Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

Hisao Haniu1, Naoto Saito2, Yoshikazu Matsuda3, Yoong-Ahm Kim4, Ki Chul Park1, Tamotsu Tsukahara5, Yuki Usui6, Kaoru Aoki7, Masayuki Shimizu7, Nobuhide Ogihara7, Kazuo Hara7, Seiji Takanashi7, Masanori Okamoto7, Norio Ishigaki7, Koichi Nakamura7, Hiroyuki Kato71Institute of Carbon Science and Techno...

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Autores principales:	Haniu H, Saito N, Matsuda Y, Kim YA, Park KC, Tsukahara T, Usui Y, Aoki K, Shimizu M, Ogihara N, Hara K, Takanashi S, Okamoto M, Ishigaki N, Nakamura K, Kato H
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2011
Materias:	Medicine (General) R5-920
Acceso en línea:	https://doaj.org/article/ee2b8a7c085f41768ad5269ea9d9a16a
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spelling	oai:doaj.org-article:ee2b8a7c085f41768ad5269ea9d9a16a2021-12-02T01:50:40ZElucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines1176-91141178-2013https://doaj.org/article/ee2b8a7c085f41768ad5269ea9d9a16a2011-12-01T00:00:00Zhttp://www.dovepress.com/elucidation-mechanism-of-different-biological-responses-to-multi-walle-a8927https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Hisao Haniu1, Naoto Saito2, Yoshikazu Matsuda3, Yoong-Ahm Kim4, Ki Chul Park1, Tamotsu Tsukahara5, Yuki Usui6, Kaoru Aoki7, Masayuki Shimizu7, Nobuhide Ogihara7, Kazuo Hara7, Seiji Takanashi7, Masanori Okamoto7, Norio Ishigaki7, Koichi Nakamura7, Hiroyuki Kato71Institute of Carbon Science and Technology, Shinshu University, Matsumoto, Nagano, Japan; 2Department of Applied Physical Therapy, Shinshu University School of Health Sciences, Matsumoto, Nagano, Japan; 3Clinical Pharmacology Educational Center, Nihon Pharmaceutical University, Ina-machi, Saitama, Japan; 4Faculty of Engineering, Shinshu University, Nagano-shi, Nagano, Japan; 5Department of Integrative Physiology and Bio-System Control, Shinshu University School of Medicine, Matsumoto-shi, Nagano, Japan; 6Research Center for Exotic Nanocarbons, Shinshu University, Matsumoto, Nagano, Japan; 7Department of Orthopaedic Surgery, Shinshu University School of Medicine, Matsumoto, Nagano, JapanAbstract: We examined differences in cellular responses to multi-walled carbon nanotubes (MWCNTs) using malignant pleural mesothelioma cells (MESO-1), bronchial epithelial cells (BEAS-2B), neuroblastoma cells (IMR-32), and monoblastic cells (THP-1), before and after differentiation. MESO-1, BEAS-2B and differentiated THP-1 cells actively endocytosed MWCNTs, resulting in cytotoxicity with lysosomal injury. However, cytotoxicity did not occur in IMR-32 or undifferentiated THP-1 cells. Both differentiated and undifferentiated THP-1 cells exhibited an inflammatory response. Carbon blacks were endocytosed by the same cell types without lysosomal damage and caused cytokine secretion, but they did not cause cytotoxicity. These results indicate that the cytotoxicity of MWCNTs requires not only cellular uptake but also lysosomal injury. Furthermore, it seems that membrane permeability or cytokine secretion without cytotoxicity results from several active mechanisms. Clarification of the cellular recognition mechanism for MWCNTs is important for developing safer MWCNTs.Keywords: multi-walled carbon nanotubes, cytotoxicity, endocytosis, cytokine secretion, reactive oxygen speciesHaniu HSaito NMatsuda YKim YAPark KCTsukahara TUsui YAoki KShimizu MOgihara NHara KTakanashi SOkamoto MIshigaki NNakamura KKato HDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2011, Iss default, Pp 3487-3497 (2011)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine (General) R5-920
spellingShingle	Medicine (General) R5-920 Haniu H Saito N Matsuda Y Kim YA Park KC Tsukahara T Usui Y Aoki K Shimizu M Ogihara N Hara K Takanashi S Okamoto M Ishigaki N Nakamura K Kato H Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
description	Hisao Haniu1, Naoto Saito2, Yoshikazu Matsuda3, Yoong-Ahm Kim4, Ki Chul Park1, Tamotsu Tsukahara5, Yuki Usui6, Kaoru Aoki7, Masayuki Shimizu7, Nobuhide Ogihara7, Kazuo Hara7, Seiji Takanashi7, Masanori Okamoto7, Norio Ishigaki7, Koichi Nakamura7, Hiroyuki Kato71Institute of Carbon Science and Technology, Shinshu University, Matsumoto, Nagano, Japan; 2Department of Applied Physical Therapy, Shinshu University School of Health Sciences, Matsumoto, Nagano, Japan; 3Clinical Pharmacology Educational Center, Nihon Pharmaceutical University, Ina-machi, Saitama, Japan; 4Faculty of Engineering, Shinshu University, Nagano-shi, Nagano, Japan; 5Department of Integrative Physiology and Bio-System Control, Shinshu University School of Medicine, Matsumoto-shi, Nagano, Japan; 6Research Center for Exotic Nanocarbons, Shinshu University, Matsumoto, Nagano, Japan; 7Department of Orthopaedic Surgery, Shinshu University School of Medicine, Matsumoto, Nagano, JapanAbstract: We examined differences in cellular responses to multi-walled carbon nanotubes (MWCNTs) using malignant pleural mesothelioma cells (MESO-1), bronchial epithelial cells (BEAS-2B), neuroblastoma cells (IMR-32), and monoblastic cells (THP-1), before and after differentiation. MESO-1, BEAS-2B and differentiated THP-1 cells actively endocytosed MWCNTs, resulting in cytotoxicity with lysosomal injury. However, cytotoxicity did not occur in IMR-32 or undifferentiated THP-1 cells. Both differentiated and undifferentiated THP-1 cells exhibited an inflammatory response. Carbon blacks were endocytosed by the same cell types without lysosomal damage and caused cytokine secretion, but they did not cause cytotoxicity. These results indicate that the cytotoxicity of MWCNTs requires not only cellular uptake but also lysosomal injury. Furthermore, it seems that membrane permeability or cytokine secretion without cytotoxicity results from several active mechanisms. Clarification of the cellular recognition mechanism for MWCNTs is important for developing safer MWCNTs.Keywords: multi-walled carbon nanotubes, cytotoxicity, endocytosis, cytokine secretion, reactive oxygen species
format	article
author	Haniu H Saito N Matsuda Y Kim YA Park KC Tsukahara T Usui Y Aoki K Shimizu M Ogihara N Hara K Takanashi S Okamoto M Ishigaki N Nakamura K Kato H
author_facet	Haniu H Saito N Matsuda Y Kim YA Park KC Tsukahara T Usui Y Aoki K Shimizu M Ogihara N Hara K Takanashi S Okamoto M Ishigaki N Nakamura K Kato H
author_sort	Haniu H
title	Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
title_short	Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
title_full	Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
title_fullStr	Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
title_full_unstemmed	Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
title_sort	elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines
publisher	Dove Medical Press
publishDate	2011
url	https://doaj.org/article/ee2b8a7c085f41768ad5269ea9d9a16a
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Elucidation mechanism of different biological responses to multi-walled carbon nanotubes using four cell lines

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