Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients

Abstract. Surniyantoro HNE, Rahajeng N, Lusiyanti Y, Rahardjo T, Erawati D, Choridah L, Dhamiyati W, Dwidanarti SR. 2019. Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients. Biodiversitas 20: 2128-2133. Two of the common single-nucleotide polymorphisms are...

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Autores principales:	HARRY NUGROHO EKO SURNIYANTORO, NASTITI RAHAJENG, YANTI LUSIYANTI, TUR RAHARDJO, DYAH ERAWATI, LINA CHORIDAH, WIGATI DHAMIYATI, SRI RETNA DWIDANARTI
Formato:	article
Lenguaje:	EN
Publicado:	MBI & UNS Solo 2019
Materias:	194trp, 399gln, micronuclei, radiation, cancer patients Biology (General) QH301-705.5
Acceso en línea:	https://doaj.org/article/ee9185833a2843dc92611f52159b96b5
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spelling	oai:doaj.org-article:ee9185833a2843dc92611f52159b96b52021-11-21T21:43:55ZInteraction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients1412-033X2085-472210.13057/biodiv/d200805https://doaj.org/article/ee9185833a2843dc92611f52159b96b52019-07-01T00:00:00Zhttps://smujo.id/biodiv/article/view/3897https://doaj.org/toc/1412-033Xhttps://doaj.org/toc/2085-4722Abstract. Surniyantoro HNE, Rahajeng N, Lusiyanti Y, Rahardjo T, Erawati D, Choridah L, Dhamiyati W, Dwidanarti SR. 2019. Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients. Biodiversitas 20: 2128-2133. Two of the common single-nucleotide polymorphisms are X-ray repair cross-complementary group 1 on exon 6 (Arg194Trp) and exon 10 (Arg399Gln). The purpose of this study was to determine the interactions between Arg194Trp and Arg399Gln genotypes combination with the risk of radiation on cancer patients in Indonesia, linked to micronuclei frequency as a biomarker of DNA damage. This study consisted of 19 patients with various cancer as the case group and 37 non-cancer participants as the control group. The determination of Arg149Trp and Arg399Gln alleles were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Micronuclei assay was performed using Cytokinesis-block micronuclei cytome assay. The results of our study showed that micronuclei frequency was very significantly higher in the cancer patients compared to controls (111.16 ± 76.24 versus 16.89 ± 9.72, p<0.0001). Patients with heterozygous mutant genotypes CT had a lower frequency of micronuclei than patients with normal CC genotypes (105.6 ± 80.97 versus 117.33 ± 74.97). Likewise, patients with mutant genotype AA had a lower frequency of micronuclei than patients with normal GG genotype (64 versus 129.71 ± 90.68). The genetic polymorphisms of Arg194Trp and Arg399Gln demonstrated an association with the level of DNA damage on cancer patients.HARRY NUGROHO EKO SURNIYANTORONASTITI RAHAJENGYANTI LUSIYANTITUR RAHARDJODYAH ERAWATILINA CHORIDAHWIGATI DHAMIYATISRI RETNA DWIDANARTIMBI & UNS Soloarticle194trp, 399gln, micronuclei, radiation, cancer patientsBiology (General)QH301-705.5ENBiodiversitas, Vol 20, Iss 8 (2019)
institution	DOAJ
collection	DOAJ
language	EN
topic	194trp, 399gln, micronuclei, radiation, cancer patients Biology (General) QH301-705.5
spellingShingle	194trp, 399gln, micronuclei, radiation, cancer patients Biology (General) QH301-705.5 HARRY NUGROHO EKO SURNIYANTORO NASTITI RAHAJENG YANTI LUSIYANTI TUR RAHARDJO DYAH ERAWATI LINA CHORIDAH WIGATI DHAMIYATI SRI RETNA DWIDANARTI Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients
description	Abstract. Surniyantoro HNE, Rahajeng N, Lusiyanti Y, Rahardjo T, Erawati D, Choridah L, Dhamiyati W, Dwidanarti SR. 2019. Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients. Biodiversitas 20: 2128-2133. Two of the common single-nucleotide polymorphisms are X-ray repair cross-complementary group 1 on exon 6 (Arg194Trp) and exon 10 (Arg399Gln). The purpose of this study was to determine the interactions between Arg194Trp and Arg399Gln genotypes combination with the risk of radiation on cancer patients in Indonesia, linked to micronuclei frequency as a biomarker of DNA damage. This study consisted of 19 patients with various cancer as the case group and 37 non-cancer participants as the control group. The determination of Arg149Trp and Arg399Gln alleles were performed using Polymerase Chain Reaction-Restriction Fragment Length Polymorphism. Micronuclei assay was performed using Cytokinesis-block micronuclei cytome assay. The results of our study showed that micronuclei frequency was very significantly higher in the cancer patients compared to controls (111.16 ± 76.24 versus 16.89 ± 9.72, p<0.0001). Patients with heterozygous mutant genotypes CT had a lower frequency of micronuclei than patients with normal CC genotypes (105.6 ± 80.97 versus 117.33 ± 74.97). Likewise, patients with mutant genotype AA had a lower frequency of micronuclei than patients with normal GG genotype (64 versus 129.71 ± 90.68). The genetic polymorphisms of Arg194Trp and Arg399Gln demonstrated an association with the level of DNA damage on cancer patients.
format	article
author	HARRY NUGROHO EKO SURNIYANTORO NASTITI RAHAJENG YANTI LUSIYANTI TUR RAHARDJO DYAH ERAWATI LINA CHORIDAH WIGATI DHAMIYATI SRI RETNA DWIDANARTI
author_facet	HARRY NUGROHO EKO SURNIYANTORO NASTITI RAHAJENG YANTI LUSIYANTI TUR RAHARDJO DYAH ERAWATI LINA CHORIDAH WIGATI DHAMIYATI SRI RETNA DWIDANARTI
author_sort	HARRY NUGROHO EKO SURNIYANTORO
title	Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients
title_short	Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients
title_full	Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients
title_fullStr	Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients
title_full_unstemmed	Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients
title_sort	interaction of arg194trp and arg399gln genotypes with the risk of radiation on cancer patients
publisher	MBI & UNS Solo
publishDate	2019
url	https://doaj.org/article/ee9185833a2843dc92611f52159b96b5
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Interaction of Arg194Trp and Arg399Gln genotypes with the risk of radiation on cancer patients

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