High dispersity of carbon nanotubes diminishes immunotoxicity in spleen

Soyoung Lee,1 Dongwoo Khang,2 Sang-Hyun Kim1 1CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 2Department of Molecular Medicine, Graduate School of Medicine, Gachon University, Incheon, Republic of Korea Background: From the...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Lee S, Khang D, Kim SH
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2015
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ee1b63fcf6ec40ed80211783272cc000
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee1b63fcf6ec40ed80211783272cc000
record_format	dspace
spelling	oai:doaj.org-article:ee1b63fcf6ec40ed80211783272cc0002021-12-02T00:07:18ZHigh dispersity of carbon nanotubes diminishes immunotoxicity in spleen1178-2013https://doaj.org/article/ee1b63fcf6ec40ed80211783272cc0002015-04-01T00:00:00Zhttp://www.dovepress.com/high-dispersity-of-carbon-nanotubes-diminishes-immunotoxicity-in-splee-peer-reviewed-article-IJNhttps://doaj.org/toc/1178-2013 Soyoung Lee,1 Dongwoo Khang,2 Sang-Hyun Kim1 1CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 2Department of Molecular Medicine, Graduate School of Medicine, Gachon University, Incheon, Republic of Korea Background: From the various physiochemical material properties, the chemical functionalization order of single-walled carbon nanotubes (swCNTs) has not been considered as a critical factor for modulating immunological responses and toxicological aspects in drug delivery applications. Although most nanomaterials, including carbon nanotubes, are specifically accumulated in spleen, few studies have focused on spleen immunotoxicity. For this reason, this study demonstrated that the dispersity of swCNTs significantly influenced immunotoxicity in vitro and in vivo. Materials and methods: For cytotoxicity of swCNTs, MTT assay, reactive oxygen species production, superoxide dismutase activity, cellular uptake, and confocal microscopy were used in macrophages. In the in vivo study, female BALB/c mice were intravenously administered with 1 mg/kg/day of swCNTs for 2 weeks. The body weight, organ weight, hematological change, reverse-transcription polymerase chain reaction, and lymphocyte population were evaluated. Results: Different orders of chemical functionalization of swCNTs controlled immunotoxicity. In short, less-dispersed swCNTs caused cytotoxicity in macrophages and abnormalities in immune organs such as spleen, whereas highly dispersed swCNTs did not result in immunotoxicity. Conclusion: This study clarified that increasing carboxyl groups on swCNTs significantly mitigated immunotoxicity in vitro and in vivo. Our findings clarified the effective immunotoxicological factors of swCNTs by increasing dispersity of swCNTs and provided useful guidelines for the effective use of nanomaterials. Keywords: carbon nanotubes, immunotoxicity, dispersity, physicochemical propertyLee SKhang DKim SHDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2015, Iss default, Pp 2697-2710 (2015)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Lee S Khang D Kim SH High dispersity of carbon nanotubes diminishes immunotoxicity in spleen
description	Soyoung Lee,1 Dongwoo Khang,2 Sang-Hyun Kim1 1CMRI, Department of Pharmacology, School of Medicine, Kyungpook National University, Daegu, Republic of Korea; 2Department of Molecular Medicine, Graduate School of Medicine, Gachon University, Incheon, Republic of Korea Background: From the various physiochemical material properties, the chemical functionalization order of single-walled carbon nanotubes (swCNTs) has not been considered as a critical factor for modulating immunological responses and toxicological aspects in drug delivery applications. Although most nanomaterials, including carbon nanotubes, are specifically accumulated in spleen, few studies have focused on spleen immunotoxicity. For this reason, this study demonstrated that the dispersity of swCNTs significantly influenced immunotoxicity in vitro and in vivo. Materials and methods: For cytotoxicity of swCNTs, MTT assay, reactive oxygen species production, superoxide dismutase activity, cellular uptake, and confocal microscopy were used in macrophages. In the in vivo study, female BALB/c mice were intravenously administered with 1 mg/kg/day of swCNTs for 2 weeks. The body weight, organ weight, hematological change, reverse-transcription polymerase chain reaction, and lymphocyte population were evaluated. Results: Different orders of chemical functionalization of swCNTs controlled immunotoxicity. In short, less-dispersed swCNTs caused cytotoxicity in macrophages and abnormalities in immune organs such as spleen, whereas highly dispersed swCNTs did not result in immunotoxicity. Conclusion: This study clarified that increasing carboxyl groups on swCNTs significantly mitigated immunotoxicity in vitro and in vivo. Our findings clarified the effective immunotoxicological factors of swCNTs by increasing dispersity of swCNTs and provided useful guidelines for the effective use of nanomaterials. Keywords: carbon nanotubes, immunotoxicity, dispersity, physicochemical property
format	article
author	Lee S Khang D Kim SH
author_facet	Lee S Khang D Kim SH
author_sort	Lee S
title	High dispersity of carbon nanotubes diminishes immunotoxicity in spleen
title_short	High dispersity of carbon nanotubes diminishes immunotoxicity in spleen
title_full	High dispersity of carbon nanotubes diminishes immunotoxicity in spleen
title_fullStr	High dispersity of carbon nanotubes diminishes immunotoxicity in spleen
title_full_unstemmed	High dispersity of carbon nanotubes diminishes immunotoxicity in spleen
title_sort	high dispersity of carbon nanotubes diminishes immunotoxicity in spleen
publisher	Dove Medical Press
publishDate	2015
url	https://doaj.org/article/ee1b63fcf6ec40ed80211783272cc000
work_keys_str_mv	AT lees highdispersityofcarbonnanotubesdiminishesimmunotoxicityinspleen AT khangd highdispersityofcarbonnanotubesdiminishesimmunotoxicityinspleen AT kimsh highdispersityofcarbonnanotubesdiminishesimmunotoxicityinspleen
_version_	1718403960003362816

High dispersity of carbon nanotubes diminishes immunotoxicity in spleen

Ejemplares similares