Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.

Roots of intact olive seedlings, axenically cultured, were alternatively placed in contact with Rhizophagus irregularis (mycorrhizal) or Verticillim dahliae (pathogenic) fungi. MeJA treatments were also included. In vivo redox activities in the apoplast of the intact roots (anion superoxide generati...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Francisco Espinosa, Inmaculada Garrido, Alfonso Ortega, Ilda Casimiro, Ma Carmen Álvarez-Tinaut
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee1c532f10594170bd96ae3dd7c271f6
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee1c532f10594170bd96ae3dd7c271f6
record_format	dspace
spelling	oai:doaj.org-article:ee1c532f10594170bd96ae3dd7c271f62021-11-11T08:21:27ZRedox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.1932-620310.1371/journal.pone.0100132https://doaj.org/article/ee1c532f10594170bd96ae3dd7c271f62014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24967716/?tool=EBIhttps://doaj.org/toc/1932-6203Roots of intact olive seedlings, axenically cultured, were alternatively placed in contact with Rhizophagus irregularis (mycorrhizal) or Verticillim dahliae (pathogenic) fungi. MeJA treatments were also included. In vivo redox activities in the apoplast of the intact roots (anion superoxide generation, superoxide dismutase and peroxidase activities) were measured. All our results showed that apoplastic redox activities of intact seedling roots in contact with the compatible mycorrhizal fungus were clearly attenuated in comparison with the pathogenic fungus or treated with MeJA, even at the early stages of treatment used. Total phenolics, flavonoids and phenylpropanoid glycosides were also quantified. Roots in contact with the mycorrhizal fungus did not enhance the biosynthesis of phenolic compounds with respect to controls, while those in contact with the pathogenic one significantly enhanced the biosynthesis of all phenolic fractions measured. Reactive oxygen species and nitric oxid accumulation in roots were examined by fluorescence microscopy. All of them presented much higher accumulation in roots in contact with the pathogenic than with the mycorrhizal fungus. Altogether these results indicate that intact olive seedling roots clearly differentiated between mycorrhizal and pathogenic fungi, attenuating defense reactions against the first to facilitate its establishment, while inducing a strong and sustained defense reaction against the second. Both reactive oxygen and nitrogen species seemed to be involved in these responses from the first moments of contact. However, further investigations are required to clarify the proposed crosstalk between them and their respective roles in these responses since fluorescence images of roots revealed that reactive oxygen species were mainly accumulated in the apoplast (congruently with the measured redox activities in this compartment) while nitric oxid was mainly stored in the cytosol.Francisco EspinosaInmaculada GarridoAlfonso OrtegaIlda CasimiroMa Carmen Álvarez-TinautPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 6, p e100132 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Francisco Espinosa Inmaculada Garrido Alfonso Ortega Ilda Casimiro Ma Carmen Álvarez-Tinaut Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
description	Roots of intact olive seedlings, axenically cultured, were alternatively placed in contact with Rhizophagus irregularis (mycorrhizal) or Verticillim dahliae (pathogenic) fungi. MeJA treatments were also included. In vivo redox activities in the apoplast of the intact roots (anion superoxide generation, superoxide dismutase and peroxidase activities) were measured. All our results showed that apoplastic redox activities of intact seedling roots in contact with the compatible mycorrhizal fungus were clearly attenuated in comparison with the pathogenic fungus or treated with MeJA, even at the early stages of treatment used. Total phenolics, flavonoids and phenylpropanoid glycosides were also quantified. Roots in contact with the mycorrhizal fungus did not enhance the biosynthesis of phenolic compounds with respect to controls, while those in contact with the pathogenic one significantly enhanced the biosynthesis of all phenolic fractions measured. Reactive oxygen species and nitric oxid accumulation in roots were examined by fluorescence microscopy. All of them presented much higher accumulation in roots in contact with the pathogenic than with the mycorrhizal fungus. Altogether these results indicate that intact olive seedling roots clearly differentiated between mycorrhizal and pathogenic fungi, attenuating defense reactions against the first to facilitate its establishment, while inducing a strong and sustained defense reaction against the second. Both reactive oxygen and nitrogen species seemed to be involved in these responses from the first moments of contact. However, further investigations are required to clarify the proposed crosstalk between them and their respective roles in these responses since fluorescence images of roots revealed that reactive oxygen species were mainly accumulated in the apoplast (congruently with the measured redox activities in this compartment) while nitric oxid was mainly stored in the cytosol.
format	article
author	Francisco Espinosa Inmaculada Garrido Alfonso Ortega Ilda Casimiro Ma Carmen Álvarez-Tinaut
author_facet	Francisco Espinosa Inmaculada Garrido Alfonso Ortega Ilda Casimiro Ma Carmen Álvarez-Tinaut
author_sort	Francisco Espinosa
title	Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
title_short	Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
title_full	Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
title_fullStr	Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
title_full_unstemmed	Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
title_sort	redox activities and ros, no and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ee1c532f10594170bd96ae3dd7c271f6
work_keys_str_mv	AT franciscoespinosa redoxactivitiesandrosnoandphenylpropanoidsproductionbyaxenicallyculturedintactoliveseedlingrootsafterinteractionwithamycorrhizalorapathogenicfungus AT inmaculadagarrido redoxactivitiesandrosnoandphenylpropanoidsproductionbyaxenicallyculturedintactoliveseedlingrootsafterinteractionwithamycorrhizalorapathogenicfungus AT alfonsoortega redoxactivitiesandrosnoandphenylpropanoidsproductionbyaxenicallyculturedintactoliveseedlingrootsafterinteractionwithamycorrhizalorapathogenicfungus AT ildacasimiro redoxactivitiesandrosnoandphenylpropanoidsproductionbyaxenicallyculturedintactoliveseedlingrootsafterinteractionwithamycorrhizalorapathogenicfungus AT macarmenalvareztinaut redoxactivitiesandrosnoandphenylpropanoidsproductionbyaxenicallyculturedintactoliveseedlingrootsafterinteractionwithamycorrhizalorapathogenicfungus
_version_	1718439362072412160

Redox activities and ROS, NO and phenylpropanoids production by axenically cultured intact olive seedling roots after interaction with a mycorrhizal or a pathogenic fungus.

Ejemplares similares