Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain
Objectives: The study aimed to characterize the clonal spread of resistant bacteria and dissemination of resistance plasmids among carbapenem-resistant Enterobacterales at a tertiary hospital in Catalonia, Spain.Methods: Isolates were recovered from surveillance rectal swabs and diagnostic samples....
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Frontiers Media S.A.
2021
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oai:doaj.org-article:eeaeb15bc67546459cc47cb1421d2c682021-11-18T07:12:26ZClonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain1664-302X10.3389/fmicb.2021.781127https://doaj.org/article/eeaeb15bc67546459cc47cb1421d2c682021-11-01T00:00:00Zhttps://www.frontiersin.org/articles/10.3389/fmicb.2021.781127/fullhttps://doaj.org/toc/1664-302XObjectives: The study aimed to characterize the clonal spread of resistant bacteria and dissemination of resistance plasmids among carbapenem-resistant Enterobacterales at a tertiary hospital in Catalonia, Spain.Methods: Isolates were recovered from surveillance rectal swabs and diagnostic samples. Species identification was by matrix-assisted laser desorption ionization-time time of flight mass spectrometry (MALDI-TOF MS). Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Antimicrobial susceptibility was assessed by gradient-diffusion and carriage of bla genes was detected by PCR. Plasmid typing, conjugation assays, S1-PFGE studies and long-read sequencing were used to characterize resistance plasmids.Results: From July 2018 to February 2019, 125 Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales were recovered from 101 inpatients from surveillance (74.4%) or clinical samples (25.6%), in a tertiary hospital in Barcelona. Clonality studies identified a major clone of Klebsiella pneumoniae belonging to sequence type ST15 and additional isolates of K. pneumoniae, Escherichia coli and Enterobacter sp. from different STs. All isolates but one carried the blaKPC–2 allelic variant. The blaKPC–2 gene was located in an IncFIIk plasmid of circa 106 Kb in a non-classical Tn4401 element designated NTEKPC-pMC-2-1. Whole-genome sequencing revealed different rearrangements of the 106 Kb plasmid while the NTEKPC-pMC-2-1 module was highly conserved.Conclusion: We report a hospital outbreak caused by the clonal dissemination of KPC-producing ST15 K. pneumoniae but also the intra- and inter-species transmission of the blaKPC–2 gene associated with plasmid conjugation and/or transposon dissemination. To our knowledge, this is the first report of an outbreak caused by KPC-producing Enterobacterales isolated from human patients in Catalonia and highlights the relevance of surveillance studies in the early detection and control of antibiotic resistant high-risk clones.Marta Marí-AlmirallNúria FerrandoMariana José FernándezClara CosgayaJoaquim ViñesJoaquim ViñesElisa RubioAnna CuscóLaura MuñozMartina PelliceAndrea VergaraIrene CampoLaura Rodríguez-SernaGemina SantanaAna Del RíoOlga FrancinoPilar CiruelaPilar CiruelaFrederic BallesterFrancesc MarcoFrancesc MarcoJosé Antonio MartínezÁlex SorianoCristina PitartCristina PitartJordi VilaJordi VilaIgnasi RocaMERCyCAT Study GroupPepa Pérez JoveEmma PadillaMónica Ballestero-TéllezYuliya ZboromyrskaMiguel Ángel BenítezRaquel ClivilléSabina GonzálezIolanda CalvetCarmen GallésGoretti SaucaCarmina Martí-SalaMᵃ Angeles PulidoAnna VilamalaAraceli González-CuevasAmadeu GenéGloria TrujilloJoan Lopez MadueñoXavier RagaFrederic Gómez Ester PicóCarolina SarviséIsabel PujolXesca FontFrontiers Media S.A.articleKlebsiellaoutbreakantibiotic resistancecarbapenemaseepidemiologyplasmidMicrobiologyQR1-502ENFrontiers in Microbiology, Vol 12 (2021) |
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DOAJ |
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EN |
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Klebsiella outbreak antibiotic resistance carbapenemase epidemiology plasmid Microbiology QR1-502 |
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Klebsiella outbreak antibiotic resistance carbapenemase epidemiology plasmid Microbiology QR1-502 Marta Marí-Almirall Núria Ferrando Mariana José Fernández Clara Cosgaya Joaquim Viñes Joaquim Viñes Elisa Rubio Anna Cuscó Laura Muñoz Martina Pellice Andrea Vergara Irene Campo Laura Rodríguez-Serna Gemina Santana Ana Del Río Olga Francino Pilar Ciruela Pilar Ciruela Frederic Ballester Francesc Marco Francesc Marco José Antonio Martínez Álex Soriano Cristina Pitart Cristina Pitart Jordi Vila Jordi Vila Ignasi Roca MERCyCAT Study Group Pepa Pérez Jove Emma Padilla Mónica Ballestero-Téllez Yuliya Zboromyrska Miguel Ángel Benítez Raquel Clivillé Sabina González Iolanda Calvet Carmen Gallés Goretti Sauca Carmina Martí-Sala Mᵃ Angeles Pulido Anna Vilamala Araceli González-Cuevas Amadeu Gené Gloria Trujillo Joan Lopez Madueño Xavier Raga Frederic Gómez Ester Picó Carolina Sarvisé Isabel Pujol Xesca Font Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain |
| description |
Objectives: The study aimed to characterize the clonal spread of resistant bacteria and dissemination of resistance plasmids among carbapenem-resistant Enterobacterales at a tertiary hospital in Catalonia, Spain.Methods: Isolates were recovered from surveillance rectal swabs and diagnostic samples. Species identification was by matrix-assisted laser desorption ionization-time time of flight mass spectrometry (MALDI-TOF MS). Molecular typing was performed by pulsed-field gel electrophoresis (PFGE) and multi-locus sequence typing (MLST). Antimicrobial susceptibility was assessed by gradient-diffusion and carriage of bla genes was detected by PCR. Plasmid typing, conjugation assays, S1-PFGE studies and long-read sequencing were used to characterize resistance plasmids.Results: From July 2018 to February 2019, 125 Klebsiella pneumoniae carbapenemase (KPC)-producing Enterobacterales were recovered from 101 inpatients from surveillance (74.4%) or clinical samples (25.6%), in a tertiary hospital in Barcelona. Clonality studies identified a major clone of Klebsiella pneumoniae belonging to sequence type ST15 and additional isolates of K. pneumoniae, Escherichia coli and Enterobacter sp. from different STs. All isolates but one carried the blaKPC–2 allelic variant. The blaKPC–2 gene was located in an IncFIIk plasmid of circa 106 Kb in a non-classical Tn4401 element designated NTEKPC-pMC-2-1. Whole-genome sequencing revealed different rearrangements of the 106 Kb plasmid while the NTEKPC-pMC-2-1 module was highly conserved.Conclusion: We report a hospital outbreak caused by the clonal dissemination of KPC-producing ST15 K. pneumoniae but also the intra- and inter-species transmission of the blaKPC–2 gene associated with plasmid conjugation and/or transposon dissemination. To our knowledge, this is the first report of an outbreak caused by KPC-producing Enterobacterales isolated from human patients in Catalonia and highlights the relevance of surveillance studies in the early detection and control of antibiotic resistant high-risk clones. |
| format |
article |
| author |
Marta Marí-Almirall Núria Ferrando Mariana José Fernández Clara Cosgaya Joaquim Viñes Joaquim Viñes Elisa Rubio Anna Cuscó Laura Muñoz Martina Pellice Andrea Vergara Irene Campo Laura Rodríguez-Serna Gemina Santana Ana Del Río Olga Francino Pilar Ciruela Pilar Ciruela Frederic Ballester Francesc Marco Francesc Marco José Antonio Martínez Álex Soriano Cristina Pitart Cristina Pitart Jordi Vila Jordi Vila Ignasi Roca MERCyCAT Study Group Pepa Pérez Jove Emma Padilla Mónica Ballestero-Téllez Yuliya Zboromyrska Miguel Ángel Benítez Raquel Clivillé Sabina González Iolanda Calvet Carmen Gallés Goretti Sauca Carmina Martí-Sala Mᵃ Angeles Pulido Anna Vilamala Araceli González-Cuevas Amadeu Gené Gloria Trujillo Joan Lopez Madueño Xavier Raga Frederic Gómez Ester Picó Carolina Sarvisé Isabel Pujol Xesca Font |
| author_facet |
Marta Marí-Almirall Núria Ferrando Mariana José Fernández Clara Cosgaya Joaquim Viñes Joaquim Viñes Elisa Rubio Anna Cuscó Laura Muñoz Martina Pellice Andrea Vergara Irene Campo Laura Rodríguez-Serna Gemina Santana Ana Del Río Olga Francino Pilar Ciruela Pilar Ciruela Frederic Ballester Francesc Marco Francesc Marco José Antonio Martínez Álex Soriano Cristina Pitart Cristina Pitart Jordi Vila Jordi Vila Ignasi Roca MERCyCAT Study Group Pepa Pérez Jove Emma Padilla Mónica Ballestero-Téllez Yuliya Zboromyrska Miguel Ángel Benítez Raquel Clivillé Sabina González Iolanda Calvet Carmen Gallés Goretti Sauca Carmina Martí-Sala Mᵃ Angeles Pulido Anna Vilamala Araceli González-Cuevas Amadeu Gené Gloria Trujillo Joan Lopez Madueño Xavier Raga Frederic Gómez Ester Picó Carolina Sarvisé Isabel Pujol Xesca Font |
| author_sort |
Marta Marí-Almirall |
| title |
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain |
| title_short |
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain |
| title_full |
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain |
| title_fullStr |
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain |
| title_full_unstemmed |
Clonal Spread and Intra- and Inter-Species Plasmid Dissemination Associated With Klebsiella pneumoniae Carbapenemase-Producing Enterobacterales During a Hospital Outbreak in Barcelona, Spain |
| title_sort |
clonal spread and intra- and inter-species plasmid dissemination associated with klebsiella pneumoniae carbapenemase-producing enterobacterales during a hospital outbreak in barcelona, spain |
| publisher |
Frontiers Media S.A. |
| publishDate |
2021 |
| url |
https://doaj.org/article/eeaeb15bc67546459cc47cb1421d2c68 |
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