Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons

Two large families of olfactory receptors, the Odorant Receptors (ORs) and Ionotropic Receptors (IRs), mediate responses to most odors in the insect olfactory system. Individual odorant binding “tuning” OrX receptors are expressed by olfactory neurons in basiconic and trichoid sensilla and require t...

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Autores principales:	Alina Vulpe, Karen Menuz
Formato:	article
Lenguaje:	EN
Publicado:	Frontiers Media S.A. 2021
Materias:	olfactory receptor Drosophila neuron sensory electrophysiology Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
Acceso en línea:	https://doaj.org/article/edf906ce3e5c4b999dec1390c619610d
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spelling	oai:doaj.org-article:edf906ce3e5c4b999dec1390c619610d2021-11-17T07:00:42ZIr76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons1662-510210.3389/fncel.2021.759238https://doaj.org/article/edf906ce3e5c4b999dec1390c619610d2021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fncel.2021.759238/fullhttps://doaj.org/toc/1662-5102Two large families of olfactory receptors, the Odorant Receptors (ORs) and Ionotropic Receptors (IRs), mediate responses to most odors in the insect olfactory system. Individual odorant binding “tuning” OrX receptors are expressed by olfactory neurons in basiconic and trichoid sensilla and require the co-receptor Orco. The situation for IRs is more complex. Different tuning IrX receptors are expressed by olfactory neurons in coeloconic sensilla and rely on either the Ir25a or Ir8a co-receptors; some evidence suggests that Ir76b may also act as a co-receptor, but its function has not been systematically examined. Surprisingly, recent data indicate that nearly all coeloconic olfactory neurons co-express Ir25a, Ir8a, and Ir76b. Here, we demonstrate that Ir76b and Ir25a function together in all amine-sensing olfactory receptor neurons. In most neurons, loss of either co-receptor abolishes amine responses. In contrast, amine responses persist in the absence of Ir76b or Ir25a in ac1 sensilla but are lost in a double mutant. We show that responses mediated by acid-sensing neurons do not require Ir76b, despite their expression of this co-receptor. Our study also demonstrates that one population of coeloconic olfactory neurons exhibits Ir76b/Ir25a-dependent and Orco-dependent responses to distinct odorants. Together, our data establish the role of Ir76b as a bona fide co-receptor, which acts in partnership with Ir25a. Given that these co-receptors are among the most highly conserved olfactory receptors and are often co-expressed in chemosensory neurons, our data suggest Ir76b and Ir25a also work in tandem in other insects.Alina VulpeKaren MenuzKaren MenuzFrontiers Media S.A.articleolfactoryreceptorDrosophilaneuronsensoryelectrophysiologyNeurosciences. Biological psychiatry. NeuropsychiatryRC321-571ENFrontiers in Cellular Neuroscience, Vol 15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	olfactory receptor Drosophila neuron sensory electrophysiology Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571
spellingShingle	olfactory receptor Drosophila neuron sensory electrophysiology Neurosciences. Biological psychiatry. Neuropsychiatry RC321-571 Alina Vulpe Karen Menuz Karen Menuz Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons
description	Two large families of olfactory receptors, the Odorant Receptors (ORs) and Ionotropic Receptors (IRs), mediate responses to most odors in the insect olfactory system. Individual odorant binding “tuning” OrX receptors are expressed by olfactory neurons in basiconic and trichoid sensilla and require the co-receptor Orco. The situation for IRs is more complex. Different tuning IrX receptors are expressed by olfactory neurons in coeloconic sensilla and rely on either the Ir25a or Ir8a co-receptors; some evidence suggests that Ir76b may also act as a co-receptor, but its function has not been systematically examined. Surprisingly, recent data indicate that nearly all coeloconic olfactory neurons co-express Ir25a, Ir8a, and Ir76b. Here, we demonstrate that Ir76b and Ir25a function together in all amine-sensing olfactory receptor neurons. In most neurons, loss of either co-receptor abolishes amine responses. In contrast, amine responses persist in the absence of Ir76b or Ir25a in ac1 sensilla but are lost in a double mutant. We show that responses mediated by acid-sensing neurons do not require Ir76b, despite their expression of this co-receptor. Our study also demonstrates that one population of coeloconic olfactory neurons exhibits Ir76b/Ir25a-dependent and Orco-dependent responses to distinct odorants. Together, our data establish the role of Ir76b as a bona fide co-receptor, which acts in partnership with Ir25a. Given that these co-receptors are among the most highly conserved olfactory receptors and are often co-expressed in chemosensory neurons, our data suggest Ir76b and Ir25a also work in tandem in other insects.
format	article
author	Alina Vulpe Karen Menuz Karen Menuz
author_facet	Alina Vulpe Karen Menuz Karen Menuz
author_sort	Alina Vulpe
title	Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons
title_short	Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons
title_full	Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons
title_fullStr	Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons
title_full_unstemmed	Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons
title_sort	ir76b is a co-receptor for amine responses in drosophila olfactory neurons
publisher	Frontiers Media S.A.
publishDate	2021
url	https://doaj.org/article/edf906ce3e5c4b999dec1390c619610d
work_keys_str_mv	AT alinavulpe ir76bisacoreceptorforamineresponsesindrosophilaolfactoryneurons AT karenmenuz ir76bisacoreceptorforamineresponsesindrosophilaolfactoryneurons AT karenmenuz ir76bisacoreceptorforamineresponsesindrosophilaolfactoryneurons
_version_	1718425898253811712

Ir76b is a Co-receptor for Amine Responses in Drosophila Olfactory Neurons

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