Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells

We have reported that nicotine has a neurotrophic action on peripheral adrenergic nerves in vivo, which is mediated by α7 nicotinic acetylcholine receptors (nAChRs). To clarify the possible mechanisms, the present study further investigated the effect of nicotine on neurite outgrowth in tyrosine hyd...

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Autores principales:	Hiromu Kawasaki, Hayato Hino, Fusako Takayama, Yoshihisa Kitamura, Toshiaki Sendou, Shingo Takatori
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2022
Materias:	Neurite outgrowth Nicotine Nicotinic acetylcholine receptors Rat superior cervical ganglia cells Therapeutics. Pharmacology RM1-950
Acceso en línea:	https://doaj.org/article/ee50beb85e3244da87bb80b1736f2747
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spelling	oai:doaj.org-article:ee50beb85e3244da87bb80b1736f27472021-11-12T04:27:36ZRegulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells1347-861310.1016/j.jphs.2021.10.012https://doaj.org/article/ee50beb85e3244da87bb80b1736f27472022-01-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S1347861321001079https://doaj.org/toc/1347-8613We have reported that nicotine has a neurotrophic action on peripheral adrenergic nerves in vivo, which is mediated by α7 nicotinic acetylcholine receptors (nAChRs). To clarify the possible mechanisms, the present study further investigated the effect of nicotine on neurite outgrowth in tyrosine hydroxylase (TH)-positive superior cervical ganglia (SCG) cells isolated from neonatal rats in vitro. Nicotine at low concentrations (0.01–0.3 mM) increased the number of neurite outgrowths in TH-immunopositive SCG cells, while high concentrations of nicotine (1–10 mM) gradually reduced it, and only 10 mM nicotine was markedly inhibited compared to the control. A 100 μM of nicotine-induced increase in neurite numbers depended on the exposure time and was inhibited by treatment with the nAChR antagonist hexamethonium (Hex) and α7 nAChR antagonist α-bungarotoxin (α-Bgtx). The nicotine (10 mM)-induced a significant decrease in neurite outgrowth in SCG, which was perfectly canceled by Hex to the control level but not by α-Bgtx. These results suggest that nicotine has a regulatory neurotrophic action mediated by both α7 nAChR and other subtypes in TH-positive SCG cells of rats.Hiromu KawasakiHayato HinoFusako TakayamaYoshihisa KitamuraToshiaki SendouShingo TakatoriElsevierarticleNeurite outgrowthNicotineNicotinic acetylcholine receptorsRat superior cervical ganglia cellsTherapeutics. PharmacologyRM1-950ENJournal of Pharmacological Sciences, Vol 148, Iss 1, Pp 103-107 (2022)
institution	DOAJ
collection	DOAJ
language	EN
topic	Neurite outgrowth Nicotine Nicotinic acetylcholine receptors Rat superior cervical ganglia cells Therapeutics. Pharmacology RM1-950
spellingShingle	Neurite outgrowth Nicotine Nicotinic acetylcholine receptors Rat superior cervical ganglia cells Therapeutics. Pharmacology RM1-950 Hiromu Kawasaki Hayato Hino Fusako Takayama Yoshihisa Kitamura Toshiaki Sendou Shingo Takatori Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
description	We have reported that nicotine has a neurotrophic action on peripheral adrenergic nerves in vivo, which is mediated by α7 nicotinic acetylcholine receptors (nAChRs). To clarify the possible mechanisms, the present study further investigated the effect of nicotine on neurite outgrowth in tyrosine hydroxylase (TH)-positive superior cervical ganglia (SCG) cells isolated from neonatal rats in vitro. Nicotine at low concentrations (0.01–0.3 mM) increased the number of neurite outgrowths in TH-immunopositive SCG cells, while high concentrations of nicotine (1–10 mM) gradually reduced it, and only 10 mM nicotine was markedly inhibited compared to the control. A 100 μM of nicotine-induced increase in neurite numbers depended on the exposure time and was inhibited by treatment with the nAChR antagonist hexamethonium (Hex) and α7 nAChR antagonist α-bungarotoxin (α-Bgtx). The nicotine (10 mM)-induced a significant decrease in neurite outgrowth in SCG, which was perfectly canceled by Hex to the control level but not by α-Bgtx. These results suggest that nicotine has a regulatory neurotrophic action mediated by both α7 nAChR and other subtypes in TH-positive SCG cells of rats.
format	article
author	Hiromu Kawasaki Hayato Hino Fusako Takayama Yoshihisa Kitamura Toshiaki Sendou Shingo Takatori
author_facet	Hiromu Kawasaki Hayato Hino Fusako Takayama Yoshihisa Kitamura Toshiaki Sendou Shingo Takatori
author_sort	Hiromu Kawasaki
title	Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
title_short	Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
title_full	Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
title_fullStr	Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
title_full_unstemmed	Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
title_sort	regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells
publisher	Elsevier
publishDate	2022
url	https://doaj.org/article/ee50beb85e3244da87bb80b1736f2747
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Regulatory effects of nicotine on neurite outgrowth in rat superior cervical ganglia cells

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