The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria

Abstract Lactic acid bacteria (LAB) are used as a probiotic alternative to antibiotics in livestock production. Microencapsulation technology is widely used for probiotic preservation. A variety of microencapsulation protocols have been proposed and compared based on chemicals and mechanical procedu...

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Autores principales:	Pawiya Pupa, Prasert Apiwatsiri, Wandee Sirichokchatchawan, Nopadon Pirarat, Nongnuj Muangsin, Asad Ali Shah, Nuvee Prapasarakul
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edf28a4c595541b6a2ff769d46408ed6
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spelling	oai:doaj.org-article:edf28a4c595541b6a2ff769d46408ed62021-12-02T18:18:32ZThe efficacy of three double-microencapsulation methods for preservation of probiotic bacteria10.1038/s41598-021-93263-z2045-2322https://doaj.org/article/edf28a4c595541b6a2ff769d46408ed62021-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-93263-zhttps://doaj.org/toc/2045-2322Abstract Lactic acid bacteria (LAB) are used as a probiotic alternative to antibiotics in livestock production. Microencapsulation technology is widely used for probiotic preservation. A variety of microencapsulation protocols have been proposed and compared based on chemicals and mechanical procedures. This study aimed to develop a double-encapsulated coating from alginate (1.5%) and chitosan (0.5%) by extrusion, emulsion, and spray drying methods using the LAB strains Lactobacillus plantarum strains 31F, 25F, 22F, Pediococcus pentosaceus 77F, and P. acidilactici 72N, and to monitor the basic probiotic properties of the encapsulated prototypes. The final products from each microencapsulation protocol were analysed for their appearance, probiotic properties and viable cell count. Using the spray drying method, particles smaller than 15 μm in diameter with a regular spherical shape were obtained, whereas the other methods produced larger (1.4–52 mm) and irregularly shaped microcapsules. After storage for 6 months at room temperature, the LAB viability of the spray-dried particles was the highest among the three methods. In all the LAB strains examined, the encapsulated LAB retained their probiotic properties in relation to acid-bile tolerance and antibacterial activity. This study highlights the efficacy of double-coating microencapsulation for preserving LAB properties and survival rate, and demonstrates its potential for probiotic application in livestock farms.Pawiya PupaPrasert ApiwatsiriWandee SirichokchatchawanNopadon PiraratNongnuj MuangsinAsad Ali ShahNuvee PrapasarakulNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Pawiya Pupa Prasert Apiwatsiri Wandee Sirichokchatchawan Nopadon Pirarat Nongnuj Muangsin Asad Ali Shah Nuvee Prapasarakul The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
description	Abstract Lactic acid bacteria (LAB) are used as a probiotic alternative to antibiotics in livestock production. Microencapsulation technology is widely used for probiotic preservation. A variety of microencapsulation protocols have been proposed and compared based on chemicals and mechanical procedures. This study aimed to develop a double-encapsulated coating from alginate (1.5%) and chitosan (0.5%) by extrusion, emulsion, and spray drying methods using the LAB strains Lactobacillus plantarum strains 31F, 25F, 22F, Pediococcus pentosaceus 77F, and P. acidilactici 72N, and to monitor the basic probiotic properties of the encapsulated prototypes. The final products from each microencapsulation protocol were analysed for their appearance, probiotic properties and viable cell count. Using the spray drying method, particles smaller than 15 μm in diameter with a regular spherical shape were obtained, whereas the other methods produced larger (1.4–52 mm) and irregularly shaped microcapsules. After storage for 6 months at room temperature, the LAB viability of the spray-dried particles was the highest among the three methods. In all the LAB strains examined, the encapsulated LAB retained their probiotic properties in relation to acid-bile tolerance and antibacterial activity. This study highlights the efficacy of double-coating microencapsulation for preserving LAB properties and survival rate, and demonstrates its potential for probiotic application in livestock farms.
format	article
author	Pawiya Pupa Prasert Apiwatsiri Wandee Sirichokchatchawan Nopadon Pirarat Nongnuj Muangsin Asad Ali Shah Nuvee Prapasarakul
author_facet	Pawiya Pupa Prasert Apiwatsiri Wandee Sirichokchatchawan Nopadon Pirarat Nongnuj Muangsin Asad Ali Shah Nuvee Prapasarakul
author_sort	Pawiya Pupa
title	The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
title_short	The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
title_full	The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
title_fullStr	The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
title_full_unstemmed	The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
title_sort	efficacy of three double-microencapsulation methods for preservation of probiotic bacteria
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/edf28a4c595541b6a2ff769d46408ed6
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The efficacy of three double-microencapsulation methods for preservation of probiotic bacteria

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