Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)

Ghrelin can stimulate pituitary growth hormone (GH) release, and has a key role in the regulation of food intake and adiposity in vertebrates. At present, no relevant studies have reported the effects of Ghrelin on intestinal cell proliferation, protein absorption and metabolism. In this paper, firs...

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Autores principales:	Yonghua Zhou, Meixing Guo, Yingjie Li, Yu Zhang, Wenqian Xu, Dafang Zhao, Yuan Chen, Anli Zuo, Fufa Qu, Jianzhou Tang, Shenping Cao, Zhen Liu
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Ctenopharyngodon idella Ghrelin Intestinal cell proliferation Absorption Metabolism Aquaculture. Fisheries. Angling SH1-691
Acceso en línea:	https://doaj.org/article/ee173f2aa1ba4c0a90191f94b748a6a0
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spelling	oai:doaj.org-article:ee173f2aa1ba4c0a90191f94b748a6a02021-11-18T04:50:15ZEffects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)2352-513410.1016/j.aqrep.2021.100928https://doaj.org/article/ee173f2aa1ba4c0a90191f94b748a6a02022-02-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2352513421003446https://doaj.org/toc/2352-5134Ghrelin can stimulate pituitary growth hormone (GH) release, and has a key role in the regulation of food intake and adiposity in vertebrates. At present, no relevant studies have reported the effects of Ghrelin on intestinal cell proliferation, protein absorption and metabolism. In this paper, firstly we established the intestinal epithelial cell lines of juvenile grass carp (Ctenopharyngodon idella) that can be passed stably and identified by the intestinal specific expression proteins E-cadherin and ZO-1. And we used this cell line as a model to investigate the effects of Ghrelin, Ala-Gln, Ala-Gln+Ghrelin on the proliferation of juvenile grass carp intestinal cells. We found that the use of Ghrelin, Ala-Gln and Ala-Gln+Ghrelin could promote the proliferation of intestinal cells of juvenile grass carp, but Ala-Gln+Ghrelin had the strongest promoting effect. Further, we investigated the effects of Ghrelin on the expression of cdx2, PepT1 and mTOR which are related to intestinal cell proliferation, protein absorption and metabolism of grass carp in vitro and in vivo experiments. And we studied the effects of different protein sources on the expression of Ghrelin, PepT1 and mTOR in grass carp at individual level. The results suggested that Ghrelin can promote grass carp intestinal cell proliferation, and affect the expression of genes which are related to intestinal peptide absorption and metabolism in juvenile grass carp.Yonghua ZhouMeixing GuoYingjie LiYu ZhangWenqian XuDafang ZhaoYuan ChenAnli ZuoFufa QuJianzhou TangShenping CaoZhen LiuElsevierarticleCtenopharyngodon idellaGhrelinIntestinal cell proliferationAbsorptionMetabolismAquaculture. Fisheries. AnglingSH1-691ENAquaculture Reports, Vol 22, Iss , Pp 100928- (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Ctenopharyngodon idella Ghrelin Intestinal cell proliferation Absorption Metabolism Aquaculture. Fisheries. Angling SH1-691
spellingShingle	Ctenopharyngodon idella Ghrelin Intestinal cell proliferation Absorption Metabolism Aquaculture. Fisheries. Angling SH1-691 Yonghua Zhou Meixing Guo Yingjie Li Yu Zhang Wenqian Xu Dafang Zhao Yuan Chen Anli Zuo Fufa Qu Jianzhou Tang Shenping Cao Zhen Liu Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)
description	Ghrelin can stimulate pituitary growth hormone (GH) release, and has a key role in the regulation of food intake and adiposity in vertebrates. At present, no relevant studies have reported the effects of Ghrelin on intestinal cell proliferation, protein absorption and metabolism. In this paper, firstly we established the intestinal epithelial cell lines of juvenile grass carp (Ctenopharyngodon idella) that can be passed stably and identified by the intestinal specific expression proteins E-cadherin and ZO-1. And we used this cell line as a model to investigate the effects of Ghrelin, Ala-Gln, Ala-Gln+Ghrelin on the proliferation of juvenile grass carp intestinal cells. We found that the use of Ghrelin, Ala-Gln and Ala-Gln+Ghrelin could promote the proliferation of intestinal cells of juvenile grass carp, but Ala-Gln+Ghrelin had the strongest promoting effect. Further, we investigated the effects of Ghrelin on the expression of cdx2, PepT1 and mTOR which are related to intestinal cell proliferation, protein absorption and metabolism of grass carp in vitro and in vivo experiments. And we studied the effects of different protein sources on the expression of Ghrelin, PepT1 and mTOR in grass carp at individual level. The results suggested that Ghrelin can promote grass carp intestinal cell proliferation, and affect the expression of genes which are related to intestinal peptide absorption and metabolism in juvenile grass carp.
format	article
author	Yonghua Zhou Meixing Guo Yingjie Li Yu Zhang Wenqian Xu Dafang Zhao Yuan Chen Anli Zuo Fufa Qu Jianzhou Tang Shenping Cao Zhen Liu
author_facet	Yonghua Zhou Meixing Guo Yingjie Li Yu Zhang Wenqian Xu Dafang Zhao Yuan Chen Anli Zuo Fufa Qu Jianzhou Tang Shenping Cao Zhen Liu
author_sort	Yonghua Zhou
title	Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)
title_short	Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)
title_full	Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)
title_fullStr	Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)
title_full_unstemmed	Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)
title_sort	effects of ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (ctenopharyngodon idella)
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/ee173f2aa1ba4c0a90191f94b748a6a0
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Effects of Ghrelin on intestinal cell proliferation, the expression of protein absorption and metabolism factors in juvenile grass carp (Ctenopharyngodon idella)

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