Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment

Abstract Orchestration of bone repair processes requires crosstalk between different cell populations, including immune cells and mesenchymal stem/stromal cells (MSC). Extracellular vesicles (EV) as mediators of these interactions remain vastly unexplored. Here, we aimed to determine the mechanism o...

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Autores principales:	Andreia M. Silva, Maria I. Almeida, José H. Teixeira, André F. Maia, George A. Calin, Mário A. Barbosa, Susana G. Santos
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee1bd1d9864744f281340bc5184ca1d6
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spelling	oai:doaj.org-article:ee1bd1d9864744f281340bc5184ca1d62021-12-02T12:32:52ZDendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment10.1038/s41598-017-01809-x2045-2322https://doaj.org/article/ee1bd1d9864744f281340bc5184ca1d62017-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-01809-xhttps://doaj.org/toc/2045-2322Abstract Orchestration of bone repair processes requires crosstalk between different cell populations, including immune cells and mesenchymal stem/stromal cells (MSC). Extracellular vesicles (EV) as mediators of these interactions remain vastly unexplored. Here, we aimed to determine the mechanism of MSC recruitment by Dendritic Cells (DC), hypothesising that it would be mediated by EV. Primary human DC-secreted EV (DC-EV), isolated by ultracentrifugation, were characterized for their size, morphology and protein markers, indicating an enrichment in exosomes. DC-EV were readily internalized by human bone marrow-derived MSC, without impacting significantly their proliferation or influencing their osteogenic/chondrogenic differentiation. Importantly, DC-EV significantly and dose-dependently promoted MSC recruitment across a transwell system and enhanced MSC migration in a microfluidic chemotaxis assay. DC-EV content was analysed by chemokine array, indicating the presence of chemotactic mediators. Osteopontin and matrix metalloproteinase-9 were confirmed inside EV. In summary, DC-EV are naturally loaded with chemoattractants and can contribute to cell recruitment, thus inspiring the development of new tissue regeneration strategies.Andreia M. SilvaMaria I. AlmeidaJosé H. TeixeiraAndré F. MaiaGeorge A. CalinMário A. BarbosaSusana G. SantosNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-15 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Andreia M. Silva Maria I. Almeida José H. Teixeira André F. Maia George A. Calin Mário A. Barbosa Susana G. Santos Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment
description	Abstract Orchestration of bone repair processes requires crosstalk between different cell populations, including immune cells and mesenchymal stem/stromal cells (MSC). Extracellular vesicles (EV) as mediators of these interactions remain vastly unexplored. Here, we aimed to determine the mechanism of MSC recruitment by Dendritic Cells (DC), hypothesising that it would be mediated by EV. Primary human DC-secreted EV (DC-EV), isolated by ultracentrifugation, were characterized for their size, morphology and protein markers, indicating an enrichment in exosomes. DC-EV were readily internalized by human bone marrow-derived MSC, without impacting significantly their proliferation or influencing their osteogenic/chondrogenic differentiation. Importantly, DC-EV significantly and dose-dependently promoted MSC recruitment across a transwell system and enhanced MSC migration in a microfluidic chemotaxis assay. DC-EV content was analysed by chemokine array, indicating the presence of chemotactic mediators. Osteopontin and matrix metalloproteinase-9 were confirmed inside EV. In summary, DC-EV are naturally loaded with chemoattractants and can contribute to cell recruitment, thus inspiring the development of new tissue regeneration strategies.
format	article
author	Andreia M. Silva Maria I. Almeida José H. Teixeira André F. Maia George A. Calin Mário A. Barbosa Susana G. Santos
author_facet	Andreia M. Silva Maria I. Almeida José H. Teixeira André F. Maia George A. Calin Mário A. Barbosa Susana G. Santos
author_sort	Andreia M. Silva
title	Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment
title_short	Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment
title_full	Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment
title_fullStr	Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment
title_full_unstemmed	Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment
title_sort	dendritic cell-derived extracellular vesicles mediate mesenchymal stem/stromal cell recruitment
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ee1bd1d9864744f281340bc5184ca1d6
work_keys_str_mv	AT andreiamsilva dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment AT mariaialmeida dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment AT josehteixeira dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment AT andrefmaia dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment AT georgeacalin dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment AT marioabarbosa dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment AT susanagsantos dendriticcellderivedextracellularvesiclesmediatemesenchymalstemstromalcellrecruitment
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Dendritic Cell-derived Extracellular Vesicles mediate Mesenchymal Stem/Stromal Cell recruitment

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