A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery

A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery

Ye Sun1, Yuzhao Tang1, Maoquan Chu2, Shuxian Song1, Yanfei Xin31School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, China; 2Institute of Life Science and Technology, Tongji University, Shanghai, China; 3School of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaAbstr...

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Autores principales: Ye Sun, Yuzhao Tang, Maoquan Chu, Shuxian Song, Yanfei Xin
Formato: article
Lenguaje:EN
Publicado: Dove Medical Press 2008
Materias:
Medicine (General)
R5-920
Acceso en línea:https://doaj.org/article/ee8548eafb9c4fa79b82608ebf330d5a
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spelling oai:doaj.org-article:ee8548eafb9c4fa79b82608ebf330d5a2021-12-02T02:44:08ZA convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery1176-91141178-2013https://doaj.org/article/ee8548eafb9c4fa79b82608ebf330d5a2008-06-01T00:00:00Zhttp://www.dovepress.com/a-convenient-and-adjustable-surface-modified-complex-containing-poly-l-a1843https://doaj.org/toc/1176-9114https://doaj.org/toc/1178-2013Ye Sun1, Yuzhao Tang1, Maoquan Chu2, Shuxian Song1, Yanfei Xin31School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, China; 2Institute of Life Science and Technology, Tongji University, Shanghai, China; 3School of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaAbstract: In order to quantify the amount of ligands or poly(ethylene glycol) (PEG) on each vector, here we developed a system in which poly-L-glutamic acid (PLG) was used as surface modification loading backbone, to which one PEG (MW 5000, 10000, 20000) or epidermal growth factor (EGF) was linked. The PLG conjugates can electro-statically adsorb upon DNA/polycation complex with positive charge, and, the amount of EGF or PEG on the surface of complexes could be varied. We have made a series of complexes containing the various PLG conjugates and examined their physicochemical properties, and made a comparison of properties and transfection efficiency between these complexes. EGF- and PEG-modified complexes showed 10–25-folds higher cell transfection efficiency than unmodified complexes in medium with or without serum.Keywords: gene delivery, poly(ethylene glycol), poly-L-glutamic acid, epidermal growing factor, poly-L-lysine Ye SunYuzhao TangMaoquan ChuShuxian SongYanfei XinDove Medical PressarticleMedicine (General)R5-920ENInternational Journal of Nanomedicine, Vol 2008, Iss Issue 2, Pp 249-256 (2008)
institution DOAJ
collection DOAJ
language EN
topic Medicine (General)
R5-920
spellingShingle Medicine (General)
R5-920
Ye Sun
Yuzhao Tang
Maoquan Chu
Shuxian Song
Yanfei Xin
A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
description Ye Sun1, Yuzhao Tang1, Maoquan Chu2, Shuxian Song1, Yanfei Xin31School of Life Science and Technology, Shanghai Jiaotong University, Shanghai, China; 2Institute of Life Science and Technology, Tongji University, Shanghai, China; 3School of Pharmacy, Shanghai Jiaotong University, Shanghai, ChinaAbstract: In order to quantify the amount of ligands or poly(ethylene glycol) (PEG) on each vector, here we developed a system in which poly-L-glutamic acid (PLG) was used as surface modification loading backbone, to which one PEG (MW 5000, 10000, 20000) or epidermal growth factor (EGF) was linked. The PLG conjugates can electro-statically adsorb upon DNA/polycation complex with positive charge, and, the amount of EGF or PEG on the surface of complexes could be varied. We have made a series of complexes containing the various PLG conjugates and examined their physicochemical properties, and made a comparison of properties and transfection efficiency between these complexes. EGF- and PEG-modified complexes showed 10–25-folds higher cell transfection efficiency than unmodified complexes in medium with or without serum.Keywords: gene delivery, poly(ethylene glycol), poly-L-glutamic acid, epidermal growing factor, poly-L-lysine
format article
author Ye Sun
Yuzhao Tang
Maoquan Chu
Shuxian Song
Yanfei Xin
author_facet Ye Sun
Yuzhao Tang
Maoquan Chu
Shuxian Song
Yanfei Xin
author_sort Ye Sun
title A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
title_short A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
title_full A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
title_fullStr A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
title_full_unstemmed A convenient and adjustable surface-modified complex containing poly-L-glutamic acid conjugates as a vector for gene delivery
title_sort convenient and adjustable surface-modified complex containing poly-l-glutamic acid conjugates as a vector for gene delivery
publisher Dove Medical Press
publishDate 2008
url https://doaj.org/article/ee8548eafb9c4fa79b82608ebf330d5a
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