Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses

Abstract NACs are plant-specific transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NAC-SAGs belong t...

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Autores principales:	Bruno Paes Melo, Isabela Tristan Lourenço-Tessutti, Otto Teixeira Fraga, Luanna Bezerra Pinheiro, Camila Barrozo de Jesus Lins, Carolina Vianna Morgante, Janice Almeida Engler, Pedro Augusto Braga Reis, Maria Fátima Grossi-de-Sá, Elizabeth Pacheco Batista Fontes
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eeaf472185ca4f26aca654521a107d93
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spelling	oai:doaj.org-article:eeaf472185ca4f26aca654521a107d932021-12-02T14:49:10ZContrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses10.1038/s41598-021-90767-62045-2322https://doaj.org/article/eeaf472185ca4f26aca654521a107d932021-05-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-90767-6https://doaj.org/toc/2045-2322Abstract NACs are plant-specific transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NAC-SAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally different gene sets involved in stress and PCD, and (iii) are differentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and GmNAC065 regulators function inversely in developmental and environmental PCD.Bruno Paes MeloIsabela Tristan Lourenço-TessuttiOtto Teixeira FragaLuanna Bezerra PinheiroCamila Barrozo de Jesus LinsCarolina Vianna MorganteJanice Almeida EnglerPedro Augusto Braga ReisMaria Fátima Grossi-de-SáElizabeth Pacheco Batista FontesNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-21 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Bruno Paes Melo Isabela Tristan Lourenço-Tessutti Otto Teixeira Fraga Luanna Bezerra Pinheiro Camila Barrozo de Jesus Lins Carolina Vianna Morgante Janice Almeida Engler Pedro Augusto Braga Reis Maria Fátima Grossi-de-Sá Elizabeth Pacheco Batista Fontes Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses
description	Abstract NACs are plant-specific transcription factors involved in controlling plant development, stress responses, and senescence. As senescence-associated genes (SAGs), NACs integrate age- and stress-dependent pathways that converge to programmed cell death (PCD). In Arabidopsis, NAC-SAGs belong to well-characterized regulatory networks, poorly understood in soybean. Here, we interrogated the soybean genome and provided a comprehensive analysis of senescence-associated Glycine max (Gm) NACs. To functionally examine GmNAC-SAGs, we selected GmNAC065, a putative ortholog of Arabidopsis ANAC083/VNI2 SAG, and the cell death-promoting GmNAC085, an ANAC072 SAG putative ortholog, for analyses. Expression analysis of GmNAC065 and GmNAC085 in soybean demonstrated (i) these cell death-promoting GmNACs display contrasting expression changes during age- and stress-induced senescence; (ii) they are co-expressed with functionally different gene sets involved in stress and PCD, and (iii) are differentially induced by PCD inducers. Furthermore, we demonstrated GmNAC065 expression delays senescence in Arabidopsis, a phenotype associated with enhanced oxidative performance under multiple stresses, higher chlorophyll, carotenoid and sugar contents, and lower stress-induced PCD compared to wild-type. In contrast, GmNAC085 accelerated stress-induced senescence, causing enhanced chlorophyll loss, ROS accumulation and cell death, decreased antioxidative system expression and activity. Accordingly, GmNAC065 and GmNAC085 targeted functionally contrasting sets of downstream AtSAGs, further indicating that GmNAC85 and GmNAC065 regulators function inversely in developmental and environmental PCD.
format	article
author	Bruno Paes Melo Isabela Tristan Lourenço-Tessutti Otto Teixeira Fraga Luanna Bezerra Pinheiro Camila Barrozo de Jesus Lins Carolina Vianna Morgante Janice Almeida Engler Pedro Augusto Braga Reis Maria Fátima Grossi-de-Sá Elizabeth Pacheco Batista Fontes
author_facet	Bruno Paes Melo Isabela Tristan Lourenço-Tessutti Otto Teixeira Fraga Luanna Bezerra Pinheiro Camila Barrozo de Jesus Lins Carolina Vianna Morgante Janice Almeida Engler Pedro Augusto Braga Reis Maria Fátima Grossi-de-Sá Elizabeth Pacheco Batista Fontes
author_sort	Bruno Paes Melo
title	Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses
title_short	Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses
title_full	Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses
title_fullStr	Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses
title_full_unstemmed	Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses
title_sort	contrasting roles of gmnac065 and gmnac085 in natural senescence, plant development, multiple stresses and cell death responses
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eeaf472185ca4f26aca654521a107d93
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Contrasting roles of GmNAC065 and GmNAC085 in natural senescence, plant development, multiple stresses and cell death responses

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