Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy

Neoadjuvant chemotherapy (NAC) is a standard treatment strategy for patients with locally advanced breast cancer (LABC). However, there are no established predictors of chemosensitivity and survival in LABC patients who undergo NAC. Many studies have demonstrated that exosomes and cytokines are impo...

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Autores principales:	Hae Hyun Jung, Ji-Yeon Kim, Eun Yoon Cho, Jung Min Oh, Jeong Eon Lee, Seok Won Kim, Seok Jin Nam, Yeon Hee Park, Jin Seok Ahn, Young-Hyuck Im
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	exosomes extracellular vesicles breast cancer neoadjuvant chemotherapy prognostic biomarker nerve growth factor Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
Acceso en línea:	https://doaj.org/article/ee4c6e9839564abaa39cf6e756281830
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spelling	oai:doaj.org-article:ee4c6e9839564abaa39cf6e7562818302021-11-11T15:26:34ZElevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy10.3390/cancers132152602072-6694https://doaj.org/article/ee4c6e9839564abaa39cf6e7562818302021-10-01T00:00:00Zhttps://www.mdpi.com/2072-6694/13/21/5260https://doaj.org/toc/2072-6694Neoadjuvant chemotherapy (NAC) is a standard treatment strategy for patients with locally advanced breast cancer (LABC). However, there are no established predictors of chemosensitivity and survival in LABC patients who undergo NAC. Many studies have demonstrated that exosomes and cytokines are important players in intercellular communication between tumors and their environments, and are involved in chemotherapy resistance. Recently, it was reported that cytokines can be packaged into exosomes, but whether exosomal cytokines serve as biomarkers in breast cancer patients is still unclear. In this study, we examined the roles of cytokines in both serum and exosomes as prognostic biomarkers for long-term outcomes in patients with breast cancer who undergo NAC. We isolated exosomes from the blood of 129 patients with early breast cancer who were receiving neoadjuvant chemotherapy between 2008 and 2011 at Samsung Medical Center. The levels of cytokines and growth factors in serum and exosomes were measured with ProcartaPlex immune-related panels. We investigated correlations between clinic-pathologic variables and patient survival, and Cox proportional hazards regression analysis was performed for prognostic evaluation. We detected significant differences in expression patterns between serum cytokines and exosomal cytokines. In both serum and exosomes, many cytokines were positively correlated with age. In univariate analysis, patients with high serum IP-10, serum MMP-1, and exosomal NGF had shorter overall survival. Exosomal NGF showed significantly poorer overall survival in multivariate analysis. These findings suggest that exosomal NGF is useful for identifying patients with poor survival outcomes.Hae Hyun JungJi-Yeon KimEun Yoon ChoJung Min OhJeong Eon LeeSeok Won KimSeok Jin NamYeon Hee ParkJin Seok AhnYoung-Hyuck ImMDPI AGarticleexosomesextracellular vesiclesbreast cancerneoadjuvant chemotherapyprognostic biomarkernerve growth factorNeoplasms. Tumors. Oncology. Including cancer and carcinogensRC254-282ENCancers, Vol 13, Iss 5260, p 5260 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	exosomes extracellular vesicles breast cancer neoadjuvant chemotherapy prognostic biomarker nerve growth factor Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282
spellingShingle	exosomes extracellular vesicles breast cancer neoadjuvant chemotherapy prognostic biomarker nerve growth factor Neoplasms. Tumors. Oncology. Including cancer and carcinogens RC254-282 Hae Hyun Jung Ji-Yeon Kim Eun Yoon Cho Jung Min Oh Jeong Eon Lee Seok Won Kim Seok Jin Nam Yeon Hee Park Jin Seok Ahn Young-Hyuck Im Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy
description	Neoadjuvant chemotherapy (NAC) is a standard treatment strategy for patients with locally advanced breast cancer (LABC). However, there are no established predictors of chemosensitivity and survival in LABC patients who undergo NAC. Many studies have demonstrated that exosomes and cytokines are important players in intercellular communication between tumors and their environments, and are involved in chemotherapy resistance. Recently, it was reported that cytokines can be packaged into exosomes, but whether exosomal cytokines serve as biomarkers in breast cancer patients is still unclear. In this study, we examined the roles of cytokines in both serum and exosomes as prognostic biomarkers for long-term outcomes in patients with breast cancer who undergo NAC. We isolated exosomes from the blood of 129 patients with early breast cancer who were receiving neoadjuvant chemotherapy between 2008 and 2011 at Samsung Medical Center. The levels of cytokines and growth factors in serum and exosomes were measured with ProcartaPlex immune-related panels. We investigated correlations between clinic-pathologic variables and patient survival, and Cox proportional hazards regression analysis was performed for prognostic evaluation. We detected significant differences in expression patterns between serum cytokines and exosomal cytokines. In both serum and exosomes, many cytokines were positively correlated with age. In univariate analysis, patients with high serum IP-10, serum MMP-1, and exosomal NGF had shorter overall survival. Exosomal NGF showed significantly poorer overall survival in multivariate analysis. These findings suggest that exosomal NGF is useful for identifying patients with poor survival outcomes.
format	article
author	Hae Hyun Jung Ji-Yeon Kim Eun Yoon Cho Jung Min Oh Jeong Eon Lee Seok Won Kim Seok Jin Nam Yeon Hee Park Jin Seok Ahn Young-Hyuck Im
author_facet	Hae Hyun Jung Ji-Yeon Kim Eun Yoon Cho Jung Min Oh Jeong Eon Lee Seok Won Kim Seok Jin Nam Yeon Hee Park Jin Seok Ahn Young-Hyuck Im
author_sort	Hae Hyun Jung
title	Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy
title_short	Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy
title_full	Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy
title_fullStr	Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy
title_full_unstemmed	Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy
title_sort	elevated level of nerve growth factor (ngf) in serum-derived exosomes predicts poor survival in patients with breast cancer undergoing neoadjuvant chemotherapy
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ee4c6e9839564abaa39cf6e756281830
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Elevated Level of Nerve Growth Factor (NGF) in Serum-Derived Exosomes Predicts Poor Survival in Patients with Breast Cancer Undergoing Neoadjuvant Chemotherapy

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