Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia

Abstract 16S rRNA sequencing of human fecal samples has been tremendously successful in identifying microbiome changes associated with both aging and disease. A number of studies have described microbial alterations corresponding to physical frailty and nursing home residence among aging individuals...

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Autores principales:	Lin Kang, Pengtao Li, Danyang Wang, Taihao Wang, Dong Hao, Xuan Qu
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee5ab244d47b49f2abf3fb935bef0b2b
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spelling	oai:doaj.org-article:ee5ab244d47b49f2abf3fb935bef0b2b2021-12-02T13:30:11ZAlterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia10.1038/s41598-021-84031-02045-2322https://doaj.org/article/ee5ab244d47b49f2abf3fb935bef0b2b2021-02-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-84031-0https://doaj.org/toc/2045-2322Abstract 16S rRNA sequencing of human fecal samples has been tremendously successful in identifying microbiome changes associated with both aging and disease. A number of studies have described microbial alterations corresponding to physical frailty and nursing home residence among aging individuals. A gut-muscle axis through which the microbiome influences skeletal muscle growth/function has been hypothesized. However, the microbiome has yet to be examined in sarcopenia. Here, we collected fecal samples of 60 healthy controls (CON) and 27 sarcopenic (Case)/possibly sarcopenic (preCase) individuals and analyzed the intestinal microbiota using 16S rRNA sequencing. We observed an overall reduction in microbial diversity in Case and preCase samples. The genera Lachnospira, Fusicantenibacter, Roseburia, Eubacterium, and Lachnoclostridium—known butyrate producers—were significantly less abundant in Case and preCase subjects while Lactobacillus was more abundant. Functional pathways underrepresented in Case subjects included numerous transporters and phenylalanine, tyrosine, and tryptophan biosynthesis suggesting that protein processing and nutrient transport may be impaired. In contrast, lipopolysaccharide biosynthesis was overrepresented in Case and PreCase subjects suggesting that sarcopenia is associated with a pro-inflammatory metagenome. These analyses demonstrate structural and functional alterations in the intestinal microbiota that may contribute to loss of skeletal muscle mass and function in sarcopenia.Lin KangPengtao LiDanyang WangTaihao WangDong HaoXuan QuNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Lin Kang Pengtao Li Danyang Wang Taihao Wang Dong Hao Xuan Qu Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
description	Abstract 16S rRNA sequencing of human fecal samples has been tremendously successful in identifying microbiome changes associated with both aging and disease. A number of studies have described microbial alterations corresponding to physical frailty and nursing home residence among aging individuals. A gut-muscle axis through which the microbiome influences skeletal muscle growth/function has been hypothesized. However, the microbiome has yet to be examined in sarcopenia. Here, we collected fecal samples of 60 healthy controls (CON) and 27 sarcopenic (Case)/possibly sarcopenic (preCase) individuals and analyzed the intestinal microbiota using 16S rRNA sequencing. We observed an overall reduction in microbial diversity in Case and preCase samples. The genera Lachnospira, Fusicantenibacter, Roseburia, Eubacterium, and Lachnoclostridium—known butyrate producers—were significantly less abundant in Case and preCase subjects while Lactobacillus was more abundant. Functional pathways underrepresented in Case subjects included numerous transporters and phenylalanine, tyrosine, and tryptophan biosynthesis suggesting that protein processing and nutrient transport may be impaired. In contrast, lipopolysaccharide biosynthesis was overrepresented in Case and PreCase subjects suggesting that sarcopenia is associated with a pro-inflammatory metagenome. These analyses demonstrate structural and functional alterations in the intestinal microbiota that may contribute to loss of skeletal muscle mass and function in sarcopenia.
format	article
author	Lin Kang Pengtao Li Danyang Wang Taihao Wang Dong Hao Xuan Qu
author_facet	Lin Kang Pengtao Li Danyang Wang Taihao Wang Dong Hao Xuan Qu
author_sort	Lin Kang
title	Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
title_short	Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
title_full	Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
title_fullStr	Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
title_full_unstemmed	Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
title_sort	alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ee5ab244d47b49f2abf3fb935bef0b2b
work_keys_str_mv	AT linkang alterationsinintestinalmicrobiotadiversitycompositionandfunctioninpatientswithsarcopenia AT pengtaoli alterationsinintestinalmicrobiotadiversitycompositionandfunctioninpatientswithsarcopenia AT danyangwang alterationsinintestinalmicrobiotadiversitycompositionandfunctioninpatientswithsarcopenia AT taihaowang alterationsinintestinalmicrobiotadiversitycompositionandfunctioninpatientswithsarcopenia AT donghao alterationsinintestinalmicrobiotadiversitycompositionandfunctioninpatientswithsarcopenia AT xuanqu alterationsinintestinalmicrobiotadiversitycompositionandfunctioninpatientswithsarcopenia
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Alterations in intestinal microbiota diversity, composition, and function in patients with sarcopenia

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