Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.

Dendritic cells have been investigated in clinical trials, predominantly with the aim of stimulating immune responses against tumours or infectious diseases. Thus far, however, no clinical studies have taken advantage of their specific immunosuppressive potential. Tolerogenic DCs may represent a new...

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Autores principales:	Raquel Cabezón, Elena Ricart, Carolina España, Julián Panés, Daniel Benitez-Ribas
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2012
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/ee96cdf8f79f412aaa9eb747b67fb428
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spelling	oai:doaj.org-article:ee96cdf8f79f412aaa9eb747b67fb4282021-11-18T08:03:30ZGram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.1932-620310.1371/journal.pone.0052456https://doaj.org/article/ee96cdf8f79f412aaa9eb747b67fb4282012-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/23300676/?tool=EBIhttps://doaj.org/toc/1932-6203Dendritic cells have been investigated in clinical trials, predominantly with the aim of stimulating immune responses against tumours or infectious diseases. Thus far, however, no clinical studies have taken advantage of their specific immunosuppressive potential. Tolerogenic DCs may represent a new therapeutic strategy for human immune-based diseases, such as Crohn's disease, where the perturbations of the finely tuned balance between the immune system and the microflora result in disease. In the present report, we describe the generation of tolerogenic DCs from healthy donors and Crohn's disease patients using clinical-grade reagents in combination with dexamethasone as immunosuppressive agent and characterize their response to maturation stimuli. Interestingly, we found out that dexamethasone-conditioned DCs keep their tolerogenic properties to Gram-negative bacteria. Other findings included in this study demonstrate that the combination of dexamethasone with a specific cytokine cocktail yielded clinical-grade DCs with the following characteristics: a semi-mature phenotype, a pronounced shift towards anti-inflammatory versus inflammatory cytokine production and low T-cell stimulatory properties. Importantly, in regard to their clinical application, the tolerogenic phenotype of DCs remained stable after the elimination of dexamethasone and after a second stimulation with LPS or bacteria. All these properties make this cell product suitable to be tested in clinical trials of inflammatory conditions including Crohn's disease.Raquel CabezónElena RicartCarolina EspañaJulián PanésDaniel Benitez-RibasPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 7, Iss 12, p e52456 (2012)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Raquel Cabezón Elena Ricart Carolina España Julián Panés Daniel Benitez-Ribas Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
description	Dendritic cells have been investigated in clinical trials, predominantly with the aim of stimulating immune responses against tumours or infectious diseases. Thus far, however, no clinical studies have taken advantage of their specific immunosuppressive potential. Tolerogenic DCs may represent a new therapeutic strategy for human immune-based diseases, such as Crohn's disease, where the perturbations of the finely tuned balance between the immune system and the microflora result in disease. In the present report, we describe the generation of tolerogenic DCs from healthy donors and Crohn's disease patients using clinical-grade reagents in combination with dexamethasone as immunosuppressive agent and characterize their response to maturation stimuli. Interestingly, we found out that dexamethasone-conditioned DCs keep their tolerogenic properties to Gram-negative bacteria. Other findings included in this study demonstrate that the combination of dexamethasone with a specific cytokine cocktail yielded clinical-grade DCs with the following characteristics: a semi-mature phenotype, a pronounced shift towards anti-inflammatory versus inflammatory cytokine production and low T-cell stimulatory properties. Importantly, in regard to their clinical application, the tolerogenic phenotype of DCs remained stable after the elimination of dexamethasone and after a second stimulation with LPS or bacteria. All these properties make this cell product suitable to be tested in clinical trials of inflammatory conditions including Crohn's disease.
format	article
author	Raquel Cabezón Elena Ricart Carolina España Julián Panés Daniel Benitez-Ribas
author_facet	Raquel Cabezón Elena Ricart Carolina España Julián Panés Daniel Benitez-Ribas
author_sort	Raquel Cabezón
title	Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
title_short	Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
title_full	Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
title_fullStr	Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
title_full_unstemmed	Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
title_sort	gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.
publisher	Public Library of Science (PLoS)
publishDate	2012
url	https://doaj.org/article/ee96cdf8f79f412aaa9eb747b67fb428
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_version_	1718422639923429376

Gram-negative enterobacteria induce tolerogenic maturation in dexamethasone conditioned dendritic cells.

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