High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination

Abstract High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to ac...

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Autores principales:	Azeem Ahmad, Vishesh Dubey, Nikhil Jayakumar, Anowarul Habib, Ankit Butola, Mona Nystad, Ganesh Acharya, Purusotam Basnet, Dalip Singh Mehta, Balpreet Singh Ahluwalia
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee3c392fc3a545fc93bd9984698cb487
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spelling	oai:doaj.org-article:ee3c392fc3a545fc93bd9984698cb4872021-12-02T14:53:43ZHigh-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination10.1038/s41598-021-94915-w2045-2322https://doaj.org/article/ee3c392fc3a545fc93bd9984698cb4872021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-94915-whttps://doaj.org/toc/2045-2322Abstract High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field of view (FOV). In addition, such light sources have low photon degeneracy. On the contrary, high temporal coherence light sources like lasers are capable of exploiting the full FOV of the QPM systems at the expense of less spatial phase sensitivity. In the present work, we demonstrated that use of narrowband partially spatially coherent light source also called pseudo-thermal light source (PTLS) in QPM overcomes the limitations of conventional light sources. The performance of PTLS is compared with conventional light sources in terms of space bandwidth product, phase sensitivity and optical imaging quality. The capabilities of PTLS are demonstrated on both amplitude (USAF resolution chart) and phase (thin optical waveguide, height ~ 8 nm) objects. The spatial phase sensitivity of QPM using PTLS is measured to be equivalent to that for white light source and supports the FOV (18 times more) equivalent to that of laser light source. The high-speed capabilities of PTLS based QPM is demonstrated by imaging live sperm cells that is limited by the camera speed and large FOV is demonstrated by imaging histopathology human placenta tissue samples. Minimal invasive, high-throughput, spatially sensitive and single-shot QPM based on PTLS will enable wider penetration of QPM in life sciences and clinical applications.Azeem AhmadVishesh DubeyNikhil JayakumarAnowarul HabibAnkit ButolaMona NystadGanesh AcharyaPurusotam BasnetDalip Singh MehtaBalpreet Singh AhluwaliaNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-13 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Azeem Ahmad Vishesh Dubey Nikhil Jayakumar Anowarul Habib Ankit Butola Mona Nystad Ganesh Acharya Purusotam Basnet Dalip Singh Mehta Balpreet Singh Ahluwalia High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
description	Abstract High space-bandwidth product with high spatial phase sensitivity is indispensable for a single-shot quantitative phase microscopy (QPM) system. It opens avenue for widespread applications of QPM in the field of biomedical imaging. Temporally low coherence light sources are implemented to achieve high spatial phase sensitivity in QPM at the cost of either reduced temporal resolution or smaller field of view (FOV). In addition, such light sources have low photon degeneracy. On the contrary, high temporal coherence light sources like lasers are capable of exploiting the full FOV of the QPM systems at the expense of less spatial phase sensitivity. In the present work, we demonstrated that use of narrowband partially spatially coherent light source also called pseudo-thermal light source (PTLS) in QPM overcomes the limitations of conventional light sources. The performance of PTLS is compared with conventional light sources in terms of space bandwidth product, phase sensitivity and optical imaging quality. The capabilities of PTLS are demonstrated on both amplitude (USAF resolution chart) and phase (thin optical waveguide, height ~ 8 nm) objects. The spatial phase sensitivity of QPM using PTLS is measured to be equivalent to that for white light source and supports the FOV (18 times more) equivalent to that of laser light source. The high-speed capabilities of PTLS based QPM is demonstrated by imaging live sperm cells that is limited by the camera speed and large FOV is demonstrated by imaging histopathology human placenta tissue samples. Minimal invasive, high-throughput, spatially sensitive and single-shot QPM based on PTLS will enable wider penetration of QPM in life sciences and clinical applications.
format	article
author	Azeem Ahmad Vishesh Dubey Nikhil Jayakumar Anowarul Habib Ankit Butola Mona Nystad Ganesh Acharya Purusotam Basnet Dalip Singh Mehta Balpreet Singh Ahluwalia
author_facet	Azeem Ahmad Vishesh Dubey Nikhil Jayakumar Anowarul Habib Ankit Butola Mona Nystad Ganesh Acharya Purusotam Basnet Dalip Singh Mehta Balpreet Singh Ahluwalia
author_sort	Azeem Ahmad
title	High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
title_short	High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
title_full	High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
title_fullStr	High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
title_full_unstemmed	High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
title_sort	high-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ee3c392fc3a545fc93bd9984698cb487
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High-throughput spatial sensitive quantitative phase microscopy using low spatial and high temporal coherent illumination

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