Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.

Fusarium is the major causative agent of fungal infections leading to corneal ulcer (keratitis) in Southern India and other tropical countries. Keratitis caused by Fusarium is a difficult disease to treat unless antifungal therapy is initiated during the early stages of infection. In this study tear...

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Autores principales:	Sivagnanam Ananthi, Namperumalsamy Venkatesh Prajna, Prajna Lalitha, Murugesan Valarnila, Kuppamuthu Dharmalingam
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee21de34fcff4f80adc28dcd28c101a7
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spelling	oai:doaj.org-article:ee21de34fcff4f80adc28dcd28c101a72021-11-18T08:02:23ZPathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.1932-620310.1371/journal.pone.0053018https://doaj.org/article/ee21de34fcff4f80adc28dcd28c101a72013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23308132/pdf/?tool=EBIhttps://doaj.org/toc/1932-6203Fusarium is the major causative agent of fungal infections leading to corneal ulcer (keratitis) in Southern India and other tropical countries. Keratitis caused by Fusarium is a difficult disease to treat unless antifungal therapy is initiated during the early stages of infection. In this study tear proteins were prepared from keratitis patients classified based on the duration of infection. Among the patients recruited, early infection (n = 35), intermediate (n = 20), late (n = 11), samples from five patients in each group were pooled for analysis. Control samples were a pool of samples from 20 patients. Proteins were separated on difference gel electrophoresis (DIGE) and the differentially expressed proteins were quantified using DeCyder software analysis. The following differentially expressed proteins namely alpha-1-antitrypsin, haptoglobin α2 chain, zinc-alpha-2-glycoprotein, apolipoprotein, albumin, haptoglobin precursor - β chain, lactoferrin, lacrimal lipocalin precursor, cystatin SA III precursor, lacritin precursor were identified using mass spectrometry. Variation in the expression level of some of the proteins was confirmed using western blot analysis. This is the first report to show stage specific tear protein profile in fungal keratitis patients. Validation of this data using a much larger sample set could lead to clinical application of these findings.Sivagnanam AnanthiNamperumalsamy Venkatesh PrajnaPrajna LalithaMurugesan ValarnilaKuppamuthu DharmalingamPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 1, p e53018 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sivagnanam Ananthi Namperumalsamy Venkatesh Prajna Prajna Lalitha Murugesan Valarnila Kuppamuthu Dharmalingam Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.
description	Fusarium is the major causative agent of fungal infections leading to corneal ulcer (keratitis) in Southern India and other tropical countries. Keratitis caused by Fusarium is a difficult disease to treat unless antifungal therapy is initiated during the early stages of infection. In this study tear proteins were prepared from keratitis patients classified based on the duration of infection. Among the patients recruited, early infection (n = 35), intermediate (n = 20), late (n = 11), samples from five patients in each group were pooled for analysis. Control samples were a pool of samples from 20 patients. Proteins were separated on difference gel electrophoresis (DIGE) and the differentially expressed proteins were quantified using DeCyder software analysis. The following differentially expressed proteins namely alpha-1-antitrypsin, haptoglobin α2 chain, zinc-alpha-2-glycoprotein, apolipoprotein, albumin, haptoglobin precursor - β chain, lactoferrin, lacrimal lipocalin precursor, cystatin SA III precursor, lacritin precursor were identified using mass spectrometry. Variation in the expression level of some of the proteins was confirmed using western blot analysis. This is the first report to show stage specific tear protein profile in fungal keratitis patients. Validation of this data using a much larger sample set could lead to clinical application of these findings.
format	article
author	Sivagnanam Ananthi Namperumalsamy Venkatesh Prajna Prajna Lalitha Murugesan Valarnila Kuppamuthu Dharmalingam
author_facet	Sivagnanam Ananthi Namperumalsamy Venkatesh Prajna Prajna Lalitha Murugesan Valarnila Kuppamuthu Dharmalingam
author_sort	Sivagnanam Ananthi
title	Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.
title_short	Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.
title_full	Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.
title_fullStr	Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.
title_full_unstemmed	Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.
title_sort	pathogen induced changes in the protein profile of human tears from fusarium keratitis patients.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/ee21de34fcff4f80adc28dcd28c101a7
work_keys_str_mv	AT sivagnanamananthi pathogeninducedchangesintheproteinprofileofhumantearsfromfusariumkeratitispatients AT namperumalsamyvenkateshprajna pathogeninducedchangesintheproteinprofileofhumantearsfromfusariumkeratitispatients AT prajnalalitha pathogeninducedchangesintheproteinprofileofhumantearsfromfusariumkeratitispatients AT murugesanvalarnila pathogeninducedchangesintheproteinprofileofhumantearsfromfusariumkeratitispatients AT kuppamuthudharmalingam pathogeninducedchangesintheproteinprofileofhumantearsfromfusariumkeratitispatients
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Pathogen induced changes in the protein profile of human tears from Fusarium keratitis patients.

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