhong, Paul Targett-Adams, Volker Lohmann, Perdita Backes, Oona Delpuech-Adams, Francis Chisari, Marilyn Lewis, Tanya Parkinson, David L Robertson
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2011
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edf278f29c554c29a9a20b69865ee1a6
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spelling	oai:doaj.org-article:edf278f29c554c29a9a20b69865ee1a62021-11-18T07:35:49ZAn integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.1932-620310.1371/journal.pone.0025584https://doaj.org/article/edf278f29c554c29a9a20b69865ee1a62011-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/22046242/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Hepatitis C virus (HCV) is a global problem. To better understand HCV infection researchers employ in vitro HCV cell-culture (HCVcc) systems that use Huh-7 derived hepatoma cells that are particularly permissive to HCV infection. A variety of hyper-permissive cells have been subcloned for this purpose. In addition, subclones of Huh-7 which have evolved resistance to HCV are available. However, the mechanisms of susceptibility or resistance to infection among these cells have not been fully determined. In order to elucidate mechanisms by which hepatoma cells are susceptible or resistant to HCV infection we performed genome-wide expression analyses of six Huh-7 derived cell cultures that have different levels of permissiveness to infection. A great number of genes, representing a wide spectrum of functions are differentially expressed between cells. To focus our investigation, we identify host proteins from HCV replicase complexes, perform gene expression analysis of three HCV infected cells and conduct a detailed analysis of differentially expressed host factors by integrating a variety of data sources. Our results demonstrate that changes relating to susceptibility to HCV infection in hepatoma cells are linked to the innate immune response, secreted signal peptides and host factors that have a role in virus entry and replication. This work identifies both known and novel host factors that may influence HCV infection. Our findings build upon current knowledge of the complex interplay between HCV and the host cell, which could aid development of new antiviral strategies.Jamie I MacPhersonBen SiddersStefan WielandJin ZhongPaul Targett-AdamsVolker LohmannPerdita BackesOona Delpuech-AdamsFrancis ChisariMarilyn LewisTanya ParkinsonDavid L RobertsonPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 6, Iss 10, p e25584 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Jamie I MacPherson Ben Sidders Stefan Wieland Jin Zhong Paul Targett-Adams Volker Lohmann Perdita Backes Oona Delpuech-Adams Francis Chisari Marilyn Lewis Tanya Parkinson David L Robertson An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.
description	Hepatitis C virus (HCV) is a global problem. To better understand HCV infection researchers employ in vitro HCV cell-culture (HCVcc) systems that use Huh-7 derived hepatoma cells that are particularly permissive to HCV infection. A variety of hyper-permissive cells have been subcloned for this purpose. In addition, subclones of Huh-7 which have evolved resistance to HCV are available. However, the mechanisms of susceptibility or resistance to infection among these cells have not been fully determined. In order to elucidate mechanisms by which hepatoma cells are susceptible or resistant to HCV infection we performed genome-wide expression analyses of six Huh-7 derived cell cultures that have different levels of permissiveness to infection. A great number of genes, representing a wide spectrum of functions are differentially expressed between cells. To focus our investigation, we identify host proteins from HCV replicase complexes, perform gene expression analysis of three HCV infected cells and conduct a detailed analysis of differentially expressed host factors by integrating a variety of data sources. Our results demonstrate that changes relating to susceptibility to HCV infection in hepatoma cells are linked to the innate immune response, secreted signal peptides and host factors that have a role in virus entry and replication. This work identifies both known and novel host factors that may influence HCV infection. Our findings build upon current knowledge of the complex interplay between HCV and the host cell, which could aid development of new antiviral strategies.
format	article
author	Jamie I MacPherson Ben Sidders Stefan Wieland Jin Zhong Paul Targett-Adams Volker Lohmann Perdita Backes Oona Delpuech-Adams Francis Chisari Marilyn Lewis Tanya Parkinson David L Robertson
author_facet	Jamie I MacPherson Ben Sidders Stefan Wieland Jin Zhong Paul Targett-Adams Volker Lohmann Perdita Backes Oona Delpuech-Adams Francis Chisari Marilyn Lewis Tanya Parkinson David L Robertson
author_sort	Jamie I MacPherson
title	An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.
title_short	An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.
title_full	An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.
title_fullStr	An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.
title_full_unstemmed	An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.
title_sort	integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to hcv infection.
publisher	Public Library of Science (PLoS)
publishDate	2011
url	https://doaj.org/article/edf278f29c554c29a9a20b69865ee1a6
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An integrated transcriptomic and meta-analysis of hepatoma cells reveals factors that influence susceptibility to HCV infection.

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