Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.

Astrocytes are the most abundant cell type in the central nervous system (CNS) and have a multitude of functions that include maintenance of CNS homeostasis, trophic support of neurons, detoxification, and immune surveillance. It has only recently been appreciated that astrocyte dysfunction is a pri...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Nasir Malik, Xiantao Wang, Sonia Shah, Anastasia G Efthymiou, Bin Yan, Sabrina Heman-Ackah, Ming Zhan, Mahendra Rao
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2014
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee7c380eedef486580b14bf3ce02f19f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee7c380eedef486580b14bf3ce02f19f
record_format	dspace
spelling	oai:doaj.org-article:ee7c380eedef486580b14bf3ce02f19f2021-11-18T08:18:29ZComparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.1932-620310.1371/journal.pone.0096139https://doaj.org/article/ee7c380eedef486580b14bf3ce02f19f2014-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24848099/?tool=EBIhttps://doaj.org/toc/1932-6203Astrocytes are the most abundant cell type in the central nervous system (CNS) and have a multitude of functions that include maintenance of CNS homeostasis, trophic support of neurons, detoxification, and immune surveillance. It has only recently been appreciated that astrocyte dysfunction is a primary cause of many neurological disorders. Despite their importance in disease very little is known about global gene expression for human astrocytes. We have performed a microarray expression analysis of human fetal astrocytes to identify genes and signaling pathways that are important for astrocyte development and maintenance. Our analysis confirmed that the fetal astrocytes express high levels of the core astrocyte marker GFAP and the transcription factors from the NFI family which have been shown to play important roles in astrocyte development. A group of novel markers were identified that distinguish fetal astrocytes from pluripotent stem cell-derived neural stem cells (NSCs) and NSC-derived neurons. As in murine astrocytes, the Notch signaling pathway appears to be particularly important for cell fate decisions between the astrocyte and neuronal lineages in human astrocytes. These findings unveil the repertoire of genes expressed in human astrocytes and serve as a basis for further studies to better understand astrocyte biology, especially as it relates to disease.Nasir MalikXiantao WangSonia ShahAnastasia G EfthymiouBin YanSabrina Heman-AckahMing ZhanMahendra RaoPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 9, Iss 5, p e96139 (2014)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Nasir Malik Xiantao Wang Sonia Shah Anastasia G Efthymiou Bin Yan Sabrina Heman-Ackah Ming Zhan Mahendra Rao Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
description	Astrocytes are the most abundant cell type in the central nervous system (CNS) and have a multitude of functions that include maintenance of CNS homeostasis, trophic support of neurons, detoxification, and immune surveillance. It has only recently been appreciated that astrocyte dysfunction is a primary cause of many neurological disorders. Despite their importance in disease very little is known about global gene expression for human astrocytes. We have performed a microarray expression analysis of human fetal astrocytes to identify genes and signaling pathways that are important for astrocyte development and maintenance. Our analysis confirmed that the fetal astrocytes express high levels of the core astrocyte marker GFAP and the transcription factors from the NFI family which have been shown to play important roles in astrocyte development. A group of novel markers were identified that distinguish fetal astrocytes from pluripotent stem cell-derived neural stem cells (NSCs) and NSC-derived neurons. As in murine astrocytes, the Notch signaling pathway appears to be particularly important for cell fate decisions between the astrocyte and neuronal lineages in human astrocytes. These findings unveil the repertoire of genes expressed in human astrocytes and serve as a basis for further studies to better understand astrocyte biology, especially as it relates to disease.
format	article
author	Nasir Malik Xiantao Wang Sonia Shah Anastasia G Efthymiou Bin Yan Sabrina Heman-Ackah Ming Zhan Mahendra Rao
author_facet	Nasir Malik Xiantao Wang Sonia Shah Anastasia G Efthymiou Bin Yan Sabrina Heman-Ackah Ming Zhan Mahendra Rao
author_sort	Nasir Malik
title	Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
title_short	Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
title_full	Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
title_fullStr	Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
title_full_unstemmed	Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
title_sort	comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.
publisher	Public Library of Science (PLoS)
publishDate	2014
url	https://doaj.org/article/ee7c380eedef486580b14bf3ce02f19f
work_keys_str_mv	AT nasirmalik comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT xiantaowang comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT soniashah comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT anastasiagefthymiou comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT binyan comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT sabrinahemanackah comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT mingzhan comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes AT mahendrarao comparisonofthegeneexpressionprofilesofhumanfetalcorticalastrocyteswithpluripotentstemcellderivedneuralstemcellsidentifieshumanastrocytemarkersandsignalingpathwaysandtranscriptionfactorsactiveinhumanastrocytes
_version_	1718421958173917184

Comparison of the gene expression profiles of human fetal cortical astrocytes with pluripotent stem cell derived neural stem cells identifies human astrocyte markers and signaling pathways and transcription factors active in human astrocytes.

Ejemplares similares