Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting

Bacteria possess a diverse collection of CRISPR-Cas systems but many do not function in mammalian cells, precluding their use in genome editing. Here the authors show that by manipulating local chromatin structures, inhibited Cas9s can have their activity restored in human cells.

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Autores principales:	Fuqiang Chen, Xiao Ding, Yongmei Feng, Timothy Seebeck, Yanfang Jiang, Gregory D. Davis
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ee1963f9ae8643bcaf0eb11a40952e78
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spelling	oai:doaj.org-article:ee1963f9ae8643bcaf0eb11a40952e782021-12-02T14:40:45ZTargeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting10.1038/ncomms149582041-1723https://doaj.org/article/ee1963f9ae8643bcaf0eb11a40952e782017-04-01T00:00:00Zhttps://doi.org/10.1038/ncomms14958https://doaj.org/toc/2041-1723Bacteria possess a diverse collection of CRISPR-Cas systems but many do not function in mammalian cells, precluding their use in genome editing. Here the authors show that by manipulating local chromatin structures, inhibited Cas9s can have their activity restored in human cells.Fuqiang ChenXiao DingYongmei FengTimothy SeebeckYanfang JiangGregory D. DavisNature PortfolioarticleScienceQENNature Communications, Vol 8, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Fuqiang Chen Xiao Ding Yongmei Feng Timothy Seebeck Yanfang Jiang Gregory D. Davis Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
description	Bacteria possess a diverse collection of CRISPR-Cas systems but many do not function in mammalian cells, precluding their use in genome editing. Here the authors show that by manipulating local chromatin structures, inhibited Cas9s can have their activity restored in human cells.
format	article
author	Fuqiang Chen Xiao Ding Yongmei Feng Timothy Seebeck Yanfang Jiang Gregory D. Davis
author_facet	Fuqiang Chen Xiao Ding Yongmei Feng Timothy Seebeck Yanfang Jiang Gregory D. Davis
author_sort	Fuqiang Chen
title	Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
title_short	Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
title_full	Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
title_fullStr	Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
title_full_unstemmed	Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting
title_sort	targeted activation of diverse crispr-cas systems for mammalian genome editing via proximal crispr targeting
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ee1963f9ae8643bcaf0eb11a40952e78
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Targeted activation of diverse CRISPR-Cas systems for mammalian genome editing via proximal CRISPR targeting

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