Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo

Abstract The excessive release and accumulation of glutamate in the brain is known to be associated with excitotoxicity. CE, an extract derived from the plant Coeloglossum viride var. Bracteatum, exerted neuroprotective effects against amyloid toxicity and oxidative stress in cortical neurons. The a...

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Autores principales:	Rui-Yuan Pan, Jun Ma, Huan-Tong Wu, Qing-Shan Liu, Xiao-Yan Qin, Yong Cheng
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2017
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eead284456304c33b54ffb274ee87f85
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spelling	oai:doaj.org-article:eead284456304c33b54ffb274ee87f852021-12-02T16:07:59ZNeuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo10.1038/s41598-017-08957-02045-2322https://doaj.org/article/eead284456304c33b54ffb274ee87f852017-08-01T00:00:00Zhttps://doi.org/10.1038/s41598-017-08957-0https://doaj.org/toc/2045-2322Abstract The excessive release and accumulation of glutamate in the brain is known to be associated with excitotoxicity. CE, an extract derived from the plant Coeloglossum viride var. Bracteatum, exerted neuroprotective effects against amyloid toxicity and oxidative stress in cortical neurons. The aims of this study are to examine whether CE also attenuates glutamate neurotoxicity in rat primary cultured cortical neurons and to determine the effect of CE in vivo. According to the results of MTT, LDH release, and TUNEL assays, the CE treatment significantly reduced glutamate-induced neurotoxicity in a dose-dependent manner. Moreover, the protective effects of CE were blocked by an Akt inhibitor, LY294002, suggesting that the PI3K/Akt signalling pathway is involved in the neuroprotective effects of CE. In addition, CE might regulate the PKC-GluA2 axis to prevent neuronal apoptosis. CE also protected against dopaminergic neuronal loss in a mouse model of MPTP-induced PD. Based on our results, CE exerted neuroprotective effects both in vitro and in vivo, thus providing a potential therapeutic target for the treatment or prevention of neurodegeneration.Rui-Yuan PanJun MaHuan-Tong WuQing-Shan LiuXiao-Yan QinYong ChengNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 7, Iss 1, Pp 1-9 (2017)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Rui-Yuan Pan Jun Ma Huan-Tong Wu Qing-Shan Liu Xiao-Yan Qin Yong Cheng Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo
description	Abstract The excessive release and accumulation of glutamate in the brain is known to be associated with excitotoxicity. CE, an extract derived from the plant Coeloglossum viride var. Bracteatum, exerted neuroprotective effects against amyloid toxicity and oxidative stress in cortical neurons. The aims of this study are to examine whether CE also attenuates glutamate neurotoxicity in rat primary cultured cortical neurons and to determine the effect of CE in vivo. According to the results of MTT, LDH release, and TUNEL assays, the CE treatment significantly reduced glutamate-induced neurotoxicity in a dose-dependent manner. Moreover, the protective effects of CE were blocked by an Akt inhibitor, LY294002, suggesting that the PI3K/Akt signalling pathway is involved in the neuroprotective effects of CE. In addition, CE might regulate the PKC-GluA2 axis to prevent neuronal apoptosis. CE also protected against dopaminergic neuronal loss in a mouse model of MPTP-induced PD. Based on our results, CE exerted neuroprotective effects both in vitro and in vivo, thus providing a potential therapeutic target for the treatment or prevention of neurodegeneration.
format	article
author	Rui-Yuan Pan Jun Ma Huan-Tong Wu Qing-Shan Liu Xiao-Yan Qin Yong Cheng
author_facet	Rui-Yuan Pan Jun Ma Huan-Tong Wu Qing-Shan Liu Xiao-Yan Qin Yong Cheng
author_sort	Rui-Yuan Pan
title	Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo
title_short	Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo
title_full	Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo
title_fullStr	Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo
title_full_unstemmed	Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo
title_sort	neuroprotective effects of a coeloglossum viride var. bracteatum extract in vitro and in vivo
publisher	Nature Portfolio
publishDate	2017
url	https://doaj.org/article/eead284456304c33b54ffb274ee87f85
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Neuroprotective effects of a Coeloglossum viride var. Bracteatum extract in vitro and in vivo

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