Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples

An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-rea...

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Autores principales:	Daniel Mukunzi, Steven Suryoprabowo, Shanshan Song, Liqiang Liu, Hua Kuang
Formato:	article
Lenguaje:	EN
Publicado:	Taylor & Francis Group 2018
Materias:	pefloxacin monoclonal antibody indirect competitive enzyme-linked immunosorbent assay lateral flow strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ee1d7fcf872c4e8b85551d0964637079
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spelling	oai:doaj.org-article:ee1d7fcf872c4e8b85551d09646370792021-11-26T11:19:46ZDevelopment of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples0954-01051465-344310.1080/09540105.2017.1406460https://doaj.org/article/ee1d7fcf872c4e8b85551d09646370792018-01-01T00:00:00Zhttp://dx.doi.org/10.1080/09540105.2017.1406460https://doaj.org/toc/0954-0105https://doaj.org/toc/1465-3443An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-reactivity with nine FQs with a limit of detection of 0.082 ng/mL assayed in 0.01 M phosphate buffered saline (PBS) solution. The intra- and inter-assay recoveries from spiked samples were within the range of 62.42–111.47% and 63.5–113.79%, respectively. The visual cut-off values of the lateral flow test strip in 0.01 M PBS and in food matrices were within the range of 2.5–50 ng/mL and 5–100 µg/kg, respectively. These results show that the anti-PEF mAb immunoassay and lateral flow test strip methods are suitable for simultaneous detection and routine monitoring of FQ residues in food.Daniel MukunziSteven SuryoprabowoShanshan SongLiqiang LiuHua KuangTaylor & Francis Grouparticlepefloxacinmonoclonal antibodyindirect competitive enzyme-linked immunosorbent assaylateral flowstripAgriculture (General)S1-972Immunologic diseases. AllergyRC581-607ENFood and Agricultural Immunology, Vol 29, Iss 1, Pp 484-497 (2018)
institution	DOAJ
collection	DOAJ
language	EN
topic	pefloxacin monoclonal antibody indirect competitive enzyme-linked immunosorbent assay lateral flow strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607
spellingShingle	pefloxacin monoclonal antibody indirect competitive enzyme-linked immunosorbent assay lateral flow strip Agriculture (General) S1-972 Immunologic diseases. Allergy RC581-607 Daniel Mukunzi Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
description	An anti-pefloxacin (PEF) monoclonal antibody (mAb), an indirect competitive enzyme-linked immunosorbent assay and lateral-flow test strip methods were developed to detect fluoroquinolone (FQ) residues in chicken muscle samples. Under optimised conditions, the anti-PEF mAb showed reasonable cross-reactivity with nine FQs with a limit of detection of 0.082 ng/mL assayed in 0.01 M phosphate buffered saline (PBS) solution. The intra- and inter-assay recoveries from spiked samples were within the range of 62.42–111.47% and 63.5–113.79%, respectively. The visual cut-off values of the lateral flow test strip in 0.01 M PBS and in food matrices were within the range of 2.5–50 ng/mL and 5–100 µg/kg, respectively. These results show that the anti-PEF mAb immunoassay and lateral flow test strip methods are suitable for simultaneous detection and routine monitoring of FQ residues in food.
format	article
author	Daniel Mukunzi Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang
author_facet	Daniel Mukunzi Steven Suryoprabowo Shanshan Song Liqiang Liu Hua Kuang
author_sort	Daniel Mukunzi
title	Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
title_short	Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
title_full	Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
title_fullStr	Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
title_full_unstemmed	Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
title_sort	development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples
publisher	Taylor & Francis Group
publishDate	2018
url	https://doaj.org/article/ee1d7fcf872c4e8b85551d0964637079
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Development of an indirect enzyme-linked immunosorbent assay and lateral-flow test strips for pefloxacin and its analogues in chicken muscle samples

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