Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

Summary: The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includ...

Descripción completa

Guardado en:

Detalles Bibliográficos
Autores principales:	Xinzheng Guo, Christopher Starr, Jing Zhou, Bo Chen
Formato:	article
Lenguaje:	EN
Publicado:	Elsevier 2021
Materias:	Cell Biology Cell-based Assays Microscopy Model Organisms Gene Expression Neuroscience Science (General) Q1-390
Acceso en línea:	https://doaj.org/article/ee9d4001b61248c8ab7cf156f4f4e35f
Etiquetas:	Agregar Etiqueta Sin Etiquetas, Sea el primero en etiquetar este registro!

id	oai:doaj.org-article:ee9d4001b61248c8ab7cf156f4f4e35f
record_format	dspace
spelling	oai:doaj.org-article:ee9d4001b61248c8ab7cf156f4f4e35f2021-11-06T04:36:01ZProtocol for evaluating the role of a gene in protecting mouse retinal ganglion cells2666-166710.1016/j.xpro.2021.100932https://doaj.org/article/ee9d4001b61248c8ab7cf156f4f4e35f2021-12-01T00:00:00Zhttp://www.sciencedirect.com/science/article/pii/S2666166721006389https://doaj.org/toc/2666-1667Summary: The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival.For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).Xinzheng GuoChristopher StarrJing ZhouBo ChenElsevierarticleCell BiologyCell-based AssaysMicroscopyModel OrganismsGene ExpressionNeuroscienceScience (General)Q1-390ENSTAR Protocols, Vol 2, Iss 4, Pp 100932- (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Cell Biology Cell-based Assays Microscopy Model Organisms Gene Expression Neuroscience Science (General) Q1-390
spellingShingle	Cell Biology Cell-based Assays Microscopy Model Organisms Gene Expression Neuroscience Science (General) Q1-390 Xinzheng Guo Christopher Starr Jing Zhou Bo Chen Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
description	Summary: The degeneration of retinal ganglion cells (RGCs) leads to irreversible vision loss in a variety of pathological states. Here, we describe a protocol to evaluate the role of a gene in protecting mouse RGCs when they sustain injuries from excitotoxicity or axonal damage. This protocol includes the procedures for gene transfer through AAV intravitreal injection, induction of RGC injuries by NMDA-induced excitotoxicity or optic nerve crush, and retina immunohistochemistry to assess RGC survival.For complete details on the use and execution of this protocol, please refer to Guo et al. (2021).
format	article
author	Xinzheng Guo Christopher Starr Jing Zhou Bo Chen
author_facet	Xinzheng Guo Christopher Starr Jing Zhou Bo Chen
author_sort	Xinzheng Guo
title	Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_short	Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_full	Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_fullStr	Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_full_unstemmed	Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
title_sort	protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells
publisher	Elsevier
publishDate	2021
url	https://doaj.org/article/ee9d4001b61248c8ab7cf156f4f4e35f
work_keys_str_mv	AT xinzhengguo protocolforevaluatingtheroleofageneinprotectingmouseretinalganglioncells AT christopherstarr protocolforevaluatingtheroleofageneinprotectingmouseretinalganglioncells AT jingzhou protocolforevaluatingtheroleofageneinprotectingmouseretinalganglioncells AT bochen protocolforevaluatingtheroleofageneinprotectingmouseretinalganglioncells
_version_	1718443892253130752

Protocol for evaluating the role of a gene in protecting mouse retinal ganglion cells

Ejemplares similares