Aneugenic effects of epirubicin in somatic and germinal cells of male mice.

The ability of the antineoplastic agent epirubicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positiv...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Sabry Mohamed Attia, Sheikh Fayaz Ahmad, Radwa Mohamed Okash, Saleh Abdulrahman Bakheet
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee728bd0dcf14e3a85c1241d399e04a7
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee728bd0dcf14e3a85c1241d399e04a7
record_format	dspace
spelling	oai:doaj.org-article:ee728bd0dcf14e3a85c1241d399e04a72021-11-25T05:57:06ZAneugenic effects of epirubicin in somatic and germinal cells of male mice.1932-620310.1371/journal.pone.0109942https://doaj.org/article/ee728bd0dcf14e3a85c1241d399e04a72014-01-01T00:00:00Zhttps://doi.org/10.1371/journal.pone.0109942https://doaj.org/toc/1932-6203The ability of the antineoplastic agent epirubicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positive controls for clastogen and aneugen, respectively, and these compounds produced the expected responses. The fluorescence in situ hybridization assay with a centromeric DNA probe for erythrocyte micronuclei showed that epirubicin is not only clastogenic but also aneugenic in somatic cells in vivo. By using the BrdU-incorporation assay, it could be shown that the meiotic delay caused by epirubicin in germ cells was approximately 48 h. Disomic and diploid sperm were shown in epididymal sperm hybridized with DNA probes specific for chromosomes 8, X and Y after epirubicin treatment. The observation that XX- and YY-sperm significantly prevailed over XY-sperm indicates missegregation during the second meiotic division. The results also suggest that earlier prophase stages contribute less to epirubicin-induced aneuploidy. Both the clastogenic and aneugenic potential of epirubicin can give rise to the development of secondary tumors and abnormal reproductive outcomes in cured cancer patients and medical personnel exposed to epirubicin.Sabry Mohamed AttiaSheikh Fayaz AhmadRadwa Mohamed OkashSaleh Abdulrahman BakheetPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 10, p e109942 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Sabry Mohamed Attia Sheikh Fayaz Ahmad Radwa Mohamed Okash Saleh Abdulrahman Bakheet Aneugenic effects of epirubicin in somatic and germinal cells of male mice.
description	The ability of the antineoplastic agent epirubicin to induce aneuploidy and meiotic delay in the somatic and germinal cells of male mice was investigated by fluorescence in situ hybridization assay using labeled DNA probes and BrdU-incorporation assay. Mitomycin C and colchicine were used as positive controls for clastogen and aneugen, respectively, and these compounds produced the expected responses. The fluorescence in situ hybridization assay with a centromeric DNA probe for erythrocyte micronuclei showed that epirubicin is not only clastogenic but also aneugenic in somatic cells in vivo. By using the BrdU-incorporation assay, it could be shown that the meiotic delay caused by epirubicin in germ cells was approximately 48 h. Disomic and diploid sperm were shown in epididymal sperm hybridized with DNA probes specific for chromosomes 8, X and Y after epirubicin treatment. The observation that XX- and YY-sperm significantly prevailed over XY-sperm indicates missegregation during the second meiotic division. The results also suggest that earlier prophase stages contribute less to epirubicin-induced aneuploidy. Both the clastogenic and aneugenic potential of epirubicin can give rise to the development of secondary tumors and abnormal reproductive outcomes in cured cancer patients and medical personnel exposed to epirubicin.
format	article
author	Sabry Mohamed Attia Sheikh Fayaz Ahmad Radwa Mohamed Okash Saleh Abdulrahman Bakheet
author_facet	Sabry Mohamed Attia Sheikh Fayaz Ahmad Radwa Mohamed Okash Saleh Abdulrahman Bakheet
author_sort	Sabry Mohamed Attia
title	Aneugenic effects of epirubicin in somatic and germinal cells of male mice.
title_short	Aneugenic effects of epirubicin in somatic and germinal cells of male mice.
title_full	Aneugenic effects of epirubicin in somatic and germinal cells of male mice.
title_fullStr	Aneugenic effects of epirubicin in somatic and germinal cells of male mice.
title_full_unstemmed	Aneugenic effects of epirubicin in somatic and germinal cells of male mice.
title_sort	aneugenic effects of epirubicin in somatic and germinal cells of male mice.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ee728bd0dcf14e3a85c1241d399e04a7
work_keys_str_mv	AT sabrymohamedattia aneugeniceffectsofepirubicininsomaticandgerminalcellsofmalemice AT sheikhfayazahmad aneugeniceffectsofepirubicininsomaticandgerminalcellsofmalemice AT radwamohamedokash aneugeniceffectsofepirubicininsomaticandgerminalcellsofmalemice AT salehabdulrahmanbakheet aneugeniceffectsofepirubicininsomaticandgerminalcellsofmalemice
_version_	1718414376203976704

Aneugenic effects of epirubicin in somatic and germinal cells of male mice.

Ejemplares similares