A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes

Abstract Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinat...

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Autores principales:	Eszter Toth, Sayed Muhammed Rassul, Martin Berry, Daniel Fulton
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee8adcc82dcb4e94a938ef11d7709eb1
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spelling	oai:doaj.org-article:ee8adcc82dcb4e94a938ef11d7709eb12021-12-02T16:51:49ZA morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes10.1038/s41598-021-88887-02045-2322https://doaj.org/article/ee8adcc82dcb4e94a938ef11d7709eb12021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-88887-0https://doaj.org/toc/2045-2322Abstract Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinating OL would advance our understanding of myelin plasticity and injury, thus we adapted a labelling approach involving Semliki Forest Virus (SFV) vectors to resolve and quantify the 3-D structure of OL processes and internodes in cerebellar slice cultures. We first demonstrate the utility of the approach by studying changes in OL morphology after complement-mediated injury. SFV vectors injected into cerebellar white matter labelled transitional OL (TOL), whose characteristic mixture of myelinating and non-myelinating processes exhibited significant degeneration after complement injury. The method was also capable of resolving finer changes in morphology related to neuronal activity. Prolonged suppression of neuronal activity, which reduced myelination, selectively decreased the length of putative internodes, and the proportion of process branches that supported them, while leaving other features of process morphology unaltered. Overall this work provides novel information on the morphology of TOL, and their response to conditions that alter circuit function or induce demyelination.Eszter TothSayed Muhammed RassulMartin BerryDaniel FultonNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-14 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Eszter Toth Sayed Muhammed Rassul Martin Berry Daniel Fulton A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
description	Abstract Neuronal activity is established as a driver of oligodendrocyte (OL) differentiation and myelination. The concept of activity-dependent myelin plasticity, and its role in cognition and disease, is gaining support. Methods capable of resolving changes in the morphology of individual myelinating OL would advance our understanding of myelin plasticity and injury, thus we adapted a labelling approach involving Semliki Forest Virus (SFV) vectors to resolve and quantify the 3-D structure of OL processes and internodes in cerebellar slice cultures. We first demonstrate the utility of the approach by studying changes in OL morphology after complement-mediated injury. SFV vectors injected into cerebellar white matter labelled transitional OL (TOL), whose characteristic mixture of myelinating and non-myelinating processes exhibited significant degeneration after complement injury. The method was also capable of resolving finer changes in morphology related to neuronal activity. Prolonged suppression of neuronal activity, which reduced myelination, selectively decreased the length of putative internodes, and the proportion of process branches that supported them, while leaving other features of process morphology unaltered. Overall this work provides novel information on the morphology of TOL, and their response to conditions that alter circuit function or induce demyelination.
format	article
author	Eszter Toth Sayed Muhammed Rassul Martin Berry Daniel Fulton
author_facet	Eszter Toth Sayed Muhammed Rassul Martin Berry Daniel Fulton
author_sort	Eszter Toth
title	A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
title_short	A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
title_full	A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
title_fullStr	A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
title_full_unstemmed	A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
title_sort	morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/ee8adcc82dcb4e94a938ef11d7709eb1
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A morphological analysis of activity-dependent myelination and myelin injury in transitional oligodendrocytes

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