Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter

Abstract Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant,...

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Autores principales:	Marina L. Mechler-Dreibi, Henrique M. S. Almeida, Karina Sonalio, Mariela A. C. Martines, Fernando A. M. Petri, Beatriz B. Zambotti, Marcela M. Ferreira, Gabriel Y. Storino, Tereza S. Martins, Hélio J. Montassier, Osvaldo A. Sant’Anna, Márcia C. A. Fantini, Luís Guilherme de Oliveira
Formato:	article
Lenguaje:	EN
Publicado:	Nature Portfolio 2021
Materias:	Medicine R Science Q
Acceso en línea:	https://doaj.org/article/eec01d86d9864139a3f60aa2d4f88c8f
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spelling	oai:doaj.org-article:eec01d86d9864139a3f60aa2d4f88c8f2021-11-21T12:20:25ZOral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter10.1038/s41598-021-01883-22045-2322https://doaj.org/article/eec01d86d9864139a3f60aa2d4f88c8f2021-11-01T00:00:00Zhttps://doi.org/10.1038/s41598-021-01883-2https://doaj.org/toc/2045-2322Abstract Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection.Marina L. Mechler-DreibiHenrique M. S. AlmeidaKarina SonalioMariela A. C. MartinesFernando A. M. PetriBeatriz B. ZambottiMarcela M. FerreiraGabriel Y. StorinoTereza S. MartinsHélio J. MontassierOsvaldo A. Sant’AnnaMárcia C. A. FantiniLuís Guilherme de OliveiraNature PortfolioarticleMedicineRScienceQENScientific Reports, Vol 11, Iss 1, Pp 1-15 (2021)
institution	DOAJ
collection	DOAJ
language	EN
topic	Medicine R Science Q
spellingShingle	Medicine R Science Q Marina L. Mechler-Dreibi Henrique M. S. Almeida Karina Sonalio Mariela A. C. Martines Fernando A. M. Petri Beatriz B. Zambotti Marcela M. Ferreira Gabriel Y. Storino Tereza S. Martins Hélio J. Montassier Osvaldo A. Sant’Anna Márcia C. A. Fantini Luís Guilherme de Oliveira Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
description	Abstract Mycoplasma (M.) hyopneumoniae is the main pathogen of porcine enzootic pneumonia (PEP). Its controlling is challenging, and requires alternative strategies. This study aimed to develop an oral vaccine against M. hyopneumoniae using a nanostructured mesoporous silica (SBA-15) as an adjuvant, and compare its effect with an intramuscular (IM) commercial vaccine (CV). Fifty 24 day-old M. hyopneumoniae-free piglets composed five equal groups for different immunization protocols, consisting of a CV and/or oral immunization (OI). Control piglets did not receive any form of immunization. All piglets were challenged with M. hyopneumoniae strain 232 on D49 by tracheal route. IgA antibody response in the respiratory tract, bacterial shedding and serum IgG were evaluated. The piglets were euthanized on 28 (D77) and 56 (D105) days post-infection. Lung lesions were macroscopically evaluated; lung fragments and bronchoalveolar fluid (BALF) were collected for estimation of bacterial loads by qPCR and/or histopathology examination. All immunization protocols induced reduction on Mycoplasma-like macroscopic lung lesions. IgA Ab responses anti-M. hyopneumoniae, the expression of IL-4 cytokine and a lower expression of IL-8 were induced by CV and OI vaccines, while IgG was induced only by CV. Oral immunization using silica as a carrier-adjuvant can be viable in controlling M. hyopneumoniae infection.
format	article
author	Marina L. Mechler-Dreibi Henrique M. S. Almeida Karina Sonalio Mariela A. C. Martines Fernando A. M. Petri Beatriz B. Zambotti Marcela M. Ferreira Gabriel Y. Storino Tereza S. Martins Hélio J. Montassier Osvaldo A. Sant’Anna Márcia C. A. Fantini Luís Guilherme de Oliveira
author_facet	Marina L. Mechler-Dreibi Henrique M. S. Almeida Karina Sonalio Mariela A. C. Martines Fernando A. M. Petri Beatriz B. Zambotti Marcela M. Ferreira Gabriel Y. Storino Tereza S. Martins Hélio J. Montassier Osvaldo A. Sant’Anna Márcia C. A. Fantini Luís Guilherme de Oliveira
author_sort	Marina L. Mechler-Dreibi
title	Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_short	Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_full	Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_fullStr	Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_full_unstemmed	Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
title_sort	oral vaccination of piglets against mycoplasma hyopneumoniae using silica sba-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter
publisher	Nature Portfolio
publishDate	2021
url	https://doaj.org/article/eec01d86d9864139a3f60aa2d4f88c8f
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Oral vaccination of piglets against Mycoplasma hyopneumoniae using silica SBA-15 as an adjuvant effectively reduced consolidation lung lesions at slaughter

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