An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>

ABSTRACT A plasmid-based expression system wherein mekB was fused to a constitutive Methanosarcina acetivorans promoter was used to express MekB, a broad-specificity esterase from Pseudomonas veronii, in M. acetivorans. The engineered strain had 80-fold greater esterase activity than wild-type M. ac...

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Autores principales:	Daniel J. Lessner, Lexan Lhu, Christopher S. Wahal, James G. Ferry
Formato:	article
Lenguaje:	EN
Publicado:	American Society for Microbiology 2010
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/ee2e5de390c84b4088971e70a94e55d7
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spelling	oai:doaj.org-article:ee2e5de390c84b4088971e70a94e55d72021-11-15T15:38:17ZAn Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>10.1128/mBio.00243-102150-7511https://doaj.org/article/ee2e5de390c84b4088971e70a94e55d72010-12-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00243-10https://doaj.org/toc/2150-7511ABSTRACT A plasmid-based expression system wherein mekB was fused to a constitutive Methanosarcina acetivorans promoter was used to express MekB, a broad-specificity esterase from Pseudomonas veronii, in M. acetivorans. The engineered strain had 80-fold greater esterase activity than wild-type M. acetivorans. Methyl acetate and methyl propionate esters served as the sole carbon and energy sources, resulting in robust growth and methane formation, with consumption of >97% of the substrates. Methanol was undetectable at the end of growth with methyl acetate, whereas acetate accumulated, a result consistent with methanol as the more favorable substrate. Acetate was consumed, and growth continued after a period of adaptation. Similar results were obtained with methyl propionate, except propionate was not metabolized. IMPORTANCE The fragile interactions of multispecies food chains converting complex biomass to methane are easily disrupted, a major impediment to efficient and reliable conversion of renewable biomass as an alternative to fossil fuels. The hybrid pathway, derived by combining catabolic pathways from a methanogen of the domain Archaea and a strictly aerobic species of the domain Bacteria, catalyzes the complete conversion of an industrial solvent that is also a naturally occurring compound to methane and carbon dioxide. The engineered pathway expands the exceptionally narrow range of substrates utilized by methanogens, exemplifying the simplification of food chains leading to the more-efficient conversion of complex biomass to methane.Daniel J. LessnerLexan LhuChristopher S. WahalJames G. FerryAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 1, Iss 5 (2010)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Daniel J. Lessner Lexan Lhu Christopher S. Wahal James G. Ferry An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>
description	ABSTRACT A plasmid-based expression system wherein mekB was fused to a constitutive Methanosarcina acetivorans promoter was used to express MekB, a broad-specificity esterase from Pseudomonas veronii, in M. acetivorans. The engineered strain had 80-fold greater esterase activity than wild-type M. acetivorans. Methyl acetate and methyl propionate esters served as the sole carbon and energy sources, resulting in robust growth and methane formation, with consumption of >97% of the substrates. Methanol was undetectable at the end of growth with methyl acetate, whereas acetate accumulated, a result consistent with methanol as the more favorable substrate. Acetate was consumed, and growth continued after a period of adaptation. Similar results were obtained with methyl propionate, except propionate was not metabolized. IMPORTANCE The fragile interactions of multispecies food chains converting complex biomass to methane are easily disrupted, a major impediment to efficient and reliable conversion of renewable biomass as an alternative to fossil fuels. The hybrid pathway, derived by combining catabolic pathways from a methanogen of the domain Archaea and a strictly aerobic species of the domain Bacteria, catalyzes the complete conversion of an industrial solvent that is also a naturally occurring compound to methane and carbon dioxide. The engineered pathway expands the exceptionally narrow range of substrates utilized by methanogens, exemplifying the simplification of food chains leading to the more-efficient conversion of complex biomass to methane.
format	article
author	Daniel J. Lessner Lexan Lhu Christopher S. Wahal James G. Ferry
author_facet	Daniel J. Lessner Lexan Lhu Christopher S. Wahal James G. Ferry
author_sort	Daniel J. Lessner
title	An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>
title_short	An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>
title_full	An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>
title_fullStr	An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>
title_full_unstemmed	An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>
title_sort	engineered methanogenic pathway derived from the domains <italic toggle="yes">bacteria</italic> and <italic toggle="yes">archaea</italic>
publisher	American Society for Microbiology
publishDate	2010
url	https://doaj.org/article/ee2e5de390c84b4088971e70a94e55d7
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An Engineered Methanogenic Pathway Derived from the Domains <italic toggle="yes">Bacteria</italic> and <italic toggle="yes">Archaea</italic>

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