Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever

Abstract Dengue virus causes dengue hemorrhagic fever (DHF) and has been associated to fatal cases worldwide. The liver is one of the most important target tissues in severe cases, due to its intense viral replication and metabolic role. microRNAs role during infection is crucial to understand the r...

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Autores principales:	Layanna Freitas de Oliveira, Amanda Araújo Serrão de Andrade, Carla Pagliari, Leda Viegas de Carvalho, Taiana S. Silveira, Jedson Ferreira Cardoso, André Luiz Teles e Silva, Janaina Mota de Vasconcelos, Caroline Aquino Moreira-Nunes, Rommel Mario Rodríguez Burbano, Márcio Roberto Teixeira Nunes, Eduardo José Melo dos Santos, João Lídio da Silva Gonçalves Vianez Júnior
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/edf566d1f7724ed99f9710ec7b9758a1
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spelling	oai:doaj.org-article:edf566d1f7724ed99f9710ec7b9758a12021-12-02T13:20:04ZDifferential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever10.1038/s41598-020-72892-w2045-2322https://doaj.org/article/edf566d1f7724ed99f9710ec7b9758a12021-03-01T00:00:00Zhttps://doi.org/10.1038/s41598-020-72892-whttps://doaj.org/toc/2045-2322Abstract Dengue virus causes dengue hemorrhagic fever (DHF) and has been associated to fatal cases worldwide. The liver is one of the most important target tissues in severe cases, due to its intense viral replication and metabolic role. microRNAs role during infection is crucial to understand the regulatory mechanisms of DENV infection and can help in diagnostic and anti-viral therapies development. We sequenced the miRNome of six fatal cases and compared to five controls, to characterize the human microRNAs expression profile in the liver tissue during DHF. Eight microRNAs were differentially expressed, including miR-126-5p, a regulatory molecule of endothelial cells, miR-122-5p, a liver specific homeostasis regulator, and miR-146a-5p, an interferon-regulator. Enrichment analysis with predicted target genes of microRNAs revealed regulatory pathways of apoptosis, involving MAPK, RAS, CDK and FAS. Immune response pathways were related to NF- kB, CC and CX families, IL and TLR. This is the first description of the human microRNA and isomicroRNA profile in liver tissues from DHF cases. The results demonstrated the association of miR-126-5p, miR-122-5p and miR-146a-5p with DHF liver pathogenesis, involving endothelial repair and vascular permeability regulation, control of homeostasis and expression of inflammatory cytokines.Layanna Freitas de OliveiraAmanda Araújo Serrão de AndradeCarla PagliariLeda Viegas de CarvalhoTaiana S. SilveiraJedson Ferreira CardosoAndré Luiz Teles e SilvaJanaina Mota de VasconcelosCaroline Aquino Moreira-NunesRommel Mario Rodríguez BurbanoMárcio Roberto Teixeira NunesEduardo José Melo dos SantosJoão Lídio da Silva Gonçalves Vianez JúniorNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-9 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Layanna Freitas de Oliveira Amanda Araújo Serrão de Andrade Carla Pagliari Leda Viegas de Carvalho Taiana S. Silveira Jedson Ferreira Cardoso André Luiz Teles e Silva Janaina Mota de Vasconcelos Caroline Aquino Moreira-Nunes Rommel Mario Rodríguez Burbano Márcio Roberto Teixeira Nunes Eduardo José Melo dos Santos João Lídio da Silva Gonçalves Vianez Júnior Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever
description	Abstract Dengue virus causes dengue hemorrhagic fever (DHF) and has been associated to fatal cases worldwide. The liver is one of the most important target tissues in severe cases, due to its intense viral replication and metabolic role. microRNAs role during infection is crucial to understand the regulatory mechanisms of DENV infection and can help in diagnostic and anti-viral therapies development. We sequenced the miRNome of six fatal cases and compared to five controls, to characterize the human microRNAs expression profile in the liver tissue during DHF. Eight microRNAs were differentially expressed, including miR-126-5p, a regulatory molecule of endothelial cells, miR-122-5p, a liver specific homeostasis regulator, and miR-146a-5p, an interferon-regulator. Enrichment analysis with predicted target genes of microRNAs revealed regulatory pathways of apoptosis, involving MAPK, RAS, CDK and FAS. Immune response pathways were related to NF- kB, CC and CX families, IL and TLR. This is the first description of the human microRNA and isomicroRNA profile in liver tissues from DHF cases. The results demonstrated the association of miR-126-5p, miR-122-5p and miR-146a-5p with DHF liver pathogenesis, involving endothelial repair and vascular permeability regulation, control of homeostasis and expression of inflammatory cytokines.
format	article
author	Layanna Freitas de Oliveira Amanda Araújo Serrão de Andrade Carla Pagliari Leda Viegas de Carvalho Taiana S. Silveira Jedson Ferreira Cardoso André Luiz Teles e Silva Janaina Mota de Vasconcelos Caroline Aquino Moreira-Nunes Rommel Mario Rodríguez Burbano Márcio Roberto Teixeira Nunes Eduardo José Melo dos Santos João Lídio da Silva Gonçalves Vianez Júnior
author_facet	Layanna Freitas de Oliveira Amanda Araújo Serrão de Andrade Carla Pagliari Leda Viegas de Carvalho Taiana S. Silveira Jedson Ferreira Cardoso André Luiz Teles e Silva Janaina Mota de Vasconcelos Caroline Aquino Moreira-Nunes Rommel Mario Rodríguez Burbano Márcio Roberto Teixeira Nunes Eduardo José Melo dos Santos João Lídio da Silva Gonçalves Vianez Júnior
author_sort	Layanna Freitas de Oliveira
title	Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever
title_short	Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever
title_full	Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever
title_fullStr	Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever
title_full_unstemmed	Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever
title_sort	differential expression analysis and profiling of hepatic mirna and isomirna in dengue hemorrhagic fever
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/edf566d1f7724ed99f9710ec7b9758a1
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Differential expression analysis and profiling of hepatic miRNA and isomiRNA in dengue hemorrhagic fever

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