СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS

With the help of molecular genetic method we have investigated the level of mRNA gene expressions Glut1, mTOR and AMPK1α in PLN in pancreatic lymph nodes (PLN) of rats with streptozotocininduced diabetes mellitus (SIDM) and after administration of metformin. The levels of Glut1, mTOR and AMPK1α mRNA...

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Autores principales:	D. A. Putilin, A. M. Kamyshnyi
Formato:	article
Lenguaje:	RU
Publicado:	SPb RAACI 2016
Materias:	streptozotocin-induced diabetes mellitus lymph nodes pancreatic glut1 mtor ampk1α gene expression Immunologic diseases. Allergy RC581-607
Acceso en línea:	https://doaj.org/article/ee23b1fa141144b09e299d704b3b001c
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spelling	oai:doaj.org-article:ee23b1fa141144b09e299d704b3b001c2021-11-18T08:03:45ZСHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS1563-06252313-741X10.15789/1563-0625-2016-4-339-346https://doaj.org/article/ee23b1fa141144b09e299d704b3b001c2016-08-01T00:00:00Zhttps://www.mimmun.ru/mimmun/article/view/1056https://doaj.org/toc/1563-0625https://doaj.org/toc/2313-741XWith the help of molecular genetic method we have investigated the level of mRNA gene expressions Glut1, mTOR and AMPK1α in PLN in pancreatic lymph nodes (PLN) of rats with streptozotocininduced diabetes mellitus (SIDM) and after administration of metformin. The levels of Glut1, mTOR and AMPK1α mRNA were determined by means of RT-PCR using CFX96™ thermocycler (Real-Time PCR Detection Systems, Bio-Rad, USA). Relative gene expression levels were calculated as ratios to GAPDH reference gene using ΔΔCt method. Statistical analysis was performed with available “Bio-Rad СFX Manager 3.1” software (Bio-Rad, USA). The mTOR+ positive lymphocytes were identified by means of monoclonal antibodies, using an indirect immunofluorescence method. Hyperglycemia was accompanied by transcriptional induction of glucose transporter Glut1 gene (9.9 to 28.9-fold, p < 0.05), and mTOR protein kinase gene (5.3 to 3.3-fold, p < 0.05) in PLN. Development of diabetes was also associated with increase by 24-34% in total mTOR+ cell numbers in PLN at the 5th week of developing diabetes (p < 0.05) and increased concentrations of rapamycin target in the immunopositive cells. Metformin administration to diabetic rats was followed by increased AMPK1α mRNA level of by 87% (p < 0,05) at the 3rd week, and 38-fold (p < 0,05), at the 5th week of SIDM development and inhibition of mTOR expression in PLN (3 to 14.7-fold, p < 0.05) accompanied by a 40 per cent decrease (p < 0.05) in total density of mTOR+ cells in PLN lymph cords of the rats following 5 weeks of SIDM.D. A. PutilinA. M. KamyshnyiSPb RAACIarticlestreptozotocin-induced diabetes mellituslymph nodespancreaticglut1mtorampk1αgene expressionImmunologic diseases. AllergyRC581-607RUMedicinskaâ Immunologiâ, Vol 18, Iss 4, Pp 339-346 (2016)
institution	DOAJ
collection	DOAJ
language	RU
topic	streptozotocin-induced diabetes mellitus lymph nodes pancreatic glut1 mtor ampk1α gene expression Immunologic diseases. Allergy RC581-607
spellingShingle	streptozotocin-induced diabetes mellitus lymph nodes pancreatic glut1 mtor ampk1α gene expression Immunologic diseases. Allergy RC581-607 D. A. Putilin A. M. Kamyshnyi СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
description	With the help of molecular genetic method we have investigated the level of mRNA gene expressions Glut1, mTOR and AMPK1α in PLN in pancreatic lymph nodes (PLN) of rats with streptozotocininduced diabetes mellitus (SIDM) and after administration of metformin. The levels of Glut1, mTOR and AMPK1α mRNA were determined by means of RT-PCR using CFX96™ thermocycler (Real-Time PCR Detection Systems, Bio-Rad, USA). Relative gene expression levels were calculated as ratios to GAPDH reference gene using ΔΔCt method. Statistical analysis was performed with available “Bio-Rad СFX Manager 3.1” software (Bio-Rad, USA). The mTOR+ positive lymphocytes were identified by means of monoclonal antibodies, using an indirect immunofluorescence method. Hyperglycemia was accompanied by transcriptional induction of glucose transporter Glut1 gene (9.9 to 28.9-fold, p < 0.05), and mTOR protein kinase gene (5.3 to 3.3-fold, p < 0.05) in PLN. Development of diabetes was also associated with increase by 24-34% in total mTOR+ cell numbers in PLN at the 5th week of developing diabetes (p < 0.05) and increased concentrations of rapamycin target in the immunopositive cells. Metformin administration to diabetic rats was followed by increased AMPK1α mRNA level of by 87% (p < 0,05) at the 3rd week, and 38-fold (p < 0,05), at the 5th week of SIDM development and inhibition of mTOR expression in PLN (3 to 14.7-fold, p < 0.05) accompanied by a 40 per cent decrease (p < 0.05) in total density of mTOR+ cells in PLN lymph cords of the rats following 5 weeks of SIDM.
format	article
author	D. A. Putilin A. M. Kamyshnyi
author_facet	D. A. Putilin A. M. Kamyshnyi
author_sort	D. A. Putilin
title	СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
title_short	СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
title_full	СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
title_fullStr	СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
title_full_unstemmed	СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS
title_sort	сhanges of glut1, mtor and ampk1α gene expression in pancreatic lymph node lymphocytes of rats with experimental diabetes mellitus
publisher	SPb RAACI
publishDate	2016
url	https://doaj.org/article/ee23b1fa141144b09e299d704b3b001c
work_keys_str_mv	AT daputilin shangesofglut1mtorandampk1ageneexpressioninpancreaticlymphnodelymphocytesofratswithexperimentaldiabetesmellitus AT amkamyshnyi shangesofglut1mtorandampk1ageneexpressioninpancreaticlymphnodelymphocytesofratswithexperimentaldiabetesmellitus
_version_	1718422454922117120

СHANGES OF Glut1, mTOR AND AMPK1α GENE EXPRESSION IN PANCREATIC LYMPH NODE LYMPHOCYTES OF RATS WITH EXPERIMENTAL DIABETES MELLITUS

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