SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.

Nuclear scaffold attachment factor A (SAFA) is a novel RNA sensor involved in sensing viral RNA in the nucleus and mediating antiviral immunity. Severe fever with thrombocytopenia syndrome virus (SFTSV) is a bunyavirus that causes SFTS with a high fatality rate of up to 30%. It remains elusive wheth...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Bin-Yan Liu, Xue-Jie Yu, Chuan-Min Zhou
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2021
Materias:	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ee5435f6203f46b480612935394385ee
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee5435f6203f46b480612935394385ee
record_format	dspace
spelling	oai:doaj.org-article:ee5435f6203f46b480612935394385ee2021-12-02T19:59:52ZSAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.1553-73661553-737410.1371/journal.ppat.1010070https://doaj.org/article/ee5435f6203f46b480612935394385ee2021-11-01T00:00:00Zhttps://doi.org/10.1371/journal.ppat.1010070https://doaj.org/toc/1553-7366https://doaj.org/toc/1553-7374Nuclear scaffold attachment factor A (SAFA) is a novel RNA sensor involved in sensing viral RNA in the nucleus and mediating antiviral immunity. Severe fever with thrombocytopenia syndrome virus (SFTSV) is a bunyavirus that causes SFTS with a high fatality rate of up to 30%. It remains elusive whether and how cytoplasmic SFTSV can be sensed by the RNA sensor SAFA. Here, we demonstrated that SAFA was able to detect SFTSV infection and mediate antiviral interferon and inflammatory responses. Transcription and expression levels of SAFA were strikingly upregulated under SFTSV infection. SAFA was retained in the cytoplasm by interaction with SFTSV nucleocapsid protein (NP). Importantly, SFTSV genomic RNA was recognized by cytoplasmic SAFA, which recruited and promoted activation of the STING-TBK1 signaling axis against SFTSV infection. Of note, the nuclear localization signal (NLS) domain of SAFA was important for interaction with SFTSV NP and recognition of SFTSV RNA in the cytoplasm. In conclusion, our study reveals a novel antiviral mechanism in which SAFA functions as a novel cytoplasmic RNA sensor that directly recognizes RNA virus SFTSV and mediates an antiviral response.Bin-Yan LiuXue-Jie YuChuan-Min ZhouPublic Library of Science (PLoS)articleImmunologic diseases. AllergyRC581-607Biology (General)QH301-705.5ENPLoS Pathogens, Vol 17, Iss 11, p e1010070 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5
spellingShingle	Immunologic diseases. Allergy RC581-607 Biology (General) QH301-705.5 Bin-Yan Liu Xue-Jie Yu Chuan-Min Zhou SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.
description	Nuclear scaffold attachment factor A (SAFA) is a novel RNA sensor involved in sensing viral RNA in the nucleus and mediating antiviral immunity. Severe fever with thrombocytopenia syndrome virus (SFTSV) is a bunyavirus that causes SFTS with a high fatality rate of up to 30%. It remains elusive whether and how cytoplasmic SFTSV can be sensed by the RNA sensor SAFA. Here, we demonstrated that SAFA was able to detect SFTSV infection and mediate antiviral interferon and inflammatory responses. Transcription and expression levels of SAFA were strikingly upregulated under SFTSV infection. SAFA was retained in the cytoplasm by interaction with SFTSV nucleocapsid protein (NP). Importantly, SFTSV genomic RNA was recognized by cytoplasmic SAFA, which recruited and promoted activation of the STING-TBK1 signaling axis against SFTSV infection. Of note, the nuclear localization signal (NLS) domain of SAFA was important for interaction with SFTSV NP and recognition of SFTSV RNA in the cytoplasm. In conclusion, our study reveals a novel antiviral mechanism in which SAFA functions as a novel cytoplasmic RNA sensor that directly recognizes RNA virus SFTSV and mediates an antiviral response.
format	article
author	Bin-Yan Liu Xue-Jie Yu Chuan-Min Zhou
author_facet	Bin-Yan Liu Xue-Jie Yu Chuan-Min Zhou
author_sort	Bin-Yan Liu
title	SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.
title_short	SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.
title_full	SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.
title_fullStr	SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.
title_full_unstemmed	SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.
title_sort	safa initiates innate immunity against cytoplasmic rna virus sftsv infection.
publisher	Public Library of Science (PLoS)
publishDate	2021
url	https://doaj.org/article/ee5435f6203f46b480612935394385ee
work_keys_str_mv	AT binyanliu safainitiatesinnateimmunityagainstcytoplasmicrnavirussftsvinfection AT xuejieyu safainitiatesinnateimmunityagainstcytoplasmicrnavirussftsvinfection AT chuanminzhou safainitiatesinnateimmunityagainstcytoplasmicrnavirussftsvinfection
_version_	1718375755380948992

SAFA initiates innate immunity against cytoplasmic RNA virus SFTSV infection.

Ejemplares similares