A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
Ye Sun1, Yuzhao Tang1, Maoquan Chu2, Shuxian Song1, Yanfei Xin31School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, China; 2Institute of Life Science and Technology, Tongji University, Shanghai, China; 3School of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaAbstr...
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| Autores principales: | , , , , |
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| Formato: | article |
| Lenguaje: | EN |
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Dove Medical Press
2008
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| Acceso en línea: | https://doaj.org/article/ee8548eafb9c4fa79b82608ebf330d5a |
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| Sumario: | Ye Sun1, Yuzhao Tang1, Maoquan Chu2, Shuxian Song1, Yanfei Xin31School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, China; 2Institute of Life Science and Technology, Tongji University, Shanghai, China; 3School of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaAbstract: In order to quantify the amount of ligands or poly(ethylene glycol) (PEG) on each vector, here we developed a system in which poly-L-glutamic acid (PLG) was used as surface modification loading backbone, to which one PEG (MW 5000, 10000, 20000) or epidermal growth factor (EGF) was linked. The PLG conjugates can electro-statically adsorb upon DNA/polycation complex with positive charge, and, the amount of EGF or PEG on the surface of complexes could be varied. We have made a series of complexes containing the various PLG conjugates and examined their physicochemical properties, and made a comparison of properties and transfection efficiency between these complexes. EGF- and PEG-modified complexes showed 10–25-folds higher cell transfection efficiency than unmodified complexes in medium with or without serum.Keywords: gene delivery, poly(ethylene glycol), poly-L-glutamic acid, epidermal growing factor, poly-L-lysine |
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