Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.

<h4>Background</h4>In the European Union (EU), the use of diniconazole-M is no longer authorized. However, residues of diniconazole-M occur in various plant commodities.<h4>Methodology/principal findings</h4>A selective and simple analytical method for the trace level determi...

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Autores principales:	Zhenjiang Liu, Yahui Jin, Minghua Wang
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee6f8798daab4f08aeb657529db90ba5
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spelling	oai:doaj.org-article:ee6f8798daab4f08aeb657529db90ba52021-11-18T08:12:50ZDetermination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.1932-620310.1371/journal.pone.0046929https://doaj.org/article/ee6f8798daab4f08aeb657529db90ba52012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23056532/?tool=EBIhttps://doaj.org/toc/1932-6203<h4>Background</h4>In the European Union (EU), the use of diniconazole-M is no longer authorized. However, residues of diniconazole-M occur in various plant commodities.<h4>Methodology/principal findings</h4>A selective and simple analytical method for the trace level determination of diniconazole in soil, fruit, vegetables and water samples was developed based on immunoaffinity extraction followed by Enzyme-linked immunosorbent assay (ELISA) and the high-performance liquid chromatography (HPLC) analysis. The ELISA was based on monoclonal antibodies highly specific to diniconazole and was a fast, cost-effective, and selective screening method for the detection of diniconazole. The results of the ELISA correlated well with gas chromatography (GC) results, with the correlation coefficient of 0.9879 (n = 19). A simple gel permeation chromato- graphy clean-up method was developed to purify extracts from matrices containing high amounts of fat and natural pigments, without the need for a large dilution of the sample. The immunoaffinity column (IAC) capacity was 0.180 mg g(-1). The columns could be re-used approximately 20 times with no significant alteration in capacity. The recoveries from complex samples were in the range of 89.2% to 96.1% with a relative standard deviation (RSD) of 0.770%-6.11% by ELISA. The results were in good agreement with those obtained by HPLC method.<h4>Conclusion/significance</h4>The IAC extraction procedure coupled with HPLC and ELISA analysis could be also used as alternative effective analytical methods for the determination of diniconazole concentrations in complex samples.Zhenjiang LiuYahui JinMinghua WangPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 10, p e46929 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Zhenjiang Liu Yahui Jin Minghua Wang Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.
description	<h4>Background</h4>In the European Union (EU), the use of diniconazole-M is no longer authorized. However, residues of diniconazole-M occur in various plant commodities.<h4>Methodology/principal findings</h4>A selective and simple analytical method for the trace level determination of diniconazole in soil, fruit, vegetables and water samples was developed based on immunoaffinity extraction followed by Enzyme-linked immunosorbent assay (ELISA) and the high-performance liquid chromatography (HPLC) analysis. The ELISA was based on monoclonal antibodies highly specific to diniconazole and was a fast, cost-effective, and selective screening method for the detection of diniconazole. The results of the ELISA correlated well with gas chromatography (GC) results, with the correlation coefficient of 0.9879 (n = 19). A simple gel permeation chromato- graphy clean-up method was developed to purify extracts from matrices containing high amounts of fat and natural pigments, without the need for a large dilution of the sample. The immunoaffinity column (IAC) capacity was 0.180 mg g(-1). The columns could be re-used approximately 20 times with no significant alteration in capacity. The recoveries from complex samples were in the range of 89.2% to 96.1% with a relative standard deviation (RSD) of 0.770%-6.11% by ELISA. The results were in good agreement with those obtained by HPLC method.<h4>Conclusion/significance</h4>The IAC extraction procedure coupled with HPLC and ELISA analysis could be also used as alternative effective analytical methods for the determination of diniconazole concentrations in complex samples.
format	article
author	Zhenjiang Liu Yahui Jin Minghua Wang
author_facet	Zhenjiang Liu Yahui Jin Minghua Wang
author_sort	Zhenjiang Liu
title	Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.
title_short	Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.
title_full	Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.
title_fullStr	Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.
title_full_unstemmed	Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.
title_sort	determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with hplc and elisa.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ee6f8798daab4f08aeb657529db90ba5
work_keys_str_mv	AT zhenjiangliu determinationofdiniconazoleinagriculturalsamplesbysolgelimmunoaffinityextractionprocedurecoupledwithhplcandelisa AT yahuijin determinationofdiniconazoleinagriculturalsamplesbysolgelimmunoaffinityextractionprocedurecoupledwithhplcandelisa AT minghuawang determinationofdiniconazoleinagriculturalsamplesbysolgelimmunoaffinityextractionprocedurecoupledwithhplcandelisa
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Determination of diniconazole in agricultural samples by sol-gel immunoaffinity extraction procedure coupled with HPLC and ELISA.

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