DNA traces the origin of honey by identifying plants, bacteria and fungi

Abstract The regional origin of a food product commonly affects its value. To this, DNA-based identification of tissue remains could offer fine resolution. For honey, this would allow the usage of not only pollen but all plant tissue, and also that of microbes in the product, for discerning the orig...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Helena Wirta, Nerea Abrego, Kirsten Miller, Tomas Roslin, Eero Vesterinen
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee16763bccb24f0b8850d92d26674a9b
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee16763bccb24f0b8850d92d26674a9b
record_format	dspace
spelling	oai:doaj.org-article:ee16763bccb24f0b8850d92d26674a9b2021-12-02T13:34:57ZDNA traces the origin of honey by identifying plants, bacteria and fungi10.1038/s41598-021-84174-02045-2322https://doaj.org/article/ee16763bccb24f0b8850d92d26674a9b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84174-0https://doaj.org/toc/2045-2322Abstract The regional origin of a food product commonly affects its value. To this, DNA-based identification of tissue remains could offer fine resolution. For honey, this would allow the usage of not only pollen but all plant tissue, and also that of microbes in the product, for discerning the origin. Here we examined how plant, bacterial and fungal taxa identified by DNA metabarcoding and metagenomics differentiate between honey samples from three neighbouring countries. To establish how the taxonomic contents of honey reflect the country of origin, we used joint species distribution modelling. At the lowest taxonomic level by metabarcoding, with operational taxonomic units, the country of origin explained the majority of variation in the data (70–79%), with plant and fungal gene regions providing the clearest distinction between countries. At the taxonomic level of genera, plants provided the most separation between countries with both metabarcoding and metagenomics. The DNA-based methods distinguish the countries more than the morphological pollen identification and the removal of pollen has only a minor effect on taxonomic recovery by DNA. As we find good resolution among honeys from regions with similar biota, DNA-based methods hold great promise for resolving honey origins among more different regions.Helena WirtaNerea AbregoKirsten MillerTomas RoslinEero VesterinenNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Helena Wirta Nerea Abrego Kirsten Miller Tomas Roslin Eero Vesterinen DNA traces the origin of honey by identifying plants, bacteria and fungi
description	Abstract The regional origin of a food product commonly affects its value. To this, DNA-based identification of tissue remains could offer fine resolution. For honey, this would allow the usage of not only pollen but all plant tissue, and also that of microbes in the product, for discerning the origin. Here we examined how plant, bacterial and fungal taxa identified by DNA metabarcoding and metagenomics differentiate between honey samples from three neighbouring countries. To establish how the taxonomic contents of honey reflect the country of origin, we used joint species distribution modelling. At the lowest taxonomic level by metabarcoding, with operational taxonomic units, the country of origin explained the majority of variation in the data (70–79%), with plant and fungal gene regions providing the clearest distinction between countries. At the taxonomic level of genera, plants provided the most separation between countries with both metabarcoding and metagenomics. The DNA-based methods distinguish the countries more than the morphological pollen identification and the removal of pollen has only a minor effect on taxonomic recovery by DNA. As we find good resolution among honeys from regions with similar biota, DNA-based methods hold great promise for resolving honey origins among more different regions.
format	article
author	Helena Wirta Nerea Abrego Kirsten Miller Tomas Roslin Eero Vesterinen
author_facet	Helena Wirta Nerea Abrego Kirsten Miller Tomas Roslin Eero Vesterinen
author_sort	Helena Wirta
title	DNA traces the origin of honey by identifying plants, bacteria and fungi
title_short	DNA traces the origin of honey by identifying plants, bacteria and fungi
title_full	DNA traces the origin of honey by identifying plants, bacteria and fungi
title_fullStr	DNA traces the origin of honey by identifying plants, bacteria and fungi
title_full_unstemmed	DNA traces the origin of honey by identifying plants, bacteria and fungi
title_sort	dna traces the origin of honey by identifying plants, bacteria and fungi
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ee16763bccb24f0b8850d92d26674a9b
work_keys_str_mv	AT helenawirta dnatracestheoriginofhoneybyidentifyingplantsbacteriaandfungi AT nereaabrego dnatracestheoriginofhoneybyidentifyingplantsbacteriaandfungi AT kirstenmiller dnatracestheoriginofhoneybyidentifyingplantsbacteriaandfungi AT tomasroslin dnatracestheoriginofhoneybyidentifyingplantsbacteriaandfungi AT eerovesterinen dnatracestheoriginofhoneybyidentifyingplantsbacteriaandfungi
_version_	1718392769238532096

DNA traces the origin of honey by identifying plants, bacteria and fungi

Ejemplares similares