Genomes of Abundant and Widespread Viruses from the Deep Ocean

ABSTRACT The deep sea is a massive, largely oligotrophic ecosystem, stretched over nearly 65% of the planet’s surface. Deep-sea planktonic communities are almost completely dependent upon organic carbon sinking from the productive surface, forming a vital component of global biogeochemical cycles. H...

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Autores principales:	Carolina Megumi Mizuno, Rohit Ghai, Aurélien Saghaï, Purificación López-García, Francisco Rodriguez-Valera
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Lenguaje:	EN
Publicado:	American Society for Microbiology 2016
Materias:	Microbiology QR1-502
Acceso en línea:	https://doaj.org/article/eebc30d62ed24a36a6a068255ec8a6e4
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spelling	oai:doaj.org-article:eebc30d62ed24a36a6a068255ec8a6e42021-11-15T15:50:18ZGenomes of Abundant and Widespread Viruses from the Deep Ocean10.1128/mBio.00805-162150-7511https://doaj.org/article/eebc30d62ed24a36a6a068255ec8a6e42016-09-01T00:00:00Zhttps://journals.asm.org/doi/10.1128/mBio.00805-16https://doaj.org/toc/2150-7511ABSTRACT The deep sea is a massive, largely oligotrophic ecosystem, stretched over nearly 65% of the planet’s surface. Deep-sea planktonic communities are almost completely dependent upon organic carbon sinking from the productive surface, forming a vital component of global biogeochemical cycles. However, despite their importance, viruses from the deep ocean remain largely unknown. Here, we describe the first complete genomes of deep-sea viruses assembled from metagenomic fosmid libraries. “Candidatus Pelagibacter” (SAR11) phage HTVC010P and Puniceispirillum phage HMO-2011 are considered the most abundant cultured marine viruses known to date. Remarkably, some of the viruses described here recruited as many reads from deep waters as these viruses do in the photic zone, and, considering the gigantic scale of the bathypelagic habitat, these genomes provide information about what could be some of the most abundant viruses in the world at large. Their role in the viral shunt in the global ocean could be very significant. Despite the challenges encountered in inferring the identity of their hosts, we identified one virus predicted to infect members of the globally distributed SAR11 cluster. We also identified a number of putative proviruses from diverse taxa, including deltaproteobacteria, bacteroidetes, SAR11, and gammaproteobacteria. Moreover, our findings also indicate that lysogeny is the preferred mode of existence for deep-sea viruses inhabiting an energy-limited environment, in sharp contrast to the predominantly lytic lifestyle of their photic-zone counterparts. Some of the viruses show a widespread distribution, supporting the tenet “everything is everywhere” for the deep-ocean virome. IMPORTANCE The deep sea is among the largest known habitats and a critical cog in biogeochemical cycling but remains underexplored in its microbiology. Even more than is the case for its prokaryotic community, our knowledge of its viral component has remained limited by the paucity of information provided by studies dependent upon short sequence fragments. In this work, we attempt to fill this existing gap by using a combination of classical fosmid libraries with next-generation sequencing and assembly to recover long viral genomic fragments. We have sequenced ca. 6,000 fosmids from two metagenomics libraries made from prokaryotic biomass from the deep Mediterranean Sea and recovered twenty-eight complete viral genomes, all of them novel and quite distinct from all previously described viral genomes. They are preferentially found in deeper waters and are widely distributed all over the oceans. To our knowledge, this is the first report on complete and cosmopolitan viral genomes from the bathypelagic habitat.Carolina Megumi MizunoRohit GhaiAurélien SaghaïPurificación López-GarcíaFrancisco Rodriguez-ValeraAmerican Society for MicrobiologyarticleMicrobiologyQR1-502ENmBio, Vol 7, Iss 4 (2016)
institution	DOAJ
collection	DOAJ
language	EN
topic	Microbiology QR1-502
spellingShingle	Microbiology QR1-502 Carolina Megumi Mizuno Rohit Ghai Aurélien Saghaï Purificación López-García Francisco Rodriguez-Valera Genomes of Abundant and Widespread Viruses from the Deep Ocean
description	ABSTRACT The deep sea is a massive, largely oligotrophic ecosystem, stretched over nearly 65% of the planet’s surface. Deep-sea planktonic communities are almost completely dependent upon organic carbon sinking from the productive surface, forming a vital component of global biogeochemical cycles. However, despite their importance, viruses from the deep ocean remain largely unknown. Here, we describe the first complete genomes of deep-sea viruses assembled from metagenomic fosmid libraries. “Candidatus Pelagibacter” (SAR11) phage HTVC010P and Puniceispirillum phage HMO-2011 are considered the most abundant cultured marine viruses known to date. Remarkably, some of the viruses described here recruited as many reads from deep waters as these viruses do in the photic zone, and, considering the gigantic scale of the bathypelagic habitat, these genomes provide information about what could be some of the most abundant viruses in the world at large. Their role in the viral shunt in the global ocean could be very significant. Despite the challenges encountered in inferring the identity of their hosts, we identified one virus predicted to infect members of the globally distributed SAR11 cluster. We also identified a number of putative proviruses from diverse taxa, including deltaproteobacteria, bacteroidetes, SAR11, and gammaproteobacteria. Moreover, our findings also indicate that lysogeny is the preferred mode of existence for deep-sea viruses inhabiting an energy-limited environment, in sharp contrast to the predominantly lytic lifestyle of their photic-zone counterparts. Some of the viruses show a widespread distribution, supporting the tenet “everything is everywhere” for the deep-ocean virome. IMPORTANCE The deep sea is among the largest known habitats and a critical cog in biogeochemical cycling but remains underexplored in its microbiology. Even more than is the case for its prokaryotic community, our knowledge of its viral component has remained limited by the paucity of information provided by studies dependent upon short sequence fragments. In this work, we attempt to fill this existing gap by using a combination of classical fosmid libraries with next-generation sequencing and assembly to recover long viral genomic fragments. We have sequenced ca. 6,000 fosmids from two metagenomics libraries made from prokaryotic biomass from the deep Mediterranean Sea and recovered twenty-eight complete viral genomes, all of them novel and quite distinct from all previously described viral genomes. They are preferentially found in deeper waters and are widely distributed all over the oceans. To our knowledge, this is the first report on complete and cosmopolitan viral genomes from the bathypelagic habitat.
format	article
author	Carolina Megumi Mizuno Rohit Ghai Aurélien Saghaï Purificación López-García Francisco Rodriguez-Valera
author_facet	Carolina Megumi Mizuno Rohit Ghai Aurélien Saghaï Purificación López-García Francisco Rodriguez-Valera
author_sort	Carolina Megumi Mizuno
title	Genomes of Abundant and Widespread Viruses from the Deep Ocean
title_short	Genomes of Abundant and Widespread Viruses from the Deep Ocean
title_full	Genomes of Abundant and Widespread Viruses from the Deep Ocean
title_fullStr	Genomes of Abundant and Widespread Viruses from the Deep Ocean
title_full_unstemmed	Genomes of Abundant and Widespread Viruses from the Deep Ocean
title_sort	genomes of abundant and widespread viruses from the deep ocean
publisher	American Society for Microbiology
publishDate	2016
url	https://doaj.org/article/eebc30d62ed24a36a6a068255ec8a6e4
work_keys_str_mv	AT carolinamegumimizuno genomesofabundantandwidespreadvirusesfromthedeepocean AT rohitghai genomesofabundantandwidespreadvirusesfromthedeepocean AT aureliensaghai genomesofabundantandwidespreadvirusesfromthedeepocean AT purificacionlopezgarcia genomesofabundantandwidespreadvirusesfromthedeepocean AT franciscorodriguezvalera genomesofabundantandwidespreadvirusesfromthedeepocean
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Genomes of Abundant and Widespread Viruses from the Deep Ocean

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