Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.

The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, the mosquito microbial flora is diverse, composed...

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Autores principales:	Majoline T Tchioffo, Anne Boissière, Thomas S Churcher, Luc Abate, Geoffrey Gimonneau, Sandrine E Nsango, Parfait H Awono-Ambéné, Richard Christen, Antoine Berry, Isabelle Morlais
Formato:	article
Lenguaje:	EN
Publicado:	Public Library of Science (PLoS) 2013
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eeb264c55dde468b9178ffdd59e96ecc
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spelling	oai:doaj.org-article:eeb264c55dde468b9178ffdd59e96ecc2021-11-18T08:43:08ZModulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.1932-620310.1371/journal.pone.0081663https://doaj.org/article/eeb264c55dde468b9178ffdd59e96ecc2013-01-01T00:00:00Zhttps://www.ncbi.nlm.nih.gov/pmc/articles/pmid/24324714/?tool=EBIhttps://doaj.org/toc/1932-6203The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, the mosquito microbial flora is diverse, composed of commensal and symbiotic bacteria. We report here the isolation of culturable midgut bacteria from mosquitoes collected in the field in Cameroon and their identification based on the 16S rRNA gene sequencing. We next measured the effect of selected natural bacterial isolates on Plasmodium falciparum infection prevalence and intensity over multiple infectious feedings and found that the bacteria significantly reduced the prevalence and intensity of infection. These results contrast with our previous study where the abundance of Enterobacteriaceae positively correlated with P. falciparum infection (Boissière et al. 2012). The oral infection of bacteria probably led to the disruption of the gut homeostasis and activated immune responses, and this pinpoints the importance of studying microbe-parasite interactions in natural conditions. Our results indicate that the effect of bacterial exposure on P. falciparum infection varies with factors from the parasite and the human host and calls for deeper dissection of these parameters for accurate interpretation of bacterial exposure results in laboratory settings.Majoline T TchioffoAnne BoissièreThomas S ChurcherLuc AbateGeoffrey GimonneauSandrine E NsangoParfait H Awono-AmbénéRichard ChristenAntoine BerryIsabelle MorlaisPublic Library of Science (PLoS)articleMedicineRScienceQENPLoS ONE, Vol 8, Iss 12, p e81663 (2013)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Majoline T Tchioffo Anne Boissière Thomas S Churcher Luc Abate Geoffrey Gimonneau Sandrine E Nsango Parfait H Awono-Ambéné Richard Christen Antoine Berry Isabelle Morlais Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.
description	The development of Plasmodium falciparum within the Anopheles gambiae mosquito relies on complex vector-parasite interactions, however the resident midgut microbiota also plays an important role in mediating parasite infection. In natural conditions, the mosquito microbial flora is diverse, composed of commensal and symbiotic bacteria. We report here the isolation of culturable midgut bacteria from mosquitoes collected in the field in Cameroon and their identification based on the 16S rRNA gene sequencing. We next measured the effect of selected natural bacterial isolates on Plasmodium falciparum infection prevalence and intensity over multiple infectious feedings and found that the bacteria significantly reduced the prevalence and intensity of infection. These results contrast with our previous study where the abundance of Enterobacteriaceae positively correlated with P. falciparum infection (Boissière et al. 2012). The oral infection of bacteria probably led to the disruption of the gut homeostasis and activated immune responses, and this pinpoints the importance of studying microbe-parasite interactions in natural conditions. Our results indicate that the effect of bacterial exposure on P. falciparum infection varies with factors from the parasite and the human host and calls for deeper dissection of these parameters for accurate interpretation of bacterial exposure results in laboratory settings.
format	article
author	Majoline T Tchioffo Anne Boissière Thomas S Churcher Luc Abate Geoffrey Gimonneau Sandrine E Nsango Parfait H Awono-Ambéné Richard Christen Antoine Berry Isabelle Morlais
author_facet	Majoline T Tchioffo Anne Boissière Thomas S Churcher Luc Abate Geoffrey Gimonneau Sandrine E Nsango Parfait H Awono-Ambéné Richard Christen Antoine Berry Isabelle Morlais
author_sort	Majoline T Tchioffo
title	Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.
title_short	Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.
title_full	Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.
title_fullStr	Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.
title_full_unstemmed	Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.
title_sort	modulation of malaria infection in anopheles gambiae mosquitoes exposed to natural midgut bacteria.
publisher	Public Library of Science (PLoS)
publishDate	2013
url	https://doaj.org/article/eeb264c55dde468b9178ffdd59e96ecc
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Modulation of malaria infection in Anopheles gambiae mosquitoes exposed to natural midgut bacteria.

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