Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs

This study aimed to search the extent of spread of various canine parvovirus variants in Egypt from year 2019 to year 2020 and the ability of the present used vaccines in the protection .In this study, twenty fecal samples were collected from diarrheic puppies. These samples were tested by rapid CPV...

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Autores principales:	Morcos I.Yanni, Ebtsam,A.Abouelyazeed, Nadia Maher Hanna, Hala K. Abdelmegeed, Omnia M. Khattab
Formato:	article
Lenguaje:	EN
Publicado:	Egyptian Society for Animal Management 2021
Materias:	canine parvovirus dog egypt pcr sequencing Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
Acceso en línea:	https://dx.doi.org/10.21608/javs.2021.78123.1082 https://doaj.org/article/ee23bd77174743b08732111ef193e14c
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spelling	oai:doaj.org-article:ee23bd77174743b08732111ef193e14c2021-12-02T15:32:50ZVirologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogshttps://dx.doi.org/10.21608/javs.2021.78123.10821687-40722090-3308https://doaj.org/article/ee23bd77174743b08732111ef193e14c2021-07-01T00:00:00Zhttps://javs.journals.ekb.eg/article_180535.htmlhttps://doaj.org/toc/1687-4072https://doaj.org/toc/2090-3308This study aimed to search the extent of spread of various canine parvovirus variants in Egypt from year 2019 to year 2020 and the ability of the present used vaccines in the protection .In this study, twenty fecal samples were collected from diarrheic puppies. These samples were tested by rapid CPV Ag test kits for the detection of CPV-2 Ag. Ten samples were found positive for CPV2 by rapid Ag test kit (VDRG CPV Ag rapid kit).Positive samples were propagated on Vero cell culture for 8 passages, CPE appears in the form of cell rounding and detachment within 5 days. A conventional polymerase chain reaction test was performed on tissue culture isolates. Partial sequencing of five randomly selected positive samples revealed the presence of 2 CPV2C and 3 CPV2B.CPV2B represents the highest level of the variant serotype of CPV circulating in Egypt through records of egyptian isolated cases at the gene bank . One of the isolate has 100% identity with a CPV2B of cat origin. Our isolates CPVS1 and CPVS4 have 100% nucleotide identity with each other and with CPV cairo1-19 and CPV cairo3-19 belonging to CPV2C , this variant is also widespread all over the country. Continuous accurate molecular and epidemiological studies are needed to follow-up the new mutation of the virus genome which may result in vaccination failure(The majority of the used vaccines in Egypt are of CPV2 origin not of variant origin). The use of CPV2b based vaccine gave better protection than cpv2 based vaccine(Wide range protection against CPV2 variants). The obvious spread and emergence of newly introduced CPV variants at different Egyptian governorates as cited at previous study necessitates updated molecular based epidemiological studies of field isolates continuously to follow up the efficacy of the concurrent used vaccines.Morcos I.YanniEbtsam,A.AbouelyazeedNadia Maher HannaHala K. Abdelmegeed Omnia M. KhattabEgyptian Society for Animal Managementarticlecanine parvovirusdogegyptpcrsequencingZoologyQL1-991Veterinary medicineSF600-1100Animal biochemistryQP501-801ENJournal of Applied Veterinary Sciences, Vol 6, Iss 3, Pp 48-53 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	canine parvovirus dog egypt pcr sequencing Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801
spellingShingle	canine parvovirus dog egypt pcr sequencing Zoology QL1-991 Veterinary medicine SF600-1100 Animal biochemistry QP501-801 Morcos I.Yanni Ebtsam,A.Abouelyazeed Nadia Maher Hanna Hala K. Abdelmegeed Omnia M. Khattab Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs
description	This study aimed to search the extent of spread of various canine parvovirus variants in Egypt from year 2019 to year 2020 and the ability of the present used vaccines in the protection .In this study, twenty fecal samples were collected from diarrheic puppies. These samples were tested by rapid CPV Ag test kits for the detection of CPV-2 Ag. Ten samples were found positive for CPV2 by rapid Ag test kit (VDRG CPV Ag rapid kit).Positive samples were propagated on Vero cell culture for 8 passages, CPE appears in the form of cell rounding and detachment within 5 days. A conventional polymerase chain reaction test was performed on tissue culture isolates. Partial sequencing of five randomly selected positive samples revealed the presence of 2 CPV2C and 3 CPV2B.CPV2B represents the highest level of the variant serotype of CPV circulating in Egypt through records of egyptian isolated cases at the gene bank . One of the isolate has 100% identity with a CPV2B of cat origin. Our isolates CPVS1 and CPVS4 have 100% nucleotide identity with each other and with CPV cairo1-19 and CPV cairo3-19 belonging to CPV2C , this variant is also widespread all over the country. Continuous accurate molecular and epidemiological studies are needed to follow-up the new mutation of the virus genome which may result in vaccination failure(The majority of the used vaccines in Egypt are of CPV2 origin not of variant origin). The use of CPV2b based vaccine gave better protection than cpv2 based vaccine(Wide range protection against CPV2 variants). The obvious spread and emergence of newly introduced CPV variants at different Egyptian governorates as cited at previous study necessitates updated molecular based epidemiological studies of field isolates continuously to follow up the efficacy of the concurrent used vaccines.
format	article
author	Morcos I.Yanni Ebtsam,A.Abouelyazeed Nadia Maher Hanna Hala K. Abdelmegeed Omnia M. Khattab
author_facet	Morcos I.Yanni Ebtsam,A.Abouelyazeed Nadia Maher Hanna Hala K. Abdelmegeed Omnia M. Khattab
author_sort	Morcos I.Yanni
title	Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs
title_short	Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs
title_full	Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs
title_fullStr	Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs
title_full_unstemmed	Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs
title_sort	virologic detection and molecular characterization of canine parvovirus-2 in dogs
publisher	Egyptian Society for Animal Management
publishDate	2021
url	https://dx.doi.org/10.21608/javs.2021.78123.1082 https://doaj.org/article/ee23bd77174743b08732111ef193e14c
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Virologic Detection and Molecular Characterization of Canine Parvovirus-2 in Dogs

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