New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2

Soad A Mohamad,1,* Eman Maher Zahran,2,* Maha Raafat Abdel Fadeel,3 Amgad Albohy,4 Mohamed A Safwat5 1Department of Pharmaceutics, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt; 2Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universiti...

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Autores principales:	Mohamad SA, Zahran EM, Abdel Fadeel MR, Albohy A, Safwat MA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2021
Materias:	acaciin molecular docking nanofibers core-shell bcv/sars-cov-2 Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/edf8dea044cc40d099f62171e8aff482
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spelling	oai:doaj.org-article:edf8dea044cc40d099f62171e8aff4822021-12-02T14:40:41ZNew Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-21178-2013https://doaj.org/article/edf8dea044cc40d099f62171e8aff4822021-03-01T00:00:00Zhttps://www.dovepress.com/new-acaciin-loaded-self-assembled-nanofibers-as-mpro-inhibitors-agains-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013Soad A Mohamad,1,* Eman Maher Zahran,2,* Maha Raafat Abdel Fadeel,3 Amgad Albohy,4 Mohamed A Safwat5 1Department of Pharmaceutics, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt; 2Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt; 3Veterinary Serum and Vaccine Research Institute (VSVRI), Cairo, Egypt; 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The British University in Egypt (BUE), El-Sherouk City, 1837, Egypt; 5Department of Pharmaceutics, Faculty of Pharmacy, South Valley University, Qena, 83523, Egypt*These authors contributed equally to this workCorrespondence: Eman Maher ZahranDeraya University, Universities Zone, New Minia City, 61111, EgyptEmail emanzahran84@yahoo.comBackground: SARS-COVID-2 has recently been one of the most life-threatening problems which urgently needs new therapeutic antiviral agents, especially those of herbal origin.Purpose: The study aimed to load acaciin (ACA) into the new self-assembled nanofibers (NFs) followed by investigating their possible antiviral effect against bovine coronavirus (BCV) as a surrogate model for SARS-COV-2.Methods: ACA was identified using 1H-NMR and DEPT-Q 13C-NMR spectroscopy, the molecular docking study was performed using Autodock 4 and a modification of the traditional solvent injection method was applied for the synthesis of the biodegradable NFs. Different characterization techniques were used to inspect the formation of the NFs, which is followed by antiviral investigation against BCV as well as MTT assay using MDBK cells.Results: Core/shell NFs, ranging between 80– 330 nm with tiny thorn-like branches, were formed which attained an enhanced encapsulation efficiency (97.5 ± 0.53%, P< 0.05) and a dual controlled release (a burst release of 65% at 1 h and a sustained release up to > 24 h). The antiviral investigation of the formed NFs revealed a significant inhibition of 98.88 ± 0.16% (P< 0.05) with IC50 of 12.6 μM against BCV cells.Conclusion: The results introduced a new, time/cost-saving strategy for the synthesis of biodegradable NFs without the need for electric current or hazardous cross-linking agents. Moreover, it provided an innovative avenue for the discovery of drugs of herbal origin for the fight against SARS-CoV-2 infection.Keywords: acaciin, molecular docking, nanofibers, core-shell, BCV/SARS-COV-2Mohamad SAZahran EMAbdel Fadeel MRAlbohy ASafwat MADove Medical Pressarticleacaciinmolecular dockingnanofiberscore-shellbcv/sars-cov-2Medicine (General)R5-920ENInternational Journal of Nanomedicine, Vol Volume 16, Pp 1789-1804 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	acaciin molecular docking nanofibers core-shell bcv/sars-cov-2 Medicine (General) R5-920
spellingShingle	acaciin molecular docking nanofibers core-shell bcv/sars-cov-2 Medicine (General) R5-920 Mohamad SA Zahran EM Abdel Fadeel MR Albohy A Safwat MA New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2
description	Soad A Mohamad,1,* Eman Maher Zahran,2,* Maha Raafat Abdel Fadeel,3 Amgad Albohy,4 Mohamed A Safwat5 1Department of Pharmaceutics, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt; 2Department of Pharmacognosy, Faculty of Pharmacy, Deraya University, Universities Zone, New Minia City, 61111, Egypt; 3Veterinary Serum and Vaccine Research Institute (VSVRI), Cairo, Egypt; 4Department of Pharmaceutical Chemistry, Faculty of Pharmacy, The British University in Egypt (BUE), El-Sherouk City, 1837, Egypt; 5Department of Pharmaceutics, Faculty of Pharmacy, South Valley University, Qena, 83523, Egypt*These authors contributed equally to this workCorrespondence: Eman Maher ZahranDeraya University, Universities Zone, New Minia City, 61111, EgyptEmail emanzahran84@yahoo.comBackground: SARS-COVID-2 has recently been one of the most life-threatening problems which urgently needs new therapeutic antiviral agents, especially those of herbal origin.Purpose: The study aimed to load acaciin (ACA) into the new self-assembled nanofibers (NFs) followed by investigating their possible antiviral effect against bovine coronavirus (BCV) as a surrogate model for SARS-COV-2.Methods: ACA was identified using 1H-NMR and DEPT-Q 13C-NMR spectroscopy, the molecular docking study was performed using Autodock 4 and a modification of the traditional solvent injection method was applied for the synthesis of the biodegradable NFs. Different characterization techniques were used to inspect the formation of the NFs, which is followed by antiviral investigation against BCV as well as MTT assay using MDBK cells.Results: Core/shell NFs, ranging between 80– 330 nm with tiny thorn-like branches, were formed which attained an enhanced encapsulation efficiency (97.5 ± 0.53%, P< 0.05) and a dual controlled release (a burst release of 65% at 1 h and a sustained release up to > 24 h). The antiviral investigation of the formed NFs revealed a significant inhibition of 98.88 ± 0.16% (P< 0.05) with IC50 of 12.6 μM against BCV cells.Conclusion: The results introduced a new, time/cost-saving strategy for the synthesis of biodegradable NFs without the need for electric current or hazardous cross-linking agents. Moreover, it provided an innovative avenue for the discovery of drugs of herbal origin for the fight against SARS-CoV-2 infection.Keywords: acaciin, molecular docking, nanofibers, core-shell, BCV/SARS-COV-2
format	article
author	Mohamad SA Zahran EM Abdel Fadeel MR Albohy A Safwat MA
author_facet	Mohamad SA Zahran EM Abdel Fadeel MR Albohy A Safwat MA
author_sort	Mohamad SA
title	New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2
title_short	New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2
title_full	New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2
title_fullStr	New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2
title_full_unstemmed	New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2
title_sort	new acaciin-loaded self-assembled nanofibers as mpro inhibitors against bcv as a surrogate model for sars-cov-2
publisher	Dove Medical Press
publishDate	2021
url	https://doaj.org/article/edf8dea044cc40d099f62171e8aff482
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New Acaciin-Loaded Self-Assembled Nanofibers as MPro Inhibitors Against BCV as a Surrogate Model for SARS-CoV-2

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