Exploring rare cellular activity in more than one million cells by a transscale scope

Abstract In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge...

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Autores principales:	T. Ichimura, T. Kakizuka, K. Horikawa, K. Seiriki, A. Kasai, H. Hashimoto, K. Fujita, T. M. Watanabe, T. Nagai
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee3f413ac7694f97808d340fb3263067
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spelling	oai:doaj.org-article:ee3f413ac7694f97808d340fb32630672021-12-02T15:10:34ZExploring rare cellular activity in more than one million cells by a transscale scope10.1038/s41598-021-95930-72045-2322https://doaj.org/article/ee3f413ac7694f97808d340fb32630672021-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-95930-7https://doaj.org/toc/2045-2322Abstract In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.T. IchimuraT. KakizukaK. HorikawaK. SeirikiA. KasaiH. HashimotoK. FujitaT. M. WatanabeT. NagaiNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-16 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q T. Ichimura T. Kakizuka K. Horikawa K. Seiriki A. Kasai H. Hashimoto K. Fujita T. M. Watanabe T. Nagai Exploring rare cellular activity in more than one million cells by a transscale scope
description	Abstract In many phenomena of biological systems, not a majority, but a minority of cells act on the entire multicellular system causing drastic changes in the system properties. To understand the mechanisms underlying such phenomena, it is essential to observe the spatiotemporal dynamics of a huge population of cells at sub-cellular resolution, which is difficult with conventional tools such as microscopy and flow cytometry. Here, we describe an imaging system named AMATERAS that enables optical imaging with an over-one-centimeter field-of-view and a-few-micrometer spatial resolution. This trans-scale-scope has a simple configuration, composed of a low-power lens for machine vision and a hundred-megapixel image sensor. We demonstrated its high cell-throughput, capable of simultaneously observing more than one million cells. We applied it to dynamic imaging of calcium ions in HeLa cells and cyclic-adenosine-monophosphate in Dictyostelium discoideum, and successfully detected less than 0.01% of rare cells and observed multicellular events induced by these cells.
format	article
author	T. Ichimura T. Kakizuka K. Horikawa K. Seiriki A. Kasai H. Hashimoto K. Fujita T. M. Watanabe T. Nagai
author_facet	T. Ichimura T. Kakizuka K. Horikawa K. Seiriki A. Kasai H. Hashimoto K. Fujita T. M. Watanabe T. Nagai
author_sort	T. Ichimura
title	Exploring rare cellular activity in more than one million cells by a transscale scope
title_short	Exploring rare cellular activity in more than one million cells by a transscale scope
title_full	Exploring rare cellular activity in more than one million cells by a transscale scope
title_fullStr	Exploring rare cellular activity in more than one million cells by a transscale scope
title_full_unstemmed	Exploring rare cellular activity in more than one million cells by a transscale scope
title_sort	exploring rare cellular activity in more than one million cells by a transscale scope
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ee3f413ac7694f97808d340fb3263067
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Exploring rare cellular activity in more than one million cells by a transscale scope

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