Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia
Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence ana...
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oai:doaj.org-article:eed3680690874fbc8b024026e080e02c2021-11-17T21:27:54ZGenetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia1820-744810.2478/acve-2021-0003https://doaj.org/article/eed3680690874fbc8b024026e080e02c2021-03-01T00:00:00Zhttps://doi.org/10.2478/acve-2021-0003https://doaj.org/toc/1820-7448Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars.Jakov NišavićNenad MilićAndrea RadaljDejan KrnjaićDragan MilićevićAleksandra KneževićMarina RadojičićSonja ObrenovićMilivoje ĆosićBojana TešovićDamir BenkovićAleksandar ŽivuljSciendoarticleppv1ppv2ppv3wild boardna sequencingVeterinary medicineSF600-1100ENActa Veterinaria, Vol 71, Iss 1, Pp 32-46 (2021) |
| institution |
DOAJ |
| collection |
DOAJ |
| language |
EN |
| topic |
ppv1 ppv2 ppv3 wild boar dna sequencing Veterinary medicine SF600-1100 |
| spellingShingle |
ppv1 ppv2 ppv3 wild boar dna sequencing Veterinary medicine SF600-1100 Jakov Nišavić Nenad Milić Andrea Radalj Dejan Krnjaić Dragan Milićević Aleksandra Knežević Marina Radojičić Sonja Obrenović Milivoje Ćosić Bojana Tešović Damir Benković Aleksandar Živulj Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia |
| description |
Porcine parvoviruses (PPVs) are diverse and persistently evolving viruses found in domestic pigs and wild boars. Porcine parvovirus 1 (PPV1) causes reproductive problems in adult animals, although the veterinary relevance of PPV2, PPV3, and PPV4 has not been clarified. The detection and sequence analysis of PPVs circulating in wild boar populations in Serbia was performed to determine their phylogenetic relationships and prevalence in 122 organ samples collected during 2018. The DNA of PPV1, PPV2, and PPV3 was detected in 56.6% of the examined samples, whilst PPV4 was not identified. Overall, PPV3 was the most prevalent in 69.6% of the positive samples, followed by PPV1 in 63.8%, and PPV2 in 21.7% samples. Single infections were more common, although concurrent infections were confirmed in 34.8% samples for two, and 10.1% samples for three viruses. Sequence analysis of wild boar PPV1 showed no significant nucleotide differences from domestic pig PPV1 strains detected in Europe and the USA, however separate clustering from strains from China and the NADL-2 strain was demonstrated. Examination of the selected PPV2 sequences might suggest a certain geographical distribution of genetically diverse PPV2 strains considering high similarities to the strains from neighboring countries, and variability in comparison with other reported PPV2 sequences from different parts of the world. Wild boar PPV3 sequences clustered separately from most of the strains detected in wild boars, as well as the original porcine hokovirus strain. It is further noted that genetically different PPV3 strains circulate amongst Serbian domestic pigs and wild boars. |
| format |
article |
| author |
Jakov Nišavić Nenad Milić Andrea Radalj Dejan Krnjaić Dragan Milićević Aleksandra Knežević Marina Radojičić Sonja Obrenović Milivoje Ćosić Bojana Tešović Damir Benković Aleksandar Živulj |
| author_facet |
Jakov Nišavić Nenad Milić Andrea Radalj Dejan Krnjaić Dragan Milićević Aleksandra Knežević Marina Radojičić Sonja Obrenović Milivoje Ćosić Bojana Tešović Damir Benković Aleksandar Živulj |
| author_sort |
Jakov Nišavić |
| title |
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia |
| title_short |
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia |
| title_full |
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia |
| title_fullStr |
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia |
| title_full_unstemmed |
Genetic Analysis and Distribution of Porcine Parvoviruses Detected in the Organs of Wild Boars in Serbia |
| title_sort |
genetic analysis and distribution of porcine parvoviruses detected in the organs of wild boars in serbia |
| publisher |
Sciendo |
| publishDate |
2021 |
| url |
https://doaj.org/article/eed3680690874fbc8b024026e080e02c |
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