Gene Expression Profiling of Skeletal Muscles

Next-generation sequencing provides an opportunity for an in-depth biocomputational analysis to identify gene expression patterns between soleus and tibialis anterior, two well-characterized skeletal muscles, and analyze their gene expression profiling. RNA read counts were analyzed for differential...

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Autores principales:	Sarah I. Alto, Chih-Ning Chang, Kevin Brown, Chrissa Kioussi, Theresa M. Filtz
Formato:	article
Lenguaje:	EN
Publicado:	MDPI AG 2021
Materias:	mouse skeletal muscle soleus tibialis anterior gene expression metabolism Genetics QH426-470
Acceso en línea:	https://doaj.org/article/ee8cfaba88f74fd19afa9fb273f99706
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id	oai:doaj.org-article:ee8cfaba88f74fd19afa9fb273f99706
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spelling	oai:doaj.org-article:ee8cfaba88f74fd19afa9fb273f997062021-11-25T17:41:13ZGene Expression Profiling of Skeletal Muscles10.3390/genes121117182073-4425https://doaj.org/article/ee8cfaba88f74fd19afa9fb273f997062021-10-01T00:00:00Zhttps://www.mdpi.com/2073-4425/12/11/1718https://doaj.org/toc/2073-4425Next-generation sequencing provides an opportunity for an in-depth biocomputational analysis to identify gene expression patterns between soleus and tibialis anterior, two well-characterized skeletal muscles, and analyze their gene expression profiling. RNA read counts were analyzed for differential gene expression using the R package edgeR. Differentially expressed genes were filtered using a false discovery rate of less than 0.05 c, a fold-change value of more than twenty, and an association with overrepresented pathways based on the Reactome pathway over-representation analysis tool. Most of the differentially expressed genes associated with soleus are coded for components of lipid metabolism and unique contractile elements. Differentially expressed genes associated with tibialis anterior encoded mostly for glucose and glycogen metabolic pathway regulatory enzymes and calcium-sensitive contractile components. These gene expression distinctions partly explain the genetic basis for skeletal muscle specialization, and they may help to explain skeletal muscle susceptibility to disease and drugs and further refine tissue engineering approaches.Sarah I. AltoChih-Ning ChangKevin BrownChrissa KioussiTheresa M. FiltzMDPI AGarticlemouseskeletal musclesoleustibialis anteriorgene expressionmetabolismGeneticsQH426-470ENGenes, Vol 12, Iss 1718, p 1718 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	mouse skeletal muscle soleus tibialis anterior gene expression metabolism Genetics QH426-470
spellingShingle	mouse skeletal muscle soleus tibialis anterior gene expression metabolism Genetics QH426-470 Sarah I. Alto Chih-Ning Chang Kevin Brown Chrissa Kioussi Theresa M. Filtz Gene Expression Profiling of Skeletal Muscles
description	Next-generation sequencing provides an opportunity for an in-depth biocomputational analysis to identify gene expression patterns between soleus and tibialis anterior, two well-characterized skeletal muscles, and analyze their gene expression profiling. RNA read counts were analyzed for differential gene expression using the R package edgeR. Differentially expressed genes were filtered using a false discovery rate of less than 0.05 c, a fold-change value of more than twenty, and an association with overrepresented pathways based on the Reactome pathway over-representation analysis tool. Most of the differentially expressed genes associated with soleus are coded for components of lipid metabolism and unique contractile elements. Differentially expressed genes associated with tibialis anterior encoded mostly for glucose and glycogen metabolic pathway regulatory enzymes and calcium-sensitive contractile components. These gene expression distinctions partly explain the genetic basis for skeletal muscle specialization, and they may help to explain skeletal muscle susceptibility to disease and drugs and further refine tissue engineering approaches.
format	article
author	Sarah I. Alto Chih-Ning Chang Kevin Brown Chrissa Kioussi Theresa M. Filtz
author_facet	Sarah I. Alto Chih-Ning Chang Kevin Brown Chrissa Kioussi Theresa M. Filtz
author_sort	Sarah I. Alto
title	Gene Expression Profiling of Skeletal Muscles
title_short	Gene Expression Profiling of Skeletal Muscles
title_full	Gene Expression Profiling of Skeletal Muscles
title_fullStr	Gene Expression Profiling of Skeletal Muscles
title_full_unstemmed	Gene Expression Profiling of Skeletal Muscles
title_sort	gene expression profiling of skeletal muscles
publisher	MDPI AG
publishDate	2021
url	https://doaj.org/article/ee8cfaba88f74fd19afa9fb273f99706
work_keys_str_mv	AT sarahialto geneexpressionprofilingofskeletalmuscles AT chihningchang geneexpressionprofilingofskeletalmuscles AT kevinbrown geneexpressionprofilingofskeletalmuscles AT chrissakioussi geneexpressionprofilingofskeletalmuscles AT theresamfiltz geneexpressionprofilingofskeletalmuscles
_version_	1718412139539988480

Gene Expression Profiling of Skeletal Muscles

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