Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples

Analysis of chloramphenicol (CL) and hydrocortisone acetate (HCA) in cream samples using FTIR (Fourier Transform Infrared) spectroscopy. The objective of this study was to develop FTIR spectroscopy combined with multivariate calibrations for effective analysis of CL and HCA in cream formulation. Hig...

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Autores principales:	Kusnul Khotimah, Sudibyo Martono, Anjar Windarsih, Irnawati Irnawati, Erna Prihandiwati, Abdul Rohman
Formato:	article
Lenguaje:	EN
Publicado:	Universitas Gadjah Mada 2021
Materias:	chloramphenicol hydrocortisone acetate ftir spectroscopy partial least square cream Pharmacy and materia medica RS1-441
Acceso en línea:	https://doaj.org/article/ee935175c28b46b0994239f209d38c46
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spelling	oai:doaj.org-article:ee935175c28b46b0994239f209d38c462021-11-15T06:08:46ZApplication of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples2338-94272338-9486https://doaj.org/article/ee935175c28b46b0994239f209d38c462021-09-01T00:00:00Zhttps://jurnal.ugm.ac.id/v3/IJP/article/view/2687https://doaj.org/toc/2338-9427https://doaj.org/toc/2338-9486Analysis of chloramphenicol (CL) and hydrocortisone acetate (HCA) in cream samples using FTIR (Fourier Transform Infrared) spectroscopy. The objective of this study was to develop FTIR spectroscopy combined with multivariate calibrations for effective analysis of CL and HCA in cream formulation. High performance liquid chromatography (HPLC) method was applied for determining the actual value of CL and HCA. Cream samples containing CL and HCA were scanned using FTIR spectrophotometer employing attenuated total reflectance (ATR) technique. FTIR-ATR spectra were subjected to several optimization including wavenumbers selection and derivatization for quantitative analysis. The results showed that the optimum prediction model for correlation between actual values of CL and HCA as determined by HPLC and FTIR-predicted values was obtained using first derivative FTIR spectra at wavenumbers of 1500-1000 cm-1. The R2 value for calibration and internal validation for CL and HCA was > 0.9 with relatively small RMSEC (root mean square error of calibrations), RMSECV (root mean square error of cross validations) and PRESS (predicted residual error sum of squares) values. External validation of CL and HCA models produced R2 > 0.98 with RMSEP values of 0.6501 and 1.0041, respectively. The results of CL and HCA assay using HPLC and FTIR spectroscopy were compared resulting non-significant results (p > 0.05) for these two methods. FTIR spectroscopy in combination with PLS can be used as a rapid and reliable analytical method for determination of CL and HCA in cream formulation.Kusnul KhotimahSudibyo MartonoAnjar WindarsihIrnawati IrnawatiErna PrihandiwatiAbdul RohmanUniversitas Gadjah Madaarticlechloramphenicolhydrocortisone acetateftir spectroscopypartial least squarecreamPharmacy and materia medicaRS1-441ENIndonesian Journal of Pharmacy, Pp 408-415 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	chloramphenicol hydrocortisone acetate ftir spectroscopy partial least square cream Pharmacy and materia medica RS1-441
spellingShingle	chloramphenicol hydrocortisone acetate ftir spectroscopy partial least square cream Pharmacy and materia medica RS1-441 Kusnul Khotimah Sudibyo Martono Anjar Windarsih Irnawati Irnawati Erna Prihandiwati Abdul Rohman Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples
description	Analysis of chloramphenicol (CL) and hydrocortisone acetate (HCA) in cream samples using FTIR (Fourier Transform Infrared) spectroscopy. The objective of this study was to develop FTIR spectroscopy combined with multivariate calibrations for effective analysis of CL and HCA in cream formulation. High performance liquid chromatography (HPLC) method was applied for determining the actual value of CL and HCA. Cream samples containing CL and HCA were scanned using FTIR spectrophotometer employing attenuated total reflectance (ATR) technique. FTIR-ATR spectra were subjected to several optimization including wavenumbers selection and derivatization for quantitative analysis. The results showed that the optimum prediction model for correlation between actual values of CL and HCA as determined by HPLC and FTIR-predicted values was obtained using first derivative FTIR spectra at wavenumbers of 1500-1000 cm-1. The R2 value for calibration and internal validation for CL and HCA was > 0.9 with relatively small RMSEC (root mean square error of calibrations), RMSECV (root mean square error of cross validations) and PRESS (predicted residual error sum of squares) values. External validation of CL and HCA models produced R2 > 0.98 with RMSEP values of 0.6501 and 1.0041, respectively. The results of CL and HCA assay using HPLC and FTIR spectroscopy were compared resulting non-significant results (p > 0.05) for these two methods. FTIR spectroscopy in combination with PLS can be used as a rapid and reliable analytical method for determination of CL and HCA in cream formulation.
format	article
author	Kusnul Khotimah Sudibyo Martono Anjar Windarsih Irnawati Irnawati Erna Prihandiwati Abdul Rohman
author_facet	Kusnul Khotimah Sudibyo Martono Anjar Windarsih Irnawati Irnawati Erna Prihandiwati Abdul Rohman
author_sort	Kusnul Khotimah
title	Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples
title_short	Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples
title_full	Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples
title_fullStr	Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples
title_full_unstemmed	Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples
title_sort	application of ftir spectroscopy combined with multivariate calibrations for analysis of chloramphenicol and hydrocortisone acetate in cream samples
publisher	Universitas Gadjah Mada
publishDate	2021
url	https://doaj.org/article/ee935175c28b46b0994239f209d38c46
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Application of FTIR Spectroscopy Combined with Multivariate Calibrations for Analysis of Chloramphenicol and Hydrocortisone Acetate in Cream Samples

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