Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy
β-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM patients periodically receive leucodepleted packed red blood cells (P-RBCs) stored for maximum 14 days. The aim of this study...
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2021
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oai:doaj.org-article:eed66b231ec7441d8880c08434895b202021-11-25T16:53:13ZComparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy10.3390/biom111116382218-273Xhttps://doaj.org/article/eed66b231ec7441d8880c08434895b202021-11-01T00:00:00Zhttps://www.mdpi.com/2218-273X/11/11/1638https://doaj.org/toc/2218-273Xβ-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM patients periodically receive leucodepleted packed red blood cells (P-RBCs) stored for maximum 14 days. The aim of this study was to compare two alternative routine procedures to prepare the optimal P-RBCs product, in order to identify differences in their content that may somehow affect patients’ health and quality of life (QoL). In method 1, blood was leucodepleted and then separated to obtain P-RBCs, while in method 2 blood was separated and leucodepleted after removal of plasma and buffycoat. Forty blood donors were enrolled in two independent centers; couples of phenotypically matched whole blood units were pooled, divided in two identical bags and processed in parallel following the two methods. Biochemical properties, electrolytes and metabolic composition were tested after 2, 7 and 14 days of storage. Units prepared with both methods were confirmed to have all the requirements necessary for βTM transfusion therapy. Nevertheless, RBCs count and Hb content were found to be higher in method-1, while P-RBCs obtained with method 2 contained less K<sup>+</sup>, iron and storage lesions markers. Based on these results, both methods should be tested in a clinical perspective study to determine a possible reduction of transfusion-related complications, improving the QoL of βTM patients, which often need transfusions for the entire lifespan.Davide SchiroliLucia MerolleEleonora QuartieriRoberta ChicchiTommaso FasanoTiziana De LucaGiuseppe MolinariStefano PulciniThelma A. PertinhezErminia Di BartolomeoRino BiguzziRoberto BaricchiChiara MarracciniMDPI AGarticleβ-thalassemiapacked red blood cellsmetabolitesiron overloadblood transfusionMicrobiologyQR1-502ENBiomolecules, Vol 11, Iss 1638, p 1638 (2021) |
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β-thalassemia packed red blood cells metabolites iron overload blood transfusion Microbiology QR1-502 |
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β-thalassemia packed red blood cells metabolites iron overload blood transfusion Microbiology QR1-502 Davide Schiroli Lucia Merolle Eleonora Quartieri Roberta Chicchi Tommaso Fasano Tiziana De Luca Giuseppe Molinari Stefano Pulcini Thelma A. Pertinhez Erminia Di Bartolomeo Rino Biguzzi Roberto Baricchi Chiara Marraccini Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy |
| description |
β-thalassemia major (βTM) patients require frequent blood transfusions, with consequences that span from allogenic reactions to iron overload. To minimize these effects, βTM patients periodically receive leucodepleted packed red blood cells (P-RBCs) stored for maximum 14 days. The aim of this study was to compare two alternative routine procedures to prepare the optimal P-RBCs product, in order to identify differences in their content that may somehow affect patients’ health and quality of life (QoL). In method 1, blood was leucodepleted and then separated to obtain P-RBCs, while in method 2 blood was separated and leucodepleted after removal of plasma and buffycoat. Forty blood donors were enrolled in two independent centers; couples of phenotypically matched whole blood units were pooled, divided in two identical bags and processed in parallel following the two methods. Biochemical properties, electrolytes and metabolic composition were tested after 2, 7 and 14 days of storage. Units prepared with both methods were confirmed to have all the requirements necessary for βTM transfusion therapy. Nevertheless, RBCs count and Hb content were found to be higher in method-1, while P-RBCs obtained with method 2 contained less K<sup>+</sup>, iron and storage lesions markers. Based on these results, both methods should be tested in a clinical perspective study to determine a possible reduction of transfusion-related complications, improving the QoL of βTM patients, which often need transfusions for the entire lifespan. |
| format |
article |
| author |
Davide Schiroli Lucia Merolle Eleonora Quartieri Roberta Chicchi Tommaso Fasano Tiziana De Luca Giuseppe Molinari Stefano Pulcini Thelma A. Pertinhez Erminia Di Bartolomeo Rino Biguzzi Roberto Baricchi Chiara Marraccini |
| author_facet |
Davide Schiroli Lucia Merolle Eleonora Quartieri Roberta Chicchi Tommaso Fasano Tiziana De Luca Giuseppe Molinari Stefano Pulcini Thelma A. Pertinhez Erminia Di Bartolomeo Rino Biguzzi Roberto Baricchi Chiara Marraccini |
| author_sort |
Davide Schiroli |
| title |
Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy |
| title_short |
Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy |
| title_full |
Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy |
| title_fullStr |
Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy |
| title_full_unstemmed |
Comparison of Two Alternative Procedures to Obtain Packed Red Blood Cells for β-Thalassemia Major Transfusion Therapy |
| title_sort |
comparison of two alternative procedures to obtain packed red blood cells for β-thalassemia major transfusion therapy |
| publisher |
MDPI AG |
| publishDate |
2021 |
| url |
https://doaj.org/article/eed66b231ec7441d8880c08434895b20 |
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