Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa

R Salomoni,1,2 P Léo,2 AF Montemor,2 BG Rinaldi,2 MFA Rodrigues2 1Biotechnology Interunits Pos Graduation Program, University of Sao Paulo – USP, Butantan Institute, Institute for Technological Research – IPT, Sao Paulo, SP, Brazil; 2Industrial Biotechnology Laboratory...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Salomoni R, Léo P, Montemor AF, Rinaldi BG, Rodrigues MFA
Formato:	article
Lenguaje:	EN
Publicado:	Dove Medical Press 2017
Materias:	Antimicrobial activity Bacterial resistance Pseudomonas aeruginosa Silver Nanoparticles. Medical technology R855-855.5 Chemical technology TP1-1185
Acceso en línea:	https://doaj.org/article/ee5d694bb8ec4bdd8346acc852283a17
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee5d694bb8ec4bdd8346acc852283a17
record_format	dspace
spelling	oai:doaj.org-article:ee5d694bb8ec4bdd8346acc852283a172021-12-02T06:55:35ZAntibacterial effect of silver nanoparticles in Pseudomonas aeruginosa1177-8903https://doaj.org/article/ee5d694bb8ec4bdd8346acc852283a172017-06-01T00:00:00Zhttps://www.dovepress.com/antibacterial-effect-of-silver-nanoparticles-in-pseudomonas-aeruginosa-peer-reviewed-article-NSAhttps://doaj.org/toc/1177-8903R Salomoni,1,2 P Léo,2 AF Montemor,2 BG Rinaldi,2 MFA Rodrigues2 1Biotechnology Interunits Pos Graduation Program, University of Sao Paulo – USP, Butantan Institute, Institute for Technological Research – IPT, Sao Paulo, SP, Brazil; 2Industrial Biotechnology Laboratory, Bionanomanufacture Nucleus, Institute for Technological Research – IPT, São Paulo, SP, Brazil Abstract: Pseudomonas aeruginosa has great intrinsic antimicrobial resistance limiting the number of effective antibiotics. Thus, other antimicrobial agents such as silver nanoparticles (AgNPs) are considered potential agents to help manage and prevent infections. AgNPs can be used in several applications against bacteria resistant to common antibiotics or even multiresistant bacteria such as P. aeruginosa. This study assessed the antimicrobial activity of commercial 10 nm AgNPs on two hospital strains of P. aeruginosa resistant to a large number of antibiotics and a reference strain from a culture collection. All strains were susceptible to 5 µg/mL nanoparticles solution. Reference strains INCQS 0230 and P.a.1 were sensitive to AgNPs at concentrations of 1.25 and 0.156 µg/mL, respectively; however, this was not observed for hospital strain P.a.2, which was more resistant to all antibiotics and AgNPs tested. Cytotoxicity evaluation indicated that AgNPs, up to a concentration of 2.5 µg/mL, are very safe for all cell lines tested. At 5.0 µg/mL, AgNPs had a discrete cytotoxic effect on tumor cells HeLa and HepG2. Results showed the potential of using AgNPs as an alternative to conventional antimicrobial agents that are currently used, and a perspective for application of nanosilver with antibiotics to enhance antimicrobial activity. Keywords: antimicrobial activity, bacterial resistance, Pseudomonas aeruginosa, silver nanoparticles, AgNPsSalomoni RLéo PMontemor AFRinaldi BGRodrigues MFADove Medical PressarticleAntimicrobial activityBacterial resistancePseudomonas aeruginosaSilver Nanoparticles.Medical technologyR855-855.5Chemical technologyTP1-1185ENNanotechnology, Science and Applications, Vol Volume 10, Pp 115-121 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Antimicrobial activity Bacterial resistance Pseudomonas aeruginosa Silver Nanoparticles. Medical technology R855-855.5 Chemical technology TP1-1185
spellingShingle	Antimicrobial activity Bacterial resistance Pseudomonas aeruginosa Silver Nanoparticles. Medical technology R855-855.5 Chemical technology TP1-1185 Salomoni R Léo P Montemor AF Rinaldi BG Rodrigues MFA Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa
description	R Salomoni,1,2 P Léo,2 AF Montemor,2 BG Rinaldi,2 MFA Rodrigues2 1Biotechnology Interunits Pos Graduation Program, University of Sao Paulo – USP, Butantan Institute, Institute for Technological Research – IPT, Sao Paulo, SP, Brazil; 2Industrial Biotechnology Laboratory, Bionanomanufacture Nucleus, Institute for Technological Research – IPT, São Paulo, SP, Brazil Abstract: Pseudomonas aeruginosa has great intrinsic antimicrobial resistance limiting the number of effective antibiotics. Thus, other antimicrobial agents such as silver nanoparticles (AgNPs) are considered potential agents to help manage and prevent infections. AgNPs can be used in several applications against bacteria resistant to common antibiotics or even multiresistant bacteria such as P. aeruginosa. This study assessed the antimicrobial activity of commercial 10 nm AgNPs on two hospital strains of P. aeruginosa resistant to a large number of antibiotics and a reference strain from a culture collection. All strains were susceptible to 5 µg/mL nanoparticles solution. Reference strains INCQS 0230 and P.a.1 were sensitive to AgNPs at concentrations of 1.25 and 0.156 µg/mL, respectively; however, this was not observed for hospital strain P.a.2, which was more resistant to all antibiotics and AgNPs tested. Cytotoxicity evaluation indicated that AgNPs, up to a concentration of 2.5 µg/mL, are very safe for all cell lines tested. At 5.0 µg/mL, AgNPs had a discrete cytotoxic effect on tumor cells HeLa and HepG2. Results showed the potential of using AgNPs as an alternative to conventional antimicrobial agents that are currently used, and a perspective for application of nanosilver with antibiotics to enhance antimicrobial activity. Keywords: antimicrobial activity, bacterial resistance, Pseudomonas aeruginosa, silver nanoparticles, AgNPs
format	article
author	Salomoni R Léo P Montemor AF Rinaldi BG Rodrigues MFA
author_facet	Salomoni R Léo P Montemor AF Rinaldi BG Rodrigues MFA
author_sort	Salomoni R
title	Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa
title_short	Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa
title_full	Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa
title_fullStr	Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa
title_full_unstemmed	Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa
title_sort	antibacterial effect of silver nanoparticles in pseudomonas aeruginosa
publisher	Dove Medical Press
publishDate	2017
url	https://doaj.org/article/ee5d694bb8ec4bdd8346acc852283a17
work_keys_str_mv	AT salomonir antibacterialeffectofsilvernanoparticlesinpseudomonasaeruginosa AT leop antibacterialeffectofsilvernanoparticlesinpseudomonasaeruginosa AT montemoraf antibacterialeffectofsilvernanoparticlesinpseudomonasaeruginosa AT rinaldibg antibacterialeffectofsilvernanoparticlesinpseudomonasaeruginosa AT rodriguesmfa antibacterialeffectofsilvernanoparticlesinpseudomonasaeruginosa
_version_	1718399727187263488

Antibacterial effect of silver nanoparticles in Pseudomonas aeruginosa

Ejemplares similares