Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community

Abstract The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for ass...

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Autores principales:	Linda Eriksson, Pernilla Lif Holgerson, Ingegerd Johansson
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee3059ae40dd4236be95a17778be5979
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spelling	oai:doaj.org-article:ee3059ae40dd4236be95a17778be59792021-12-02T11:41:19ZSaliva and tooth biofilm bacterial microbiota in adolescents in a low caries community10.1038/s41598-017-06221-z2045-2322https://doaj.org/article/ee3059ae40dd4236be95a17778be59792017-07-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-06221-zhttps://doaj.org/toc/2045-2322Abstract The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for association with caries prevalence and incidence. Saliva and tooth biofilm from 17-year-olds and mock bacteria communities were analysed using 16S rDNA Illumina MiSeq (v3-v4) and PacBio SMRT (v1-v8) sequencing including validity and reliability estimates. Caries was scored at 17 and 19 years of age. Both sequencing platforms revealed that Firmicutes dominated in the saliva, whereas Firmicutes and Actinobacteria abundances were similar in tooth biofilm. Saliva microbiota discriminated caries-affected from caries-free adolescents, with enumeration of Scardovia wiggsiae, Streptococcus mutans, Bifidobacterium longum, Leptotrichia sp. HOT498, and Selenomonas spp. in caries-affected participants. Adolescents with B. longum in saliva had significantly higher 2-year caries increment. PacBio SMRT revealed Corynebacterium matruchotii as the most prevalent species in tooth biofilm. In conclusion, both sequencing methods were reliable and valid for oral samples, and saliva microbiota was associated with cross-sectional caries prevalence, especially S. wiggsiae, S. mutans, and B. longum; the latter also with the 2-year caries incidence.Linda ErikssonPernilla Lif HolgersonIngegerd JohanssonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-12 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Linda Eriksson Pernilla Lif Holgerson Ingegerd Johansson Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
description	Abstract The oral cavity harbours a complex microbiome that is linked to dental diseases and serves as a route to other parts of the body. Here, the aims were to characterize the oral microbiota by deep sequencing in a low-caries population with regular dental care since childhood and search for association with caries prevalence and incidence. Saliva and tooth biofilm from 17-year-olds and mock bacteria communities were analysed using 16S rDNA Illumina MiSeq (v3-v4) and PacBio SMRT (v1-v8) sequencing including validity and reliability estimates. Caries was scored at 17 and 19 years of age. Both sequencing platforms revealed that Firmicutes dominated in the saliva, whereas Firmicutes and Actinobacteria abundances were similar in tooth biofilm. Saliva microbiota discriminated caries-affected from caries-free adolescents, with enumeration of Scardovia wiggsiae, Streptococcus mutans, Bifidobacterium longum, Leptotrichia sp. HOT498, and Selenomonas spp. in caries-affected participants. Adolescents with B. longum in saliva had significantly higher 2-year caries increment. PacBio SMRT revealed Corynebacterium matruchotii as the most prevalent species in tooth biofilm. In conclusion, both sequencing methods were reliable and valid for oral samples, and saliva microbiota was associated with cross-sectional caries prevalence, especially S. wiggsiae, S. mutans, and B. longum; the latter also with the 2-year caries incidence.
format	article
author	Linda Eriksson Pernilla Lif Holgerson Ingegerd Johansson
author_facet	Linda Eriksson Pernilla Lif Holgerson Ingegerd Johansson
author_sort	Linda Eriksson
title	Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_short	Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_full	Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_fullStr	Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_full_unstemmed	Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
title_sort	saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/ee3059ae40dd4236be95a17778be5979
work_keys_str_mv	AT lindaeriksson salivaandtoothbiofilmbacterialmicrobiotainadolescentsinalowcariescommunity AT pernillalifholgerson salivaandtoothbiofilmbacterialmicrobiotainadolescentsinalowcariescommunity AT ingegerdjohansson salivaandtoothbiofilmbacterialmicrobiotainadolescentsinalowcariescommunity
_version_	1718395435978063872

Saliva and tooth biofilm bacterial microbiota in adolescents in a low caries community

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