Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

Rhodobacter capsulatus NAD+ dependent formate dehydrogenase (RcFDH) is a molybdoenzyme that catalyses the reversible oxidation of formate to carbon dioxide, and is of interest for biotechnological applications. Here the authors present the cryo-EM structures of RcFDH as isolated from R. capsulatus a...

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Autores principales:	Christin Radon, Gerd Mittelstädt, Benjamin R. Duffus, Jörg Bürger, Tobias Hartmann, Thorsten Mielke, Christian Teutloff, Silke Leimkühler, Petra Wendler
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2020
Materias:	Science Q
Acceso en línea:	https://doaj.org/article/ee6fb5dae1e445018b0579d644893722
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spelling	oai:doaj.org-article:ee6fb5dae1e445018b0579d6448937222021-12-02T14:42:48ZCryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase10.1038/s41467-020-15614-02041-1723https://doaj.org/article/ee6fb5dae1e445018b0579d6448937222020-04-01T00:00:00Zhttps://doi.org/10.1038/s41467-020-15614-0https://doaj.org/toc/2041-1723Rhodobacter capsulatus NAD+ dependent formate dehydrogenase (RcFDH) is a molybdoenzyme that catalyses the reversible oxidation of formate to carbon dioxide, and is of interest for biotechnological applications. Here the authors present the cryo-EM structures of RcFDH as isolated from R. capsulatus and in the reduced state with bound NADH, and discuss the enzyme mechanism.Christin RadonGerd MittelstädtBenjamin R. DuffusJörg BürgerTobias HartmannThorsten MielkeChristian TeutloffSilke LeimkühlerPetra WendlerNature PortfolioarticleScienceQENNature Communications, Vol 11, Iss 1, Pp 1-9 (2020)
institution	DOAJ
collection	DOAJ
language	EN
topic	Science Q
spellingShingle	Science Q Christin Radon Gerd Mittelstädt Benjamin R. Duffus Jörg Bürger Tobias Hartmann Thorsten Mielke Christian Teutloff Silke Leimkühler Petra Wendler Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase
description	Rhodobacter capsulatus NAD+ dependent formate dehydrogenase (RcFDH) is a molybdoenzyme that catalyses the reversible oxidation of formate to carbon dioxide, and is of interest for biotechnological applications. Here the authors present the cryo-EM structures of RcFDH as isolated from R. capsulatus and in the reduced state with bound NADH, and discuss the enzyme mechanism.
format	article
author	Christin Radon Gerd Mittelstädt Benjamin R. Duffus Jörg Bürger Tobias Hartmann Thorsten Mielke Christian Teutloff Silke Leimkühler Petra Wendler
author_facet	Christin Radon Gerd Mittelstädt Benjamin R. Duffus Jörg Bürger Tobias Hartmann Thorsten Mielke Christian Teutloff Silke Leimkühler Petra Wendler
author_sort	Christin Radon
title	Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase
title_short	Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase
title_full	Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase
title_fullStr	Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase
title_full_unstemmed	Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase
title_sort	cryo-em structures reveal intricate fe-s cluster arrangement and charging in rhodobacter capsulatus formate dehydrogenase
publisher	Nature Portfolio
publishDate	2020
url	https://doaj.org/article/ee6fb5dae1e445018b0579d644893722
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Cryo-EM structures reveal intricate Fe-S cluster arrangement and charging in Rhodobacter capsulatus formate dehydrogenase

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